LOCUS       pNBU2_erm_TetR_P        5907 bp ds-DNA     linear       31-MAR-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2076..3356
                     /label=NBU2 Integrase ORF
                     /ApEinfo_fwdcolor="#50ff41"
                     /ApEinfo_revcolor="#50ff41"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1498..1501)
                     /label=-33 binding site
                     /ApEinfo_fwdcolor="#ff764f"
                     /ApEinfo_revcolor="#ff764f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1714..1717
                     /label=-33 binding site(1)
                     /ApEinfo_label="-33 binding site"
                     /ApEinfo_fwdcolor="#ff764f"
                     /ApEinfo_revcolor="#ff764f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3516..3528
                     /label=attN2
                     /ApEinfo_fwdcolor="#ff786c"
                     /ApEinfo_revcolor="#ff786c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1739..1746
                     /label=-7 binding site
                     /ApEinfo_fwdcolor="#ff7e51"
                     /ApEinfo_revcolor="#ff7e51"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1464..1465)
                     /label=+1 txn intiation site
                     /ApEinfo_fwdcolor="#ffbd5c"
                     /ApEinfo_revcolor="#ffbd5c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1751..1751
                     /label=+1 txn initiation site
                     /ApEinfo_fwdcolor="#ffd544"
                     /ApEinfo_revcolor="#ffd544"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5018..5879
                     /label=bla ORF
                     /ApEinfo_fwdcolor="#6b92ff"
                     /ApEinfo_revcolor="#6b92ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1752..1770
                     /label=tetR binding site
                     /ApEinfo_fwdcolor="#ffc061"
                     /ApEinfo_revcolor="#ffc061"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1470..1477)
                     /label=-7 binding site(1)
                     /ApEinfo_label="-7 binding site"
                     /ApEinfo_fwdcolor="#ff511e"
                     /ApEinfo_revcolor="#ff511e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1802..1804
                     /label=start codon
                     /ApEinfo_fwdcolor="#b4ff9d"
                     /ApEinfo_revcolor="#b4ff9d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10..795
                     /label=RP4 oriT/R6K oriV
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1421..1463)
                     /label=A21 RBS
                     /ApEinfo_fwdcolor="#fd32ff"
                     /ApEinfo_revcolor="#fd32ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1992..2015
                     /label=3' sequencing primer
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(797..1420)
                     /label=tetR
                     /ApEinfo_fwdcolor="#fc1f14"
                     /ApEinfo_revcolor="#fc1f14"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1610..1630
                     /label=5' sequencing primer
                     /ApEinfo_fwdcolor="#f958ff"
                     /ApEinfo_revcolor="#f958ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3796..4530
                     /label=ermG ORF
                     /ApEinfo_fwdcolor="#82ff8f"
                     /ApEinfo_revcolor="#82ff8f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1690..1708)
                     /label=tetR binding site(1)
                     /ApEinfo_label="tetR binding site"
                     /ApEinfo_fwdcolor="#ffc061"
                     /ApEinfo_revcolor="#ffc061"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1772..1790
