LOCUS       MSt045_BB1_FS34_AMA1_2      4816 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             join(4115..4816,1..117)
                     /label=KanR
     Vector          join(3830..4816,1..947)
                     /origin="BB1_L_34__BsaI_8 (1.966bp circular)Target
                     vector: from BsaI cut at 979^980 to BsaI cut at 947^948,
                     1.934bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     oriT            163..778
                     /label="ColE1 origin, high copy number"
     primer          805..822
                     /label=Seq_BB1_FW
     Site            join(<3830..3848,932..>947)
                     /site_type="cleavage site"
                     /label="Linker FS3 FS4"
     primer          <948..972
                     /label=FS3_AMA1/2_fw
     CDS             952..3829
                     /label=AMA1/2
     primer          1918..1941
                     /label=1_AMA1/2_fw
     primer          complement(1918..1941)
                     /label=1_AMA1/2_rev
     Taming          1931
                     /label=A>t
     primer          complement(3807..>3833)
                     /label=FS4_AMA1/2_rev
     FS              3830..3833
                     /label=FS4
     primer          complement(3916..3932)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGCT TATGCTGCAG
      961 ACGCGTTACG TATCGGATCC AGAATTCGTG ATATCTGAAT TCGTCGACAA GCTTATTTTT
     1021 TGTATACTGT TTTGTGATAG CACGAAGTTT TTCCACGGTA TCTTGTTAAA AATATATATT
     1081 TGTGGCGGGC TTACCTACAT CAAATTAATA AGAGACTAAT TATAAACTAA ACACACAAGC
     1141 AAGCTACTTT AGGGTAAAAG TTTATAAATG CTTTTGACGT ATAAACGTTG CTTGTATTTA
     1201 TTATTACAAT TAAAGGTGGA TAGAAAACCT AGAGACTAGT TAGAAACTAA TCTCAGGTTT
     1261 GCGTTAAACT AAATCAGAGC CCGAGAGGTT AACAGAACCT AGAAGGGGAC TAGATATCCG
     1321 GGTAGGGAAA CAAAAAAAAA AAACAAGACA GCCACATATT AGGGAGACTA GTTAGAAGCT
     1381 AGTTCCAGGA CTAGGAAAAT AAAAGACAAT GATACCACAG TCTAGTTGAC AACTAGATAG
     1441 ATTCTAGATT GAGGCCAAAG TCTCTGAGAT CCAGGTTAGT TGCAACTAAT ACTAGTTAGT
     1501 ATCTAGTCTC CTATAACTCT GAAGCTAGAA TAACTTACTA CTATTATCCT CACCACTGTT
     1561 CAGCTGCGCA AACGGAGTGA TTGCAAGGTG TTCAGAGACT AGTTATTGAC TAGTCAGTGA
     1621 CTAGCAATAA CTAACAAGGT ATTAACCTAC CATGTCTGCC ATCACCCTGC ACTTCCTCGG
     1681 GCTCAGCAGC CTTTTCCTCC TCATTTTCAT GCTCATTTTC CTTGTTTAAG ACTGTGACTA
     1741 GTCAAAGACT AGTCCAGAAC CACAAAGGAG AAATGTCTTA CCACTTTCTT CATTGCTTGT
     1801 CTCTTTTGCA TTATCCATGT CTGCAACTAG TTAGAGTCTA GTTAGTGACT AGTCCGACGA
     1861 GGACTTGCTT GTCTCCGGAT TGTTGGAGGA ACTCTCCAGG GCCTCAAGAT CCACAACAGA
     1921 GCCTTCTAGA tGACTGGTCA ATAACTAGTT GGTCTTTGTC TGAGTCTGAC TGACTTACGA
     1981 GGTTGCATAC TCGCTCCCTT TGCCTCGTCA ATCGATGAGA AAAAGCGCCA AAACTCGCAA
     2041 TATGGCTTTG AACCACACGG TGCTGAGACT AGTTAGAATC TAGTCCCAAA CTAGCTTGGA
     2101 TAGCTTACCT TTGCCCTTTG CGTTGCGACA GGTCTTGCAG GGTATGGTTC CTTTCTCACC
     2161 AGCTGATTTA GCTGCCTTGC TACCCTCACG GCGGATCTGC ATAAAGAGTG GCTAGAGGTT
     2221 ATAAATTAGC ACTGATCCTA GGTACGGGGC TGAATGTAAC TTGCCTTTCC TTTCTCATCG
     2281 CGCGGCAAGA CAGGCTTGCT CAAATTCCTA CCAGTCACAG GGGTATGCAC GGCGTACGGA
