LOCUS pSR1759_PGK_JNKKTR(EE_m 8825 bp ds-DNA circular 22-AUG-2017 DEFINITION . FEATURES Location/Qualifiers promoter 6754..6819 /label="EM7 promoter" misc_feature 4934..4946 /label="c-Jun(1-281)" misc_recomb 4756..4772 /label="AttL1" promoter 4179..4179 /label="human PGK promoter" misc_feature 4967..5650 /label="Clover" LTR 2448..2628 /label="HIV LTR 1" gene 6820..7419 /label="puro (variant)" rep_origin 8368..8823 /label="M13 origin" misc_feature 4808..4810 /label="mutation" rep_origin 1087..1769 /label="ColE1 origin" misc_feature 4950..4960 /label="c-Jun(1-281)" misc_feature 3290..3523 /label="RRE" misc_feature 4910..4921 /label="c-Jun(1-281)" LTR 7610..7790 /label="HIV LTR 2" misc_feature 4023..4038 /label="cPPT" misc_recomb complement(5687..5701) /label="AttR2" misc_binding 2109..2131 /label="LacO" primer_bind 2175..2194 /label="T3" 3'UTR 5734..6309 /label="WPRE" promoter 4180..4694 /label="human PGK promoter" rep_origin complement(8373..8813) /label="F1 ori" primer_bind complement(8207..8224) /label="M13-fwd" misc_recomb complement(5677..5686) /label="AttL2" CDS 330..989 /label="AmpR" primer_bind complement(7864..7881) /label="SG53" misc_feature 4778..4909 /label="REP" primer_bind 2137..2157 /label="M13-rev" misc_feature 7540..7555 /label="cPPT" misc_feature 4892..4894 /label="first site" misc_feature 4948..4948 /label="c-Jun(1-281)" misc_recomb 4748..4755 /label="AttR1" CDS 8295..8363 /label="LacZ alpha" promoter 6372..6714 /label="SV40 early promoter" misc_feature 4913..4915 /label="second" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcAgggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc CACcGgTTCG TCGACTAGTC CAGTGTGGTG GAATTCTGCA 4741 GATATCAACA AGTTTGTACA AAAAAGCAGG CTCCACCATG AGTAACCCTA AGATCCTAAA 4801 ACAGAGCATa ACCTTGAACC TGGCCGACCC GGTGGGCAGT CTGAAGCCGC ACCTCCGCGC 4861 CAAGCGCTCG GCCGGTCTCA ACGAAGACGA GgaGCCATCT AAGAAGCTGG CGgaGCCGGA 4921 GGTGTCGTCA AGGCTGGAGC GCCTGACCCT CCAGTCCAGC actagtatgg tgagcaaggg 4981 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 5041 ccacaagttc agcgtCCGCg gcgagggcga gggcgatgcc accAACggca agctgaccct 5101 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 5161 cggctacggc gtggcctgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 5221 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctctttca aggacgacgg 5281 TACCtacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 5341 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 5401 cttcaacagc cacaacgtct atatcacggc cgacaagcag aagaacggca tcaaggctaa 5461 cttcaagatc cgccacaacG TTgaggacgg cagcgtgcag ctcgccgacc actaccagca 5521 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcCATca 5581 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 5641 gaccgccgcc taaGCGGCCG CACTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG 5701 TTGATATCCA GCACAGTGGC GGCCGCTCGA caatcaacct ctggattaca aaatttgtga 5761 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 5821 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 5881 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 5941 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 6001 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 6061 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 6121 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 6181 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 6241 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 6301 cctttgggcc gcctccccgc ctggAATTCT GCAGATATCC GGTTAGTAAT GAGTTTGGAA 6361 TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG 6421 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG 6481 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 6541 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 6601 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT 6661 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC 6721 TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT 6781 ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA TGACCGAGTA CAAGCCCACG 6841 GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC 6901 GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC 6961 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG 7021 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG 7081 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA 7141 CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC 7201 GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA 7261 GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC 7321 CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA 7381 CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG 7441 CGCCCGACCG AAAGGAGCGC ACGACCCCAT GCATCTCGAG GGCCCGGTAC Ctttaagacc 7501 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 7561 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 7621 gttagaccag atctgagcct gggagctctc tggctgccta gggaacccac tgcttaagcc 7681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7801 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7861 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 7921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 7981 ttatcatgtc tggctctagc tATCCCGCCC CTAACTCCGC CCatcccgcc cctaactccg 8041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 8101 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 8161 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt 8221 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 8281 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 8341 tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg 8401 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 8461 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 8521 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 8581 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 8641 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 8701 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 8761 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 8821 tttcc //