LOCUS       pNBU2_erm_P1T_DP        5111 bp ds-DNA     linear       04-MAY-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1280..2560
                     /label=NBU2 Integrase ORF
                     /ApEinfo_fwdcolor="#50ff41"
                     /ApEinfo_revcolor="#50ff41"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    967..1009
                     /label=B41 RBS site
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    910..913
                     /label=-33 binding site
                     /ApEinfo_fwdcolor="#ff764f"
                     /ApEinfo_revcolor="#ff764f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2720..2732
                     /label=attN2
                     /ApEinfo_fwdcolor="#ff786c"
                     /ApEinfo_revcolor="#ff786c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    935..942
                     /label=-7 binding site
                     /ApEinfo_fwdcolor="#ff7e51"
                     /ApEinfo_revcolor="#ff7e51"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    947..947
                     /label=+1 txn initiation site
                     /ApEinfo_fwdcolor="#ffd544"
                     /ApEinfo_revcolor="#ffd544"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4222..5083
                     /label=bla ORF
                     /ApEinfo_fwdcolor="#6b92ff"
                     /ApEinfo_revcolor="#6b92ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    948..966
                     /label=tetR binding site
                     /ApEinfo_fwdcolor="#ffc061"
                     /ApEinfo_revcolor="#ffc061"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1012..1014
                     /label=start codon
                     /ApEinfo_fwdcolor="#b4ff9d"
                     /ApEinfo_revcolor="#b4ff9d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10..795
                     /label=RP4 oriT/R6K oriV
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1196..1219
                     /label=3' sequencing primer
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3000..3734
                     /label=ermG ORF
                     /ApEinfo_fwdcolor="#82ff8f"
                     /ApEinfo_revcolor="#82ff8f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1062..1067
                     /label=SalI site
                     /ApEinfo_fwdcolor="#543aff"
                     /ApEinfo_revcolor="#543aff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    806..826
                     /label=5' sequencing primer
                     /ApEinfo_fwdcolor="#f958ff"
                     /ApEinfo_revcolor="#f958ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(886..904)
                     /label=tetR binding site(1)
                     /ApEinfo_label="tetR binding site"
                     /ApEinfo_fwdcolor="#ffc061"
                     /ApEinfo_revcolor="#ffc061"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CAATTCGAGG GGGATCAATT CCGTGATAGG TGGGCTGCCC TTCCTGGTTG GCTTGGTTTC
       61 ATCAGCCATC CGCTTGCCCT CATCTGTTAC GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC
      121 AGGATTCCCG TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT
      181 CGCGGGTGGG CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA
      241 GTCTACACGA ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA
      301 AAAAATCGCT ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAACGGAA TTGATCCGGC
      361 CACGATGCGT CCGGCGTAGA GGATCTGAAG ATCAGCAGTT CAACCTGTTG ATAGTACGTA
      421 CTAAGCTCTC ATGTTTCACG TACTAAGCTC TCATGTTTAA CGTACTAAGC TCTCATGTTT
      481 AACGAACTAA ACCCTCATGG CTAACGTACT AAGCTCTCAT GGCTAACGTA CTAAGCTCTC
      541 ATGTTTCACG TACTAAGCTC TCATGTTTGA ACAATAAAAT TAATATAAAT CAGCAACTTA
      601 AATAGCCTCT AAGGTTTTAA GTTTTATAAG AAAAAAAAGA ATATATAAGG CTTTTAAAGC
      661 TTTTAAGGTT TAACGGTTGT GGACaacaag ccagggatgt aacgCACTGA GAAGCCCTTA
      721 GAGCCTCTCA AAGCAATTTT GAGTGACACA GGAACACTTA ACGGCTGACA TGGGAATTCC
      781 CCTCCACCGC GGTGGCTttt tgcacccgct ttccaagaga agaaagcctt gttaaattga
      841 cttagtgtaa aagcgcagta ctgcttgacc ataagaacaa aaaaaTCTCT ATCACTGATA
      901 GGGAtaaagT TTGgaagata aagctaaaag ttctTATCTT TGcagtCTCC CTATCAGTGA
      961 TAGAGAcgca ttttaaaata aaataaatca tatagttaat taaacgaatc cATGGTGGAT
     1021 CCCCCGGGCT GCAGGAATTC GATATCAAGC TTATCGATAC CGTCGACTAA TTGCCTATCT
     1081 TCCAGTGATG GAACAGCATT TGTGCATTGG CTGCAACAAT CAGCCTTACT TGTGCCTGTT
     1141 CTATTTCCGA ACCGACCGCT TGTATGAATC CATCAAAATT CGTTTTCTCT ATGTTGGATT
     1201 CCTTGTTGCT CATATTGTGA TGATAATTTC TACAAATATA GTCATTGGTA ACTATCTATG
     1261 AAACTGTTTG ATACTTTTAT AGTTGATTAA ACTTGTTCAT GGCATTTGCC TTAATATCAT
     1321 CCGCTATGTC AATGTAGGGT TTCATAGCTT TGTAGTCGCT GTGTCCCGTC CATTTCATGA
     1381 CCACCTGTGC CGGGATTCCG AGAGCCAGCG CATTGCAGAT GAATGTCCTT CTTCCTGCAT
     1441 GGGTACTGAG CAAAGCGTAT TTGGGTGTGA CTTCATCAAT ACGTTCATTT CCCTTGTAGT
     1501 AGGTTTCCCG TACAGGCTCG TTGATTTCTG CCAGTTCGCC CAGCTCTTTC AGGTAATCGT
     1561 TCATCTTCTG GTTGCTGATG ACGGGCAGAG CCATGTAATT CTCGAAATGG ATGTCCTTGT
     1621 ATTTGTCCAG TATGGCTTTG CTGTATTTGT TCAGTTCAAT CGTCAGGCTG TCGGCAGTCT
     1681 TGACTGTGGT TATTTCGATG TGGTCGGACT TCACATCGCT TCTTTTCAGA TTGCGAACAT
     1741 CCGAATACCG CAAACTCGTA AAGCAGCAGA ACAGGAAAAC ATCACGCACA CGTTCCAGGT
     1801 ATTGCTTATC CTTGGGTATC TGGTAGTCTT TCAGCTTGTT CAGTTCATCC CAAGTCAGGA
     1861 AGATTACTTT TTTCGAGGTG GTTTTCAGTT TCGGTTTGAA CGTATCGTAT GCAATGTTCT
     1921 GATGATGTCC TTTCTTGAAG CTCCAGCGCA GGAACCATTT GAGGAATCCC ATTTGCTTGC
     1981 CGATGGTGCT GTTTCTCATA TCCTTGGTGT CACGCAGGAA GTTGACGTAT TCGTTCAATC
     2041 CAAACTCGTT GAAATAGTTG AACGTTGCAT CCTCCTTGAA CTCTTTGAGG TGGTTCCTCA
     2101 CTGCTGCAAA TTTTTCATAG GTGGATGCCG TCCAGTTATT CTGGTTACCG CACTCTTTTA
     2161 CAAACTCATC GAACACCTCC CAAAAGCTGA CAGGGGCTTC TTCCGGCTGT TCTTCGCTGG
     2221 TGTCTTTCAT TCTCATGTTG AAAGCTTCCT TCAACTGTTG GGTCGTTGGC ATGACCTCCT
     2281 GCACCTCAAA TTCCTTGAAA ATATTCTGGA TTTCGGCATA GTATTTCAGC AAGTCCGTAT
     2341 TGATTTCGGC TGCACTTTGC TTTAGCTTGT TGGTACATCC GCTCTTTACC CGCTGCTTAT
     2401 CTGCATCCCA TTTGGCTACG TCAATCCGGT AGCCCGTTGT AAACTCGATG CGTTGGCTGG
     2461 CAAAGATGAC ACGCATACGG ATGGGTACGT TCTCTACGAT TGGCACACCG TTCTTTTTCC