                     /label=GH023 ribosome binding site
                     /ApEinfo_fwdcolor="#97eaff"
                     /ApEinfo_revcolor="#97eaff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CAATTCGAGG GGGATCAATT CCGTGATAGG TGGGCTGCCC TTCCTGGTTG GCTTGGTTTC
       61 ATCAGCCATC CGCTTGCCCT CATCTGTTAC GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC
      121 AGGATTCCCG TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT
      181 CGCGGGTGGG CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA
      241 GTCTACACGA ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA
      301 AAAAATCGCT ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAACGGAA TTGATCCGGC
      361 CACGATGCGT CCGGCGTAGA GGATCTGAAG ATCAGCAGTT CAACCTGTTG ATAGTACGTA
      421 CTAAGCTCTC ATGTTTCACG TACTAAGCTC TCATGTTTAA CGTACTAAGC TCTCATGTTT
      481 AACGAACTAA ACCCTCATGG CTAACGTACT AAGCTCTCAT GGCTAACGTA CTAAGCTCTC
      541 ATGTTTCACG TACTAAGCTC TCATGTTTGA ACAATAAAAT TAATATAAAT CAGCAACTTA
      601 AATAGCCTCT AAGGTTTTAA GTTTTATAAG AAAAAAAAGA ATATATAAGG CTTTTAAAGC
      661 TTTTAAGGTT TAACGGTTGT GGACaacaag ccagggatgt aacgCACTGA GAAGCCCTTA
      721 GAGCCTCTCA AAGCAATTTT GAGTGACACA GGAACACTTA ACGGCTGACA TGGGAATTCC
      781 CCTCCACCGC GGTGGCTTAA GACCCACTTT CACATTTAAG TTGTTTTTCT AATCCGCATA
      841 TGATCAATTC AAGGCCGAAT AAGAAGGCTG GCTCTGCACC TTGGTGATCA AATAATTCGA
      901 TAGCTTGTCG TAATAATGGC GGCATACTAT CAGTAGTAGG TGTTTCCCTT TCTTCTTTAG
      961 CGACTTGATG CTCTTGATCT TCCAATACGC AACCTAAAGT AAAATGCCCC ACAGCGCTGA
     1021 GTGCATATAA TGCATTCTCT AGTGAAAAAC CTTGTTGGCA TAAAAAGGCT AATTGATTTT
     1081 CGAGAGTTTC ATACTGTTTT TCTGTAGGCC GTGTACCTAA ATGTACTTTT GCTCCATCGC
     1141 GATGACTTAG TAAAGCACAT CTAAAACTTT TAGCGTTATT ACGTAAAAAA TCTTGCCAGC
     1201 TTTCCCCTTC TAAAGGGCAA AAGTGAGTAT GGTGCCTATC TAACATCTCA ATGGCTAAGG
     1261 CGTCGAGCAA AGCCCGCTTA TTTTTTACAT GCCAATACAA TGTAGGCTGC TCTACACCTA
     1321 GCTTCTGGGC GAGTTTACGG GTTGTTAAAC CTTCGATTCC GACCTCATTA AGCAGCTCTA
     1381 ATGCGCTGTT AATCACTTTA CTTTTATCTA ATCTAGACAT attcgtttaa tatcataaat
     1441 aatttatttt attttaaaat gcgCGggtgC AAAGGTAaga ggttttattt taactacCAA
     1501 Atgttttcgg aagttttttc gcttttcttt ttctatcgtt tctcagactc tcttagcgaa
     1561 agggaaagaa ggtaaagaag aaaaacaaaa cgccttttct tTtttgcacc cgctttccaa
     1621 gagaagaaag ccttgttaaa ttgacttagt gtaaaagcgc agtactgctt gaccataaga
     1681 acaaaaaaaT CTCTATCACT GATAGGGAta aagTTTGgaa gataaagcta aaagttctTA
     1741 TCTTTGcagt CTCCCTATCA GTGATAGAGA cGAAATAAAG ACATATAAAA gaaaagacac
     1801 cATGGTGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC TTATCGATAC CGTCGACGTC
     1861 GACTAATTGC CTATCTTCCA GTGATGGAAC AGCATTTGTG CATTGGCTGC AACAATCAGC
     1921 CTTACTTGTG CCTGTTCTAT TTCCGAACCG ACCGCTTGTA TGAATCCATC AAAATTCGTT
     1981 TTCTCTATGT TGGATTCCTT GTTGCTCATA TTGTGATGAT AATTTCTACA AATATAGTCA
     2041 TTGGTAACTA TCTATGAAAC TGTTTGATAC TTTTATAGTT GATTAAACTT GTTCATGGCA
     2101 TTTGCCTTAA TATCATCCGC TATGTCAATG TAGGGTTTCA TAGCTTTGTA GTCGCTGTGT
     2161 CCCGTCCATT TCATGACCAC CTGTGCCGGG ATTCCGAGAG CCAGCGCATT GCAGATGAAT
     2221 GTCCTTCTTC CTGCATGGGT ACTGAGCAAA GCGTATTTGG GTGTGACTTC ATCAATACGT
     2281 TCATTTCCCT TGTAGTAGGT TTCCCGTACA GGCTCGTTGA TTTCTGCCAG TTCGCCCAGC
     2341 TCTTTCAGGT AATCGTTCAT CTTCTGGTTG CTGATGACGG GCAGAGCCAT GTAATTCTCG
     2401 AAATGGATGT CCTTGTATTT GTCCAGTATG GCTTTGCTGT ATTTGTTCAG TTCAATCGTC
     2461 AGGCTGTCGG CAGTCTTGAC TGTGGTTATT TCGATGTGGT CGGACTTCAC ATCGCTTCTT
     2521 TTCAGATTGC GAACATCCGA ATACCGCAAA CTCGTAAAGC AGCAGAACAG GAAAACATCA
     2581 CGCACACGTT CCAGGTATTG CTTATCCTTG GGTATCTGGT AGTCTTTCAG CTTGTTCAGT
     2641 TCATCCCAAG TCAGGAAGAT TACTTTTTTC GAGGTGGTTT TCAGTTTCGG TTTGAACGTA
     2701 TCGTATGCAA TGTTCTGATG ATGTCCTTTC TTGAAGCTCC AGCGCAGGAA CCATTTGAGG
     2761 AATCCCATTT GCTTGCCGAT GGTGCTGTTT CTCATATCCT TGGTGTCACG CAGGAAGTTG
