LOCUS pNBU2_erm_P1T_DP 5111 bp ds-DNA linear 04-MAY-2017 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 1280..2560 /label=NBU2 Integrase ORF /ApEinfo_fwdcolor="#50ff41" /ApEinfo_revcolor="#50ff41" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 967..1009 /label=rpiL* RBS site /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 910..913 /label=-33 binding site /ApEinfo_fwdcolor="#ff764f" /ApEinfo_revcolor="#ff764f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(886..904) /label=tetR binding site /ApEinfo_fwdcolor="#ffc061" /ApEinfo_revcolor="#ffc061" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2720..2732 /label=attN2 /ApEinfo_fwdcolor="#ff786c" /ApEinfo_revcolor="#ff786c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 935..942 /label=-7 binding site /ApEinfo_fwdcolor="#ff7e51" /ApEinfo_revcolor="#ff7e51" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 947..947 /label=+1 txn initiation site /ApEinfo_fwdcolor="#ffd544" /ApEinfo_revcolor="#ffd544" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4222..5083 /label=bla ORF /ApEinfo_fwdcolor="#6b92ff" /ApEinfo_revcolor="#6b92ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 948..966 /label=tetR binding site(1) /ApEinfo_label="tetR binding site" /ApEinfo_fwdcolor="#ffc061" /ApEinfo_revcolor="#ffc061" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1012..1014 /label=start codon /ApEinfo_fwdcolor="#b4ff9d" /ApEinfo_revcolor="#b4ff9d" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 10..795 /label=RP4 oriT/R6K oriV /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1196..1219 /label=3' sequencing primer /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#00ffff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3000..3734 /label=ermG ORF /ApEinfo_fwdcolor="#82ff8f" /ApEinfo_revcolor="#82ff8f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1062..1067 /label=SalI site /ApEinfo_fwdcolor="#543aff" /ApEinfo_revcolor="#543aff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 806..826 /label=5' sequencing primer /ApEinfo_fwdcolor="#f958ff" /ApEinfo_revcolor="#f958ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 CAATTCGAGG GGGATCAATT CCGTGATAGG TGGGCTGCCC TTCCTGGTTG GCTTGGTTTC 61 ATCAGCCATC CGCTTGCCCT CATCTGTTAC GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC 121 AGGATTCCCG TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT 181 CGCGGGTGGG CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA 241 GTCTACACGA ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA 301 AAAAATCGCT ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAACGGAA TTGATCCGGC 361 CACGATGCGT CCGGCGTAGA GGATCTGAAG ATCAGCAGTT CAACCTGTTG ATAGTACGTA 421 