LOCUS       MSt637_BB3_hyg_KanOri_pyrG5´_linkerAE      5351 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     misc_feature    complement(join(4916..5351,1..143))
                     /label="found on Seq#1FS2_FS3"
     primer          60..76
                     /label=Seq_BB1_BW
     misc_feature    complement(124..>147)
                     /feature_type=Misc.
                     /label=FS3_Kanori_fw
     primer          <144..171
                     /label=FS3_Tcbh2_rev
     FS              complement(144..147)
                     /label=FS3
     terminator      complement(149..738)
                     /label=Tcbh2_short
     CDS             complement(739..1764)
                     /label=hph
     promoter        complement(1765..2502)
                     /label=Ppki
     primer          1869..1890
                     /label=3_ppki_rev
     primer          complement(1869..1890)
                     /label=3_ppki_fw
     mutation        complement(1879)
                     /label=TamingA->T
     primer          complement(2244..2267)
                     /label=2_ppki_fw
     primer          2244..2267
                     /label=2_ppki_rev
     mutation        complement(2256)
                     /label=TamingA->T
     primer          complement(2483..>2506)
                     /label=FS2_ppki_fw
     Vector          2503..2580
                     /origin="BB2_L_12_syn_A_E_17 (3.147bp circular)Target
                     vector: from BpiI cut at 413^414 to BpiI cut at 491^492,
                     78bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
     FS              complement(2507..2510)
                     /label="FS E"
     FS              complement(2537..2540)
                     /label="FS A"
     terminator      complement(2541..2580)
                     /label=BBa_B1007
     primer          <2581..2604
                     /label=FS1_FlankpyrG_rev
     FS              complement(2581..2584)
                     /label=FS1
     misc_feature    complement(<2585..3430)
                     /feature_type="Putative promoter 1000bp"
                     /source=AspGD
                     /ID=56726-mRNA
     Region          complement(2585..3567)
                     /label=5_pyrG_1kb_56726
     mRNA            complement(join(2667..3104,3164..3299,3374..3415))
                     /source=AspGD
                     /ID=128428-mRNA-T
                     /Parent=128428-mRNA
                     /source=AspGD
                     /ID=128428-mRNA-T-E3
                     /ID=128428-mRNA-T-E2
                     /ID=128428-mRNA-T-E1
     gene            complement(2667..3415)
                     /source=AspGD
                     /ID=128428-mRNA
                     /label=128428
     misc_feature    complement(3416..>3567)
                     /feature_type="Putative promoter 1000bp"
                     /source=AspGD
                     /ID=128428-mRNA
     primer          complement(3548..>3595)
                     /label=FS4_FlankpyrG_Fw
     Target          complement(3569..3591)
                     /label=pyrG.1_CRISPR
     misc_feature    <3592..3618
                     /feature_type=Misc.
                     /label=FS4_Kanori_rev
     FS              complement(3592..3595)
                     /label=FS4
     misc_feature    3596..3727
                     /label="found on Seq#2FS2_FS3"
     primer          complement(3705..3722)
                     /label=Seq_BB1_FW
     misc_feature    3728..4365
                     /label="found on Seq#2FS2_FS3"
