LOCUS       MSt638_BB3_hyg_KanOri_pyrG5´_linkerAF      5351 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     misc_feature    complement(join(4973..5351,1..259))
                     /label="found on Seq#2FS2_FS3"
     oriT            join(4974..5351,1..238)
                     /label="ColE1 origin, high copy number"
     Vector          join(3840..5351,1..391)
                     /origin="MST047_FS34_Kan_ori (1.923bp circular)Target
                     vector: from BpiI cut at 411^412 to BpiI cut at 391^392,
                     1.903bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     misc_feature    complement(260..391)
                     /label="found on Seq#2FS2_FS3"
     primer          265..282
                     /label=Seq_BB1_FW
     misc_feature    complement(369..>395)
                     /feature_type=Misc.
                     /label=FS4_Kanori_rev
     primer          <392..439
                     /label=FS4_FlankpyrG_Fw
     FS              392..395
                     /label=FS4
     Target          396..418
                     /label=pyrG.1_CRISPR
     misc_feature    <420..571
                     /feature_type="Putative promoter 1000bp"
                     /source=AspGD
                     /ID=128428-mRNA
     Region          420..1402
                     /label=5_pyrG_1kb_56726
     misc_feature    557..>1402
                     /feature_type="Putative promoter 1000bp"
                     /source=AspGD
                     /ID=56726-mRNA
     mRNA            join(572..613,688..823,883..1320)
                     /source=AspGD
                     /ID=128428-mRNA-T
                     /Parent=128428-mRNA
                     /source=AspGD
                     /ID=128428-mRNA-T-E3
                     /ID=128428-mRNA-T-E2
                     /ID=128428-mRNA-T-E1
     gene            572..1320
                     /source=AspGD
                     /ID=128428-mRNA
                     /label=128428
     primer          complement(1383..>1406)
                     /label=FS1_FlankpyrG_rev
     terminator      <1403..1439
                     /label=BBa_B1007
     FS              1440..1443
                     /label="FS A"
     FS              1470..1473
                     /label="FS F"
     FS              1474..1477
                     /label="FS 2"
     primer          <1481..1504
                     /label=FS2_ppki_fw
     promoter        1485..2222
                     /label=Ppki
     primer          1720..1743
                     /label=2_ppki_fw
     primer          complement(1720..1743)
                     /label=2_ppki_rev
     mutation        1731
                     /label=TamingA->T
     primer          complement(2097..2118)
                     /label=3_ppki_rev
     primer          2097..2118
                     /label=3_ppki_fw
     mutation        2108
                     /label=TamingA->T
     CDS             2223..3248
                     /label=hph
     terminator      3249..3838
                     /label=Tcbh2_short
     primer          complement(3816..>3843)
                     /label=FS3_Tcbh2_rev
     misc_feature    <3840..3863
                     /feature_type=Misc.
                     /label=FS3_Kanori_fw
     FS              3840..3843
                     /label=FS3
     misc_feature    3844..4422
                     /label="found on Seq#1FS2_FS3"
     primer          complement(3911..3927)
                     /label=Seq_BB1_BW