     2821 ACGTATTCGT TCAATCCAAA CTCGTTGAAA TAGTTGAACG TTGCATCCTC CTTGAACTCT
     2881 TTGAGGTGGT TCCTCACTGC TGCAAATTTT TCATAGGTGG ATGCCGTCCA GTTATTCTGG
     2941 TTACCGCACT CTTTTACAAA CTCATCGAAC ACCTCCCAAA AGCTGACAGG GGCTTCTTCC
     3001 GGCTGTTCTT CGCTGGTGTC TTTCATTCTC ATGTTGAAAG CTTCCTTCAA CTGTTGGGTC
     3061 GTTGGCATGA CCTCCTGCAC CTCAAATTCC TTGAAAATAT TCTGGATTTC GGCATAGTAT
     3121 TTCAGCAAGT CCGTATTGAT TTCGGCTGCA CTTTGCTTTA GCTTGTTGGT ACATCCGCTC
     3181 TTTACCCGCT GCTTATCTGC ATCCCATTTG GCTACGTCAA TCCGGTAGCC CGTTGTAAAC
     3241 TCGATGCGTT GGCTGGCAAA GATGACACGC ATACGGATGG GTACGTTCTC TACGATTGGC
     3301 ACACCGTTCT TTTTCCGGCT CTCCAATGCA AAAATGATGT TGCGCTTGAT ATTCATAATT
     3361 GGGTGCGTTT GAAATTCTAC ACCCAAATAT ACACCCAATT ATTGAGATAG CAAAAGACAT
     3421 TTAGAAACAT TTACTTTTAC TCTATATTGT AATTTACACT TGATTATCAG TCGTTTGCAG
     3481 TCTTATGATA TTCTGTGAAA GTATAAGTTC GAGAGCCTGT CTCTCCGCAA AAAACGCTGA
     3541 AAATCAGCAG ATTGCAAAAC AAACACCCTG TTTTACACCC AAGAATGTAA AGTCGGCTGT
     3601 TTTTGTTTTA TTTAAGATAA TACAACCACT ACATAATAAA AGAGTAGCGA TATTAAAAGA
     3661 ATCCGATGAG AAAAGACTAA TATTTATCTA TCCATTCAGT TTGATTTTTC AGGACTTTAC
     3721 ATCGTCCTGA AAGTATTTGT TGGTACCGGT ACCGAGGACG CGtaaacatt tacagttgca
     3781 tgtggcctat tgtttTTAGC CGTTAAATAT TTTATAACTA TTAAATAGCG ATACAAATTG
     3841 TTCGAAACTA ATATTGTTTA TATCATATAT TCTCGCATGT TTTAAAGCTT TATTAAATTG
     3901 ATTTTTTGTA AACAGTTTTT CGTACTCTTT GTTAACCCAT TTCATTACAA AAGTTTCATA
     3961 TTTTTTTCTC TCTTTAAATG CCATTTTTGC TGGCTTTCTT TTTAATACAA TTAATGTGCT
     4021 ATCCACTTTA GGTTTTGGAT GGAAATAATA CCTAGGAATT TTTGCTAATA TAGAAATATC
     4081 TACCTCTGCC ATTAACAGCA ATGCTAGTGA TCTGTTTGTA TCTAATAACA TTTTAGCAAA
     4141 ACCATATTCC ACTATTAAAT AACTTATTGT GGCTGAACTT TCAAAAACAA TTTTTCGAAT
     4201 TATATTTGTG CTTATGTTGT AAGGTATGCT GCCAAATATT TTATATGGAT TGTGGCTAGG
     4261 AAATGTAAAT TTCAGTATAT CATCATTTAC TATTTGATAG TTAGGATAAT TTAAGAGCTT
     4321 ATTACGAGTT ACCTCACATA ATTTAGAATC AATTTCTATC GCCGTTACAA AATTACATCT
     4381 CTTTACCAAT CCAGCAGTAA AATGACCTTT CCCTGCACCT ATTTCAAAGA TGTTATCTTT
     4441 TTCATCTAAA CTTATGCAAT TCATTATTTT TTCTATGTGA TATTTTGAAG TAATAAAATT
     4501 TTGACTATCT TTTATATTTA CTTTGTTCAT TATAACCTCT CCTTAATTTA TTGCATCTCT
     4561 TTTCGAATAT TTATGTTTTT TGAGAAAAGA ACGTACTCAT GGTTCATCCC GATATGCGTA
     4621 TCGGTCTGTA TATCAGCAAC TTTCTATGTG TTTCAACTAC AATAGTCATC TATTCTCATC
     4681 TTTCTGAGTC CACCCCCTGC AAAGCCCCTC tttacgacat aaaaattcgg tCGGAAAAGG
     4741 TATGCAAAAG ATGTTTCTCT CTTTAAGAGA AACTCTTCGG GATGCAAAAA TATGAAAATA
     4801 ACTCCAATTC ACCAAATTAT ATAGCGACTT TTTTACAAAA TGCTAAAATT TGTTGATTTC
     4861 CGTCAAGCAA TTGTTGAGCA AAAATGTCTT TTACGATAAA ATGATACCTC AATATCAACT
     4921 GTTTAGCAAA ACGATATTTC TCTTAAAGAG AGAAACACCT TTTTGTTCAC CAATCCCCGA
     4981 CTTTTAATCC CGCGGCCATG ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
     5041 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
     5101 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
     5161 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
     5221 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
     5281 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
     5341 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
     5401 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
     5461 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
     5521 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT
     5581 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
     5641 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
     5701 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG
     5761 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
     5821 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT
     5881 GTCAGACCAA GTTTACTCAT AACGCGT   
//