     oriT            complement(3749..4364)
                     /label="ColE1 origin, high copy number"
     CDS             complement(4410..5228)
                     /label=KanR
ORIGIN
        1 AGGCTTTTGA CTTTCTGCTA TGGAGGTCAG GTATGATTTA AATGGTCAGT ATTGAGCCTC
       61 AGGAAACAGC TATGACATCA AGCTGACTAG ATAATCTAGC TGATCGTGGA CCGATCATAC
      121 GTATAATGCC GTAAGATCAC GCgAAGCTAT TCTGCATTAA CGTCAGAGCA CAGGCTGGTT
      181 AACATGACCA GACTGATCGC CATAGGCTCA TGCTCCGCAG CTGTAGGCAA TTTGGGTATG
      241 GTTTCCACGT GCACTTACAT GTCATGGAGA AGCGCAAGAG GTGGAGTAAT TGGAATCTAC
      301 CAGCTTGATC TATAAACAGA AATTTTGGTT GCGATGGATC AGTACAGCCA TGTTGCACCC
      361 CGTGTATCCT CATTCAAGCA AGGTTGCGCA GTTGGCAAAG CCCTGCTCCT GTCACGACGT
      421 GCTTTCGCTG GTACTCGGAG TTGTTATACG CTACTCGACT GCCGAATCGA TCTTTAGATC
      481 ACAGACACAG TTGACTTTAT TCTCGGAGTA TGTCTAATAG ACACTCGACA TTGTGGATAG
      541 ATGTTCTCTC TCTCTCACTA GCTACTGATC GACAAAGACC ATCCATGTGA ATTATAGTAT
      601 CGCTGGCAAC AATCCATCAG TTGCTCATTT GCGGTCTACC GACCTGCGAC AGACAACCAA
      661 AGTAGACAAA GACTCCGCCA ACCGGGAACC ACACCATCGC ACATCATTGT TTGAAGCCCG
      721 GTCACGAAAG CCATGCATCT ATTCCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC
      781 ACTATCGGCG AGTACTTCTA CACAGCCATC GGTCCAGACG GCCGCGCTTC TGCGGGCGAT
      841 TTGTGTACGC CCGACAGTCC CGGCTCCGGA TCGGACGATT GCGTCGCATC GACCCTGCGC
      901 CCAAGCTGCA TCATCGAAAT TGCCGTCAAC CAAGCTCTGA TAGAGTTGGT CAAGACCAAT
      961 GCGGAGCATA TACGCCCGGA GCCGCGGCGA TCCTGCAAGC TCCGGgTGCC TCCGCTCGAA
     1021 GTAGCGCGTC TGCTGCTCCA TACAAGCCAA CCACGGCCTC CAGAAGAAGA TGTTGGCGAC
     1081 CTCGTATTGG GAATCCCCGA ACATCGCCTC GCTCCAGTCA ATGACCGCTG TTATGCGGCC
     1141 ATTGTCCGTC AGGACATTGT TGGAGCCGAA ATCCGCGTGC ACGAGGTGCC GGACTTCGGG
     1201 GCAGTCCTCG GCCCAAAGCA TCAGCTCATC GAGAGCCTGC GCGACGGACG CACTGACGGT
     1261 GTCGTCCATC ACAGTTTGCC AGTGATACAC ATGGGGATCA GCAATCGCGC ATATGAAATC
     1321 ACGCCATGTA GTGTATTGAC CGATTCCTTG CGGTCCGAAT GGGCCGAACC CGCTCGTCTG
     1381 GCTgAGATCG GCCGCAGCGA TCGCATCCAT GGCCTCCGCG ACCGGCTGCA GAACAGCGGG
     1441 CAGTTCGGTT TCAGGCAGGT CTTGCAACGT GACACCCTGT GCACGGCGGG AGATGCAATA
     1501 GGTCAGGCTC TCGCTGAATT CCCCAATGTC AAGCACTTCC GGAATCGGGA GCGCGGCCGA
     1561 TGCAAAGTGC CGATAAACAT AACGATCTTT GTAGAAACCA TCGGCGCAGC TATTTACCCG
     1621 CAGGACATAT CCACGCCCTC CTACATCGAA GCTGAAAGCA CGAGATTCTT CGCCCTCCGA
     1681 GAGCTGCATC AGGTCGGAGA CGCTGTCGAA CTTTTCGATC AGgAACTTCT CGACAGACGT
     1741 CGCGGTGAGT TCAGGCTTTT TCATCTAGAG GTTAAGAGGT TCTTCCGGCT TCGCGATAAG
     1801 GTACTGGAGG AGAAACAAAA GTCAAGCCGG TCGGGAGGGG GGGAGAGAAA TCAGCAGGTG
     1861 AGGCTCACGA GTCAGGTCAT CGTCAAGCTC CCGTGAGTGA AAAAAGAGAC GACGATAAGG
     1921 CGATGCGTGC AAAAGAGATG CAGACAAAGG GGCAGATGGA CCAAAGGCAG ACAAGGATGG
     1981 AGAGGGAAAT AAATAAGGGA ACAAGGGCGG CTTCGACGAG GGAGAGAGAA AAAAAAAACA
     2041 ACCTGAGCTG CGGGAAGGCG AGCCAAGATT AGGTGATGCT GCGCGCTGAG TGTGCAGGTG
     2101 GTCTGGGGTG GAGGGAGGGG GGTGGCAGAG CCGGCAGCCG TCAGTGGTTG GATTTTTTTT
     2161 TTTTTTTTTC TCACTTCAAC CTCACCTTTT TTCCCCTCTC TCTCTCTCTC TCTCTCTCCT
     2221 CTCTCTATAC TGTACCTAGC AATAATAGCT CATGGACTCA CAGCAACGCT CTGTGAGCCA
     2281 ACCAAGGGCC CGGATTGGCC ACAGCAGCCA CGACAAAGCA CGGCATCCCT CCGTTGGTTC
     2341 GCTGCTCCCA TGGCGGCCGC TTTGCTCCAA CTCAGGCGAT GCTGCCCCTC CATCCTTGCC
     2401 CTCGCCTCGC CAGTGAGGGG CATTGAGTTG AGCGCTAACT GAGGGGCAGG CCAACGTGGa
     2461 ACAGCTgGGA TAAGGGGACC GGCCGCTAGT CTCACCGTTA TTCATGCTCC GGAGACCCTC
     2521 GAGGGATCCG GTCTCCGATC gcgaaaaaac cccgccctgt caggggcggg gttttttgcg
     2581 CTCCAGTTTC CTTGCCATTG ACTGTGCCAC TCCCGAGGTC CATTGGAGCT CGTATTCCCT
