LOCUS       Exported                9293 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2085
                     /label=NFkB
                     /note="NFkB"
     primer_bind     2086..2105
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2129..2159
                     /label=minP
                     /note="minP"
     TATA_signal     2135..2138
                     /label=TATA
                     /note="TATA"
     gene            2192..3967
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3751..3765
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3999..4015
                     /estimated_length=17
     unsure          3999..4015
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     4016..4032
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    4048..4645
                     /label=WPRE
                     /note="WPRE"
     promoter        4656..4964
                     /label=SV40
                     /note="SV40"
     gene            5023..5742
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5813..5866
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5814..6047
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6119..6250
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7109..7207
                     /label=bla promoter
                     /note="bla promoter"
     gene            7208..8068
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8213..8886
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcctagt ggggactttc cacctgggga ctttccacct
     1981 ctagtgggga ctttccacct ggggactttc cacctctagt ggggactttc cacctgggga
     2041 ctttccacct ctagtgggga ctttccacct ggggactttc cacctgatcc gagaacagat
     2101 ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc gacttccagc
     2161 ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg
     2221 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
     2281 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     2341 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
     2401 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     2461 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     2521 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     2581 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     2641 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     2701 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     2761 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     2821 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     2881 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     2941 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     3001 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     3061 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     3121 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     3181 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     3241 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     3301 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     3361 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
     3421 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     3481 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     3541 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     3601 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     3661 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     3721 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     3781 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     3841 gaattctcac ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc tgcccatgag
     3901 ctgcgcccag gagagcggca tggatagaca ccctgctgct tgcgccagcg ccaggatcaa
     3961 cgtctaaggc cgcgactcta gaacgcgtca ctgcggctnn nnnnnnnnnn nnnnngacta
     4021 cagacatcag cgaccggtta gtaatgatcg acaatcaacc tctggattac aaaatttgtg
     4081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4201 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4261 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4321 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4381 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4441 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4501 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4561 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4621 ccctttgggc cgcctccccg cctggcgatg gtaccggtgt ggaaagtccc caggctcccc
     4681 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4741 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4801 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4861 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4921 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4981 gggagcttgt atatccattt tcggatctga tcagcaccta gcatggtgag caagggcgag
     5041 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5101 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5161 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc
     5221 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     5281 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5341 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5401 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5461 aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc
     5521 aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5581 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     5641 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5701 gccgccggga tcactctcgg catggacgag ctgtacaagt aaggtacctt taagaccaat
     5761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5821 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     6061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     6121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6241 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6421 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6601 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6661 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6721 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6781 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6841 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6901 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6961 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7021 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7081 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7141 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7201 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7261 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7321 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7381 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7441 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7501 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7561 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7621 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7681 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7741 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7801 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7861 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7921 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7981 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8041 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8101 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8161 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8221 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8281 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8341 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8401 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8461 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8521 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8581 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8641 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8701 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8761 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8821 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8881 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8941 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9001 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9061 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9241 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