CTAAGCTCTC ATGTTTCACG TACTAAGCTC TCATGTTTAA CGTACTAAGC TCTCATGTTT 481 AACGAACTAA ACCCTCATGG CTAACGTACT AAGCTCTCAT GGCTAACGTA CTAAGCTCTC 541 ATGTTTCACG TACTAAGCTC TCATGTTTGA ACAATAAAAT TAATATAAAT CAGCAACTTA 601 AATAGCCTCT AAGGTTTTAA GTTTTATAAG AAAAAAAAGA ATATATAAGG CTTTTAAAGC 661 TTTTAAGGTT TAACGGTTGT GGACaacaag ccagggatgt aacgCACTGA GAAGCCCTTA 721 GAGCCTCTCA AAGCAATTTT GAGTGACACA GGAACACTTA ACGGCTGACA TGGGAATTCC 781 CCTCCACCGC GGTGGCTttt tgcacccgct ttccaagaga agaaagcctt gttaaattga 841 cttagtgtaa aagcgcagta ctgcttgacc ataagaacaa aaaaaTCTCT ATCACTGATA 901 GGGAtaaagT TTGgaagata aagctaaaag ttctTATCTT TGcagtCTCC CTATCAGTGA 961 TAGAGAcgca ttttaaaata aaataaatta tttatttaat taaacgaatc cATGGTGGAT 1021 CCCCCGGGCT GCAGGAATTC GATATCAAGC TTATCGATAC CGTCGACTAA TTGCCTATCT 1081 TCCAGTGATG GAACAGCATT TGTGCATTGG CTGCAACAAT CAGCCTTACT TGTGCCTGTT 1141 CTATTTCCGA ACCGACCGCT TGTATGAATC CATCAAAATT CGTTTTCTCT ATGTTGGATT 1201 CCTTGTTGCT CATATTGTGA TGATAATTTC TACAAATATA GTCATTGGTA ACTATCTATG 1261 AAACTGTTTG ATACTTTTAT AGTTGATTAA ACTTGTTCAT GGCATTTGCC TTAATATCAT 1321 CCGCTATGTC AATGTAGGGT TTCATAGCTT TGTAGTCGCT GTGTCCCGTC CATTTCATGA 1381 CCACCTGTGC CGGGATTCCG AGAGCCAGCG CATTGCAGAT GAATGTCCTT CTTCCTGCAT 1441 GGGTACTGAG CAAAGCGTAT TTGGGTGTGA CTTCATCAAT ACGTTCATTT CCCTTGTAGT 1501 AGGTTTCCCG TACAGGCTCG TTGATTTCTG CCAGTTCGCC CAGCTCTTTC AGGTAATCGT 1561 TCATCTTCTG GTTGCTGATG ACGGGCAGAG CCATGTAATT CTCGAAATGG ATGTCCTTGT 1621 ATTTGTCCAG TATGGCTTTG CTGTATTTGT TCAGTTCAAT CGTCAGGCTG TCGGCAGTCT 1681 TGACTGTGGT TATTTCGATG TGGTCGGACT TCACATCGCT TCTTTTCAGA TTGCGAACAT 1741 CCGAATACCG CAAACTCGTA AAGCAGCAGA ACAGGAAAAC ATCACGCACA CGTTCCAGGT 1801 ATTGCTTATC CTTGGGTATC TGGTAGTCTT TCAGCTTGTT CAGTTCATCC CAAGTCAGGA 1861 AGATTACTTT TTTCGAGGTG GTTTTCAGTT TCGGTTTGAA CGTATCGTAT GCAATGTTCT 1921 GATGATGTCC TTTCTTGAAG CTCCAGCGCA GGAACCATTT GAGGAATCCC ATTTGCTTGC 1981 CGATGGTGCT GTTTCTCATA TCCTTGGTGT CACGCAGGAA GTTGACGTAT TCGTTCAATC 2041 CAAACTCGTT GAAATAGTTG AACGTTGCAT CCTCCTTGAA CTCTTTGAGG TGGTTCCTCA 2101 CTGCTGCAAA TTTTTCATAG GTGGATGCCG TCCAGTTATT CTGGTTACCG CACTCTTTTA 2161 CAAACTCATC GAACACCTCC CAAAAGCTGA CAGGGGCTTC TTCCGGCTGT TCTTCGCTGG 2221 TGTCTTTCAT TCTCATGTTG AAAGCTTCCT TCAACTGTTG GGTCGTTGGC ATGACCTCCT 2281 GCACCTCAAA TTCCTTGAAA ATATTCTGGA TTTCGGCATA GTATTTCAGC AAGTCCGTAT 2341 TGATTTCGGC TGCACTTTGC TTTAGCTTGT TGGTACATCC GCTCTTTACC CGCTGCTTAT 2401 CTGCATCCCA TTTGGCTACG TCAATCCGGT AGCCCGTTGT AAACTCGATG CGTTGGCTGG 2461 CAAAGATGAC ACGCATACGG ATGGGTACGT TCTCTACGAT TGGCACACCG TTCTTTTTCC 2521 GGCTCTCCAA TGCAAAAATG ATGTTGCGCT TGATATTCAT AATTGGGTGC GTTTGAAATT 2581 CTACACCCAA ATATACACCC AATTATTGAG ATAGCAAAAG ACATTTAGAA ACATTTACTT 2641 TTACTCTATA TTGTAATTTA CACTTGATTA TCAGTCGTTT GCAGTCTTAT GATATTCTGT 