     2641 GCTGCACATG GAGAAATAGA CCACGTCAGT CAGATTGCCA TTGTCATTGC CGACCCTAGC
     2701 AGCACGCATA GTAAAATGCG TTGAATAGTA GTATACAGTA GAGATTATTA CATATAGCTG
     2761 GTATAGCCAA ACATCGCCAA TCACCTCAAT CACACCACGG ATTCAGTCCA AACTTAGAAG
     2821 TAGGAAATCA ACTCCCTCGC AACTCCAAAA GACGTCAAGC CCCAGAGAGC TCCCTGTGCA
     2881 AGCAAGTAGA AGCTGCCGTA CAAACGACCG CGACCCCGCG GCTTGCCGGA GTACACATAT
     2941 CCGTAAAGGA ACAGTGAGCG ACCGAGGACC CAGATGCTTC CGAGGCCAGT CGCCAACTGG
     3001 GGGTACTTCA GTCCAGCTAC CAGGAGGAAG AGCATAGTTT GGCTGGAGTT CTCAAGGAAG
     3061 TTGGCATGAG CGCGCTGAGC GCAGTTGAAC TGCTCGGCCT TGGGCTGCAC GGTTCGGAAT
     3121 GTATGTTAGC TCGAGGAATC CTGTTTGTGT GAGGTTGGCT TACGTTGGTC TTGCACTGCT
     3181 CTACGGTCGC ATAGCAGTGA GGGTAGGGGC AATCAGCTTC CTTGCGGAGA CGAGACACGA
     3241 CGGCGCCATG GACGAAGCTC AGGACGGGGA TGGCACCCAG AGCGACGGCA ATGACAGAGC
     3301 TGCAGGATAG TTAGCATCAG ACTTCATGGG GAGAATGGCA TTGTCTGCAA GGAATTGTAG
     3361 AATTGGCACA TACCCGTAGT TTTCAGGGAC GGTCAGGGTA AGCATGATGG GTGATATTTA
     3421 CTTGTGGTGT TCTCAGCTTG AATCTGCTAT GACTAGGCGG TATTAGGAAG TCTAGAAGAT
     3481 GCCGAAAGTA GTTCGATTTA TAGTCGGGCG ATTGATGGGG CGCAAGAGCG GCCGGATGCG
     3541 GATCCAGAGC ATCCTTGGCG GATCCCTtCC TCCAAGTCGC AATTGACCTA CAGCGgGCCG
     3601 CATATCATTC AGGACGAGCC TCAGACTCCA GCGTAACTGG ACTGCAATCA ACTCACTGGC
     3661 TCACCTTCCG GTCCACGATC AGCTAGAATC AAGCTGACTA GATAAACTGG CCGTCGTTTT
     3721 ACACGGGTGG GCCTTTCTTC GGTAGAAAAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
     3781 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
     3841 GGATCAAGAG CTACCAACTC TTTTTCCGAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
     3901 AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
     3961 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
     4021 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
     4081 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
     4141 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
     4201 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
     4261 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCATCG ATTTTTGTGA
     4321 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCAGAAAG GCCCACCCGA
     4381 AGGTGAGCCA GGTGATTACA TTTAGGTCCT CATTAGAAAA ACTCATCGAG CATCAAGTGA
     4441 AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT TTTGAAAAAG CCGTTTCTGT
     4501 AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG CAAGATCCTG GTATCGGTCT
     4561 GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT TCCCCTCGTC AAAAATAAGG
     4621 TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG GTGAGAATGG CAAGAGTTTA
     4681 TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC GCTCGTCATC AAAATCACTC
     4741 GCACCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG CGAGACGAAA TACGCGATCG
     4801 CCGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC GGCGCAGGAA CACTGCCAGC
     4861 GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA ATACCTGGAA TGCTGTTTTC
     4921 CCTGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG TACGGATAAA ATGCTTGATG
     4981 GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGCCTGA CCATCTCATC TGTAACATCA
     5041 TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG GCGCATCGGG CTTCCCATAC
     5101 AATCGATAGA TTGTCGCACC TGATTGCCCG ACATTATCGC GAGCCCATTT ATACCCATAT
     5161 AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCTTCAAGC AAGACGTTTC CCGTTGAATA
     5221 TGGCTCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT
     5281 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AAGTCAAAAG
     5341 CCTCCGGTCG G
//