     CDS             4110..4928
                     /label=KanR
ORIGIN
        1 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
       61 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      121 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      181 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      241 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      301 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      361 GTCTGAGGCT CGTCCTGAAT GATATGCGGC cCGCTGTAGG TCAATTGCGA CTTGGAGGaA
      421 GGGATCCGCC AAGGATGCTC TGGATCCGCA TCCGGCCGCT CTTGCGCCCC ATCAATCGCC
      481 CGACTATAAA TCGAACTACT TTCGGCATCT TCTAGACTTC CTAATACCGC CTAGTCATAG
      541 CAGATTCAAG CTGAGAACAC CACAAGTAAA TATCACCCAT CATGCTTACC CTGACCGTCC
      601 CTGAAAACTA CGGGTATGTG CCAATTCTAC AATTCCTTGC AGACAATGCC ATTCTCCCCA
      661 TGAAGTCTGA TGCTAACTAT CCTGCAGCTC TGTCATTGCC GTCGCTCTGG GTGCCATCCC
      721 CGTCCTGAGC TTCGTCCATG GCGCCGTCGT GTCTCGTCTC CGCAAGGAAG CTGATTGCCC
      781 CTACCCTCAC TGCTATGCGA CCGTAGAGCA GTGCAAGACC AACGTAAGCC AACCTCACAC
      841 AAACAGGATT CCTCGAGCTA ACATACATTC CGAACCGTGC AGCCCAAGGC CGAGCAGTTC
      901 AACTGCGCTC AGCGCGCTCA TGCCAACTTC CTTGAGAACT CCAGCCAAAC TATGCTCTTC
      961 CTCCTGGTAG CTGGACTGAA GTACCCCCAG TTGGCGACTG GCCTCGGAAG CATCTGGGTC
     1021 CTCGGTCGCT CACTGTTCCT TTACGGATAT GTGTACTCCG GCAAGCCGCG GGGTCGCGGT
     1081 CGTTTGTACG GCAGCTTCTA CTTGCTTGCA CAGGGAGCTC TCTGGGGCTT GACGTCTTTT
     1141 GGAGTTGCGA GGGAGTTGAT TTCCTACTTC TAAGTTTGGA CTGAATCCGT GGTGTGATTG
     1201 AGGTGATTGG CGATGTTTGG CTATACCAGC TATATGTAAT AATCTCTACT GTATACTACT
     1261 ATTCAACGCA TTTTACTATG CGTGCTGCTA GGGTCGGCAA TGACAATGGC AATCTGACTG
     1321 ACGTGGTCTA TTTCTCCATG TGCAGCAGGG AATACGAGCT CCAATGGACC TCGGGAGTGG
     1381 CACAGTCAAT GGCAAGGAAA CTGGAGCGCA AAAAACCCCG CCCCTGACAG GGCGGGGTTT
     1441 TTTCGCGATC GGAGACCGGA TCCCTCGAGG GTCTCCGCTT CATGAATAAC GGTGAGACTA
     1501 GCGGCCGGTC CCCTTATCCc AGCTGTtCCA CGTTGGCCTG CCCCTCAGTT AGCGCTCAAC
     1561 TCAATGCCCC TCACTGGCGA GGCGAGGGCA AGGATGGAGG GGCAGCATCG CCTGAGTTGG
     1621 AGCAAAGCGG CCGCCATGGG AGCAGCGAAC CAACGGAGGG ATGCCGTGCT TTGTCGTGGC
     1681 TGCTGTGGCC AATCCGGGCC CTTGGTTGGC TCACAGAGCG TTGCTGTGAG TCCATGAGCT
     1741 ATTATTGCTA GGTACAGTAT AGAGAGAGGA GAGAGAGAGA GAGAGAGAGA GGGGAAAAAA
     1801 GGTGAGGTTG AAGTGAGAAA AAAAAAAAAA AAAATCCAAC CACTGACGGC TGCCGGCTCT
     1861 GCCACCCCCC TCCCTCCACC CCAGACCACC TGCACACTCA GCGCGCAGCA TCACCTAATC
     1921 TTGGCTCGCC TTCCCGCAGC TCAGGTTGTT TTTTTTTTCT CTCTCCCTCG TCGAAGCCGC
     1981 CCTTGTTCCC TTATTTATTT CCCTCTCCAT CCTTGTCTGC CTTTGGTCCA TCTGCCCCTT
     2041 TGTCTGCATC TCTTTTGCAC GCATCGCCTT ATCGTCGTCT CTTTTTTCAC TCACGGGAGC
     2101 TTGACGATGA CCTGACTCGT GAGCCTCACC TGCTGATTTC TCTCCCCCCC TCCCGACCGG
     2161 CTTGACTTTT GTTTCTCCTC CAGTACCTTA TCGCGAAGCC GGAAGAACCT CTTAACCTCT
     2221 AGATGAAAAA GCCTGAACTC ACCGCGACGT CTGTCGAGAA GTTcCTGATC GAAAAGTTCG
     2281 ACAGCGTCTC CGACCTGATG CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG
     2341 ATGTAGGAGG GCGTGGATAT GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG
     2401 ATCGTTATGT TTATCGGCAC TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA
     2461 TTGGGGAATT CAGCGAGAGC CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT
     2521 TGCAAGACCT GCCTGAAACC GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG
     2581 ATGCGATCGC TGCGGCCGAT CTcAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG
     2641 GAATCGGTCA ATACACTACA TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT
     2701 ATCACTGGCA AACTGTGATG GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG
     2761 AGCTGATGCT TTGGGCCGAG GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG
     2821 GCTCCAACAA TGTCCTGACG GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG
     2881 CGATGTTCGG GGATTCCCAA TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG
     2941 CTTGTATGGA GCAGCAGACG CGCTACTTCG AGCGGAGGCA cCCGGAGCTT GCAGGATCGC
     3001 CGCGGCTCCG GGCGTATATG CTCCGCATTG GTCTTGACCA ACTCTATCAG AGCTTGGTTG
     3061 ACGGCAATTT CGATGATGCA GCTTGGGCGC AGGGTCGATG CGACGCAATC GTCCGATCCG
     3121 GAGCCGGGAC TGTCGGGCGT ACACAAATCG CCCGCAGAAG CGCGGCCGTC TGGACCGATG
     3181 GCTGTGTAGA AGTACTCGCC GATAGTGGAA ACCGACGCCC CAGCACTCGT CCGAGGGCAA
     3241 AGGAATAGAT GCATGGCTTT CGTGACCGGG CTTCAAACAA TGATGTGCGA TGGTGTGGTT
     3301 CCCGGTTGGC GGAGTCTTTG TCTACTTTGG TTGTCTGTCG CAGGTCGGTA GACCGCAAAT
     3361 GAGCAACTGA TGGATTGTTG CCAGCGATAC TATAATTCAC ATGGATGGTC TTTGTCGATC
     3421 AGTAGCTAGT GAGAGAGAGA GAACATCTAT CCACAATGTC GAGTGTCTAT TAGACATACT
     3481 CCGAGAATAA AGTCAACTGT GTCTGTGATC TAAAGATCGA TTCGGCAGTC GAGTAGCGTA
     3541 TAACAACTCC GAGTACCAGC GAAAGCACGT CGTGACAGGA GCAGGGCTTT GCCAACTGCG
     3601 CAACCTTGCT TGAATGAGGA TACACGGGGT GCAACATGGC TGTACTGATC CATCGCAACC
     3661 AAAATTTCTG TTTATAGATC AAGCTGGTAG ATTCCAATTA CTCCACCTCT TGCGCTTCTC
     3721 CATGACATGT AAGTGCACGT GGAAACCATA CCCAAATTGC CTACAGCTGC GGAGCATGAG
     3781 CCTATGGCGA TCAGTCTGGT CATGTTAACC AGCCTGTGCT CTGACGTTAA TGCAGAATAG
     3841 CTTcGCGTGA TCTTACGGCA TTATACGTAT GATCGGTCCA CGATCAGCTA GATTATCTAG
     3901 TCAGCTTGAT GTCATAGCTG TTTCCTGAGG CTCAATACTG ACCATTTAAA TCATACCTGA
     3961 CCTCCATAGC AGAAAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGAT CGGCACGTAA
     4021 GAGGTTCCAA CTTTCACCAT AATGAAATAA GATCACTACC GGGCGTATTT TTTGAGTTAT
     4081 CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGAGCCATAT TCAACGGGAA ACGTCTTGCT
     4141 TGAAGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG
     4201 ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGATTGTA TGGGAAGCCC GATGCGCCAG
     4261 AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA
     4321 GGCTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC
     4381 CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCAGGGAA AACAGCATTC CAGGTATTAG
     4441 AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT
     4501 TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACGGCGA TCGCGTATTT CGTCTCGCTC
     4561 AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGGTGCGAG TGATTTTGAT GACGAGCGTA
     4621 ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTCTTGCCA TTCTCACCGG
     4681 ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT
     4741 TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA
     4801 TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT
     4861 ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA CTTGATGCTC GATGAGTTTT
     4921 TCTAATGAGG ACCTAAATGT AATCACCTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTGC
     4981 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA TGCTCAAGTC
     5041 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     5101 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     5161 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     5221 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     5281 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     5341 CCACTGGTAA C
//
