LOCUS       Exported                7507 bp ds-DNA     circular SYN 03-APR-2017
DEFINITION  pLKO5d.SFFV.eGFP.miR30n rotated to position 5796.
ACCESSION   .
VERSION     .
KEYWORDS    pLKO5d.SFFV.eGFP.miR30n
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 3, 2017 from SnapGene Viewer 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..414
                     /note="RSV/5'LTR"
                     /note="LTR"
     misc_feature    465..602
                     /note="psi"
     misc_feature    569..933
                     /note="gag"
     misc_feature    1079..1320
                     /note="RRE"
     misc_feature    1753..1870
                     /note="cPPT"
     misc_feature    1917..2329
                     /note="SFFV"
     misc_feature    2341..3060
                     /note="eGFP"
     misc_feature    3061..3192
                     /note="miR30 5p arm"
     misc_feature    3193..3219
                     /note="miR30"
     misc_feature    3193..3195
                     /note="pre-mir"
     misc_feature    3217..3219
                     /note="pre-mir"
     misc_feature    3220..3350
                     /note="miR30 3p arm"
     misc_feature    3365..3953
                     /note="WPRE"
                     /note="misc_RNA"
     misc_feature    4024..4259
                     /note="3'\LTR\SIN"
                     /note="LTR"
     misc_feature    4336..4466
                     /note="SV40\polyA"
                     /note="polyA_signal"
     misc_feature    4813..5243
                     /note="f1\Ori"
                     /note="rep_origin"
     misc_feature    5614..6420
                     /note="AmpR"
     misc_feature    complement(6546..7161)
                     /note="pUC\Ori"
                     /note="rep_origin"
ORIGIN
        1 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acaattctag atcttgagac aaatggcagt
     1741 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1801 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     1861 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgagcg
     1921 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
     1981 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
     2041 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     2101 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     2161 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     2221 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     2281 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgg atccgccacc
     2341 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2401 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2461 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2521 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2581 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2641 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2701 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2761 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2821 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2881 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2941 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3001 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga
     3061 atattgctgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa
     3121 acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctaaagaa ggtatattgc
     3181 tgttgacagt gagcgggaga cgtgattacc gtctcttgcc tactgcctcg gacttcaagg
     3241 ggctacttta ggagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat
     3301 acatttttac aaagctgaat taaaatggta taaattaaat cactttttta cgcgttaagt
     3361 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3421 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3481 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3541 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3601 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3661 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3721 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3781 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3841 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3901 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     3961 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4021 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     4081 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4141 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4201 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     4261 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     4321 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4441 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     4501 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4561 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4621 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     4681 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4741 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4801 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4861 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4921 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4981 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5041 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5101 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5161 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5221 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5281 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5341 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5401 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5461 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5521 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5581 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5641 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5701 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5761 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5821 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5881 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5941 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6001 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6061 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6121 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6181 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6241 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6301 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6361 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6421 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6481 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6541 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6601 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6661 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6721 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6781 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6841 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6901 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6961 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7021 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7081 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7141 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7201 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7261 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7321 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7381 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7441 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     7501 ctgcaag
//