2701 GAAAGTATAA GTTCGAGAGC CTGTCTCTCC GCAAAAAACG CTGAAAATCA GCAGATTGCA 2761 AAACAAACAC CCTGTTTTAC ACCCAAGAAT GTAAAGTCGG CTGTTTTTGT TTTATTTAAG 2821 ATAATACAAC CACTACATAA TAAAAGAGTA GCGATATTAA AAGAATCCGA TGAGAAAAGA 2881 CTAATATTTA TCTATCCATT CAGTTTGATT TTTCAGGACT TTACATCGTC CTGAAAGTAT 2941 TTGTTGGTAC CGGTACCGAG GACGCGtaaa catttacagt tgcatgtggc ctattgtttT 3001 TAGCCGTTAA ATATTTTATA ACTATTAAAT AGCGATACAA ATTGTTCGAA ACTAATATTG 3061 TTTATATCAT ATATTCTCGC ATGTTTTAAA GCTTTATTAA ATTGATTTTT TGTAAACAGT 3121 TTTTCGTACT CTTTGTTAAC CCATTTCATT ACAAAAGTTT CATATTTTTT TCTCTCTTTA 3181 AATGCCATTT TTGCTGGCTT TCTTTTTAAT ACAATTAATG TGCTATCCAC TTTAGGTTTT 3241 GGATGGAAAT AATACCTAGG AATTTTTGCT AATATAGAAA TATCTACCTC TGCCATTAAC 3301 AGCAATGCTA GTGATCTGTT TGTATCTAAT AACATTTTAG CAAAACCATA TTCCACTATT 3361 AAATAACTTA TTGTGGCTGA ACTTTCAAAA ACAATTTTTC GAATTATATT TGTGCTTATG 3421 TTGTAAGGTA TGCTGCCAAA TATTTTATAT GGATTGTGGC TAGGAAATGT AAATTTCAGT 3481 ATATCATCAT TTACTATTTG ATAGTTAGGA TAATTTAAGA GCTTATTACG AGTTACCTCA 3541 CATAATTTAG AATCAATTTC TATCGCCGTT ACAAAATTAC ATCTCTTTAC CAATCCAGCA 3601 GTAAAATGAC CTTTCCCTGC ACCTATTTCA AAGATGTTAT CTTTTTCATC TAAACTTATG 3661 CAATTCATTA TTTTTTCTAT GTGATATTTT GAAGTAATAA AATTTTGACT ATCTTTTATA 3721 TTTACTTTGT TCATTATAAC CTCTCCTTAA TTTATTGCAT CTCTTTTCGA ATATTTATGT 3781 TTTTTGAGAA AAGAACGTAC TCATGGTTCA TCCCGATATG CGTATCGGTC TGTATATCAG 3841 CAACTTTCTA TGTGTTTCAA CTACAATAGT CATCTATTCT CATCTTTCTG AGTCCACCCC 3901 CTGCAAAGCC CCTCtttacg acataaaaat tcggtCGGAA AAGGTATGCA AAAGATGTTT 3961 CTCTCTTTAA GAGAAACTCT TCGGGATGCA AAAATATGAA AATAACTCCA ATTCACCAAA 4021 TTATATAGCG ACTTTTTTAC AAAATGCTAA AATTTGTTGA TTTCCGTCAA GCAATTGTTG 4081 AGCAAAAATG TCTTTTACGA TAAAATGATA CCTCAATATC AACTGTTTAG CAAAACGATA 4141 TTTCTCTTAA AGAGAGAAAC ACCTTTTTGT TCACCAATCC CCGACTTTTA ATCCCGCGGC 4201 CATGATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 4261 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 4321 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 4381 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 4441 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 4501 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 4561 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 4621 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 4681 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 4741 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 4801 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 4861 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 4921 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 4981 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 5041 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 5101 TCATAACGCG T //