     2521 GGCTCTCCAA TGCAAAAATG ATGTTGCGCT TGATATTCAT AATTGGGTGC GTTTGAAATT
     2581 CTACACCCAA ATATACACCC AATTATTGAG ATAGCAAAAG ACATTTAGAA ACATTTACTT
     2641 TTACTCTATA TTGTAATTTA CACTTGATTA TCAGTCGTTT GCAGTCTTAT GATATTCTGT
     2701 GAAAGTATAA GTTCGAGAGC CTGTCTCTCC GCAAAAAACG CTGAAAATCA GCAGATTGCA
     2761 AAACAAACAC CCTGTTTTAC ACCCAAGAAT GTAAAGTCGG CTGTTTTTGT TTTATTTAAG
     2821 ATAATACAAC CACTACATAA TAAAAGAGTA GCGATATTAA AAGAATCCGA TGAGAAAAGA
     2881 CTAATATTTA TCTATCCATT CAGTTTGATT TTTCAGGACT TTACATCGTC CTGAAAGTAT
     2941 TTGTTGGTAC CGGTACCGAG GACGCGtaaa catttacagt tgcatgtggc ctattgtttT
     3001 TAGCCGTTAA ATATTTTATA ACTATTAAAT AGCGATACAA ATTGTTCGAA ACTAATATTG
     3061 TTTATATCAT ATATTCTCGC ATGTTTTAAA GCTTTATTAA ATTGATTTTT TGTAAACAGT
     3121 TTTTCGTACT CTTTGTTAAC CCATTTCATT ACAAAAGTTT CATATTTTTT TCTCTCTTTA
     3181 AATGCCATTT TTGCTGGCTT TCTTTTTAAT ACAATTAATG TGCTATCCAC TTTAGGTTTT
     3241 GGATGGAAAT AATACCTAGG AATTTTTGCT AATATAGAAA TATCTACCTC TGCCATTAAC
     3301 AGCAATGCTA GTGATCTGTT TGTATCTAAT AACATTTTAG CAAAACCATA TTCCACTATT
     3361 AAATAACTTA TTGTGGCTGA ACTTTCAAAA ACAATTTTTC GAATTATATT TGTGCTTATG
     3421 TTGTAAGGTA TGCTGCCAAA TATTTTATAT GGATTGTGGC TAGGAAATGT AAATTTCAGT
     3481 ATATCATCAT TTACTATTTG ATAGTTAGGA TAATTTAAGA GCTTATTACG AGTTACCTCA
     3541 CATAATTTAG AATCAATTTC TATCGCCGTT ACAAAATTAC ATCTCTTTAC CAATCCAGCA
     3601 GTAAAATGAC CTTTCCCTGC ACCTATTTCA AAGATGTTAT CTTTTTCATC TAAACTTATG
     3661 CAATTCATTA TTTTTTCTAT GTGATATTTT GAAGTAATAA AATTTTGACT ATCTTTTATA
     3721 TTTACTTTGT TCATTATAAC CTCTCCTTAA TTTATTGCAT CTCTTTTCGA ATATTTATGT
     3781 TTTTTGAGAA AAGAACGTAC TCATGGTTCA TCCCGATATG CGTATCGGTC TGTATATCAG
     3841 CAACTTTCTA TGTGTTTCAA CTACAATAGT CATCTATTCT CATCTTTCTG AGTCCACCCC
     3901 CTGCAAAGCC CCTCtttacg acataaaaat tcggtCGGAA AAGGTATGCA AAAGATGTTT
     3961 CTCTCTTTAA GAGAAACTCT TCGGGATGCA AAAATATGAA AATAACTCCA ATTCACCAAA
     4021 TTATATAGCG ACTTTTTTAC AAAATGCTAA AATTTGTTGA TTTCCGTCAA GCAATTGTTG
     4081 AGCAAAAATG TCTTTTACGA TAAAATGATA CCTCAATATC AACTGTTTAG CAAAACGATA
     4141 TTTCTCTTAA AGAGAGAAAC ACCTTTTTGT TCACCAATCC CCGACTTTTA ATCCCGCGGC
     4201 CATGATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT
     4261 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT
     4321 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG
     4381 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG
     4441 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA
     4501 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT
     4561 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC
     4621 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG
     4681 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC
     4741 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT
     4801 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA
     4861 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT
     4921 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC
     4981 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA
     5041 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC
     5101 TCATAACGCG T    
//