     2341 CCACTTGAAC TAGTCACAGA TTAGTTAGCA ACTAGTCTGC ATTGAATGGC TGTACTTACG
     2401 GGCCCTCGCC ATTGTCCTGA TCATTTCCAG CTTCACCCTC GTTGCTGCAA AGTAGTTAGT
     2461 GACTAGTCAA GGACTAGTTG AAATGGGAGA AGAAACTCAC GAATTCTCGA CACCCTTAGT
     2521 ATTGTGGTCC TTGGACTTGG TGCTGCTATA TATTAGCTAA TACACTAGTT AGACTCACAG
     2581 AAACTTACGC AGCTCGCTTG CGCTTCTTGG TAGGAGTCGG GGTTGGGAGA ACAGTGCCTT
     2641 CAAACAAGCC TTCATACCAT GCTACTTGAC TAGTCAGGGA CTAGTCACCA AGTAATCTAG
     2701 ATAGGACTTG CCTTTGGCCT CCATCAGTTC CTTCATAGTG GGAGGTCCAT TGTGCAATGT
     2761 AAACTCCATG CCGTGGGAGT TCTTGTCCTT CAAGTGCTTG ACCAATATGT TTCTGTTGGC
     2821 AGAGGGAACC TGTCAACTAG TTAATAACTA GTCAGAAACT AGTATAGCAG TAGACTCACT
     2881 GTACGCTTGA GGCATCCCTT CACTCGGCAG TAGACTTCAT ATGGATGGAT ATCAGGCACG
     2941 CCATTGTCGT CCTGTGGACT AGTCAGTAAC TAGGCTTAAA GCTAGTCGGG TCGGCTTACT
     3001 ATCTTGAAAT CCGGCAGCGT AAGCTCCCCG TCCTTAACTG CCTCGAGATA GTGACAGTAC
     3061 TCTGGGGACT TTCGGAGATC GTTATCGCGA ATGCTCGGCA TACTAATCGT TGACTAGTCT
     3121 TGGACTAGTC CCGAGCAAAA AGGATTGGAG GAGGAGGAGG AAGGTGAGAG TGAGACAAAG
     3181 AGCGAAATAA GAGCTTCAAA GGCTATCTCT AAGCAGTATG AAGGTTAAGT ATCTAGTTCT
     3241 TGACTAGATT TAAAAGAGAT TTCGACTAGT TATGTACCTG GAGTTTGGAT ATAGGAATGT
     3301 GTTGTGGTAA CGAAATGTAA GGGGGAGGAA AGAAAAAGTC GGTCAAGAGG TAACTCTAAG
     3361 TCGGCCATTC CTTTTTGGGA GGCGCTAACC ATAAACGGCA TGGTCGACTT AGAGTTAGCT
     3421 CAGGGAATTT AGGGAGTTAT CTGCGACCAC CGAGGAACGG CGGAATGCCA AAGAATCCCG
     3481 ATGGAGCTCT AGCTGGCGGT TGACAACCCC ACCTTTTGGC GTTTCTGCGG CGTTGCAGGC
     3541 GGGACTGGAT ACTTCGTAGA ACCAGAAAGG CAAGGCAGAA CGCGCTCAGC AAGAGTGTTG
     3601 GAAGTGATAG CATGATGTGC CTTGTTAACT AGGTCAAAAT CTGCAGTATG CTTGATGTTA
     3661 TCCAAAGTGT GAGAGAGGAA GGTCCAAACA TACACGATTG GGAGAGGGCC TAGGTATAAG
     3721 AGTTTTTGAG TAGAACGCAT GTGAGCCCAG CCATCTCGAG GAGATTAAAC ACGGGCCGGC
     3781 ATTTGATGGC TATGTTAGTA CCCCAATGGA AAGCCTGAGA GTCCAGTGGC GCTGCGTCTT
     3841 CGAATTCGCG CGTGATCTTA CGGCATTATA CGTATGATCG GTCCACGATC AGCTAGATTA
     3901 TCTAGTCAGC TTGATGTCAT AGCTGTTTCC TGAGGCTCAA TACTGACCAT TTAAATCATA
     3961 CCTGACCTCC ATAGCAGAAA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGATCGGCA
     4021 CGTAAGAGGT TCCAACTTTC ACCATAATGA AATAAGATCA CTACCGGGCG TATTTTTTGA
     4081 GTTATCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGAGC CATATTCAAC GGGAAACGTC
     4141 TTGCTTGAAG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC
     4201 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC
     4261 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT
     4321 GGTCAGGCTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG
     4381 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCA GGGAAAACAG CATTCCAGGT
     4441 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG
     4501 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC GGCGATCGCG TATTTCGTCT
     4561 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGGT GCGAGTGATT TTGATGACGA
     4621 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTCT TGCCATTCTC
     4681 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG
     4741 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT
     4801 TGCCATCCTA TGGAAC
//
