LOCUS       pSR1759_PGK_JNKKTR(EE_m 8825 bp ds-DNA     circular     22-AUG-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        6754..6819
                     /label="EM7 promoter"
     misc_feature    4934..4946
                     /label="c-Jun(1-281)"
     misc_recomb     4756..4772
                     /label="AttL1"
     promoter        4179..4179
                     /label="human PGK promoter"
     misc_feature    4967..5650
                     /label="Clover"
     LTR             2448..2628
                     /label="HIV LTR 1"
     gene            6820..7419
                     /label="puro (variant)"
     rep_origin      8368..8823
                     /label="M13 origin"
     misc_feature    4808..4810
                     /label="mutation"
     rep_origin      1087..1769
                     /label="ColE1 origin"
     misc_feature    4950..4960
                     /label="c-Jun(1-281)"
     misc_feature    3290..3523
                     /label="RRE"
     misc_feature    4910..4921
                     /label="c-Jun(1-281)"
     LTR             7610..7790
                     /label="HIV LTR 2"
     misc_feature    4023..4038
                     /label="cPPT"
     misc_recomb     complement(5687..5701)
                     /label="AttR2"
     misc_binding    2109..2131
                     /label="LacO"
     primer_bind     2175..2194
                     /label="T3"
     3'UTR           5734..6309
                     /label="WPRE"
     promoter        4180..4694
                     /label="human PGK promoter"
     rep_origin      complement(8373..8813)
                     /label="F1 ori"
     primer_bind     complement(8207..8224)
                     /label="M13-fwd"
     misc_recomb     complement(5677..5686)
                     /label="AttL2"
     CDS             330..989
                     /label="AmpR"
     primer_bind     complement(7864..7881)
                     /label="SG53"
     misc_feature    4778..4909
                     /label="REP"
     primer_bind     2137..2157
                     /label="M13-rev"
     misc_feature    7540..7555
                     /label="cPPT"
     misc_feature    4892..4894
                     /label="first site"
     misc_feature    4948..4948
                     /label="c-Jun(1-281)"
     misc_recomb     4748..4755
                     /label="AttR1"
     CDS             8295..8363
                     /label="LacZ alpha"
     promoter        6372..6714
                     /label="SV40 early promoter"
     misc_feature    4913..4915
                     /label="second"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcAgggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc CACcGgTTCG TCGACTAGTC CAGTGTGGTG GAATTCTGCA
     4741 GATATCAACA AGTTTGTACA AAAAAGCAGG CTCCACCATG AGTAACCCTA AGATCCTAAA
     4801 ACAGAGCATa ACCTTGAACC TGGCCGACCC GGTGGGCAGT CTGAAGCCGC ACCTCCGCGC
     4861 CAAGCGCTCG GCCGGTCTCA ACGAAGACGA GgaGCCATCT AAGAAGCTGG CGgaGCCGGA
     4921 GGTGTCGTCA AGGCTGGAGC GCCTGACCCT CCAGTCCAGC actagtatgg tgagcaaggg
     4981 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5041 ccacaagttc agcgtCCGCg gcgagggcga gggcgatgcc accAACggca agctgaccct
     5101 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     5161 cggctacggc gtggcctgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     5221 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctctttca aggacgacgg
     5281 TACCtacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     5341 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     5401 cttcaacagc cacaacgtct atatcacggc cgacaagcag aagaacggca tcaaggctaa
     5461 cttcaagatc cgccacaacG TTgaggacgg cagcgtgcag ctcgccgacc actaccagca
     5521 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcCATca
     5581 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     5641 gaccgccgcc taaGCGGCCG CACTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG
     5701 TTGATATCCA GCACAGTGGC GGCCGCTCGA caatcaacct ctggattaca aaatttgtga
     5761 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5821 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5881 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5941 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6001 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6061 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6121 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6181 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6241 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6301 cctttgggcc gcctccccgc ctggAATTCT GCAGATATCC GGTTAGTAAT GAGTTTGGAA
     6361 TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG
     6421 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG
     6481 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC
     6541 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA
     6601 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT
     6661 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC
     6721 TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT
     6781 ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA TGACCGAGTA CAAGCCCACG
     6841 GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC
     6901 GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC
     6961 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG
     7021 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG
     7081 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA
     7141 CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC
     7201 GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA
     7261 GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC
     7321 CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA
     7381 CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG
     7441 CGCCCGACCG AAAGGAGCGC ACGACCCCAT GCATCTCGAG GGCCCGGTAC Ctttaagacc
     7501 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7561 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7621 gttagaccag atctgagcct gggagctctc tggctgccta gggaacccac tgcttaagcc
     7681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7801 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7861 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7981 ttatcatgtc tggctctagc tATCCCGCCC CTAACTCCGC CCatcccgcc cctaactccg
     8041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8101 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8161 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8221 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8281 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8341 tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg
     8401 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8461 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8521 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8581 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8641 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8701 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8761 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     8821 tttcc
//