LOCUS       BB1_T_34_ani_trpC_42      3708 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     Vector          join(2934..3708,1..2137)
                     /origin="pSTBLUE-1 wo_Amp_circular_BB1 (2.912bp
                     circular)Target vector: plasmid opened by EcoRV cut at
                     2137^2138Orientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     CDS             151..963
                     /label=KanamycinRes
     Region          1638..1695
                     /label="pUC origin"
     CDS             join(2037..2137,2934..>3004)
                     /label=lacZ
     Region          join(2087..2137,2934..>3004)
                     /label=mcs
     primer          2138..2166
                     /label=FS4_tTrpC_rev
     terminator      2138..2933
                     /origin="FS3_FS4_trpC_Anidulans_Seq (796bp
                     linear)Fragment: full sequenceOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label=trpC
     primer          complement(2555..2575)
                     /label=2_tTrpC_fw
     primer          2555..2575
                     /label=2_tTrpC_rev
     primer          complement(2897..2933)
                     /label=FS3_tTrpC_fw
     Region          <3005..3010
                     /label=mcs
     CDS             <3005..3210
                     /label=lacZ
     Region          complement(3205..3660)
                     /label="f1 origin"
ORIGIN
        1 GATCTCTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
       61 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGAACAAT AAAACTGTCT
      121 GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCTTGC
      181 TCTAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC
      241 GATAATGTCG GGCAATCAGG TGCGACAATC TATCGATTGT ATGGGAAGCC CGATGCGCCA
      301 GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC
      361 AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT
      421 CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGGA AAACAGCATT CCAGGTATTA
      481 GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG
      541 TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT
      601 CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT
      661 AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG
      721 GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA
      781 TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC
      841 ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA
      901 TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT
      961 TTCTAAGAAT TAATTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
     1021 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
     1081 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
     1141 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT
     1201 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
     1261 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
     1321 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
     1381 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
     1441 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
     1501 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
     1561 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
     1621 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
     1681 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT
     1741 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC
     1801 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC
     1861 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA
     1921 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
     1981 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA
     2041 CCATGATTAC GCCAAGCTCT AATACGACTC ACTATAGGGA AAGCTCGGTA CCACGCATGC
     2101 TGCAGACGCG TTACGTATCG GATCCAGAAT TCGTGATGAA GACGCAGCGA GTGGAGATGT
     2161 GGAGTGGGCG CTTACACAGT ACACGAGGAC TTCTAGAAAG AAGGATTACC TCTAAACAAG
     2221 TGTACCTGTG CATTCTGGGT AAACGACTCA TAGGAGAGTT GTAAAAAAGT TTCGGCCGGC
     2281 GTATTGGGTG TTACGGAGCA TTCACTAGGC AACCATGCAT GGTTACTATT GTATACCCAT
     2341 CTTAGTAGGA ATGATTTTCG AGGTTTATAC CTACGATGAA TGTGTGTCCT GTAGGCTTGA
     2401 GAGTTCAAGG AAGAAACAGT GCAATTATCT TTGCGAACCC AGGGGCTGGT GACGGAATTT
     2461 TCATAGTCAA GCTATCAGAG TAAAGAAGAG GAGCATGTCA AAGTACAATT AGAGACAAAT
     2521 ATATAGTCGC GTGGAGCCAA GAGCGGATTC CTCAGTCTCG TAGGACTCTT GACGACCGTT
     2581 GATCTGCTTG ATCTCGTCTC CCGAAAATGA AAATAGACTC TGCTAAGCTA TTCTTCTGCT
     2641 TCGCCGGAGC CTGAAGGGCG TACTAGGGTT GCGAGGTCCA ATGCATTAAT GCATTGCAGA
     2701 TGAGCTGTAT CTGGAAGAGG TAAACCCGAA ACGCGTTTTA TTCTTGTTGA CATGGAGCTA
     2761 TTAAATCACT AGAAGGCACT CTTTGCTGCT TGGACAAATG AACGTATCTT ATCGAGATCC
     2821 TGAACACCAT TTGTCTCAAC TCCGGAGCTG ACATCGACAC CAACGATCTT ATATCCAGAT
     2881 TCGTCAAGCT GTTTGATGAT TTCAGTAACG TTAAGTGGAT CAAGCGCGTC TTCATCTGAA
     2941 TTCGTCGACA AGCTTCTCGA GCCTAGGCTA GCTCTAGACC ACACGTGTGG GGGCCCGAGC
     3001 TCGCGGCCGC TGTATTCTAT AGTGTCACCT AAATGGCCGC ACAATTCACT GGCCGTCGTT
     3061 TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT
     3121 CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG
     3181 TTGCGCAGCC TGAATGGCGA ATGGAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT
     3241 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
     3301 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
     3361 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG
     3421 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC
     3481 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG
     3541 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
     3601 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
     3661 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTAA
//
