LOCUS       Exported                9288 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2080
                     /label=ATF6
                     /note="ATF6"
     primer_bind     2081..2100
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2124..2154
                     /label=minP
                     /note="minP"
     TATA_signal     2130..2133
                     /label=TATA
                     /note="TATA"
     gene            2187..3962
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3746..3760
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3994..4010
                     /estimated_length=17
     unsure          3994..4010
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     4011..4027
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    4043..4640
                     /label=WPRE
                     /note="WPRE"
     promoter        4651..4959
                     /label=SV40
                     /note="SV40"
     gene            5018..5737
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5808..5861
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5809..6042
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6114..6245
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7104..7202
                     /label=bla promoter
                     /note="bla promoter"
     gene            7203..8063
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8208..8881
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcctcga gacaggtgct gacgtggcat tcctcgagac
     1981 aggtgctgac gtggcattcc tcgagacagg tgctgacgtg gcattcctcg agacaggtgc
     2041 tgacgtggca ttcctcgaga caggtgctga cgtggcattc gatccgagaa cagatctggc
     2101 ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt ccagcttggc
     2161 aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag
     2221 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
     2281 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
     2341 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
     2401 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
     2461 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
     2521 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
     2581 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
     2641 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
     2701 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     2761 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     2821 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     2881 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     2941 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     3001 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     3061 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     3121 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     3181 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     3241 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     3301 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     3361 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     3421 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     3481 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     3541 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     3601 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     3661 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     3721 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     3781 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaatt
     3841 ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc atgagctgcg
     3901 cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg atcaacgtct
     3961 aaggccgcga ctctagaacg cgtcactgcg gctnnnnnnn nnnnnnnnnn gactacagac
     4021 atcagcgacc ggttagtaat gatcgacaat caacctctgg attacaaaat ttgtgaaaga
     4081 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4141 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4201 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4261 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4321 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4381 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4441 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4501 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4561 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4621 tgggccgcct ccccgcctgg cgatggtacc ggtgtggaaa gtccccaggc tccccagcag
     4681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4741 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4981 cttgtatatc cattttcgga tctgatcagc acctagcatg gtgagcaagg gcgaggagct
     5041 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5101 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5161 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     5221 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5281 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5341 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5401 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     5461 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
     5521 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     5581 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     5641 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5701 cgggatcact ctcggcatgg acgagctgta caagtaaggt acctttaaga ccaatgactt
     5761 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5821 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5881 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5941 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6001 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6061 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6241 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6301 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6361 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6421 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6481 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6541 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6601 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6661 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6721 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6781 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6841 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6901 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6961 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7021 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7081 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7141 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7201 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7261 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7321 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7381 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7441 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7501 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7561 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7621 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7681 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7741 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7801 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7861 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7921 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7981 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8041 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8341 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8881 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8941 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9001 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9061 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9181 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9241 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagctt
//
