LOCUS       Exported                9303 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2095
                     /label=STAT5
                     /note="STAT5"
     primer_bind     2096..2115
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2139..2169
                     /label=minP
                     /note="minP"
     TATA_signal     2145..2148
                     /label=TATA
                     /note="TATA"
     gene            2202..3977
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3761..3775
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             4009..4025
                     /estimated_length=17
     unsure          4009..4025
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     4026..4042
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    4058..4655
                     /label=WPRE
                     /note="WPRE"
     promoter        4666..4974
                     /label=SV40
                     /note="SV40"
     gene            5033..5752
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5823..5876
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5824..6057
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6129..6260
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7119..7217
                     /label=bla promoter
                     /note="bla promoter"
     gene            7218..8078
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8223..8896
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgcttt tagatttcta ggaattcaat ccttttagat
     1981 ttctaggaat tcaatccttt agatttctag gaattcaatc cttttagatt tctaggaatt
     2041 caatccttta gatttctagg aattcaatcc ttttagattt ctaggaattc aatccgatcc
     2101 gagaacagat ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc
     2161 gacttccagc ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca
     2221 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     2281 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
     2341 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     2401 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
     2461 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     2521 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     2581 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     2641 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     2701 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     2761 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     2821 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     2881 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     2941 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     3001 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     3061 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     3121 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     3181 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     3241 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     3301 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     3361 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     3421 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     3481 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     3541 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     3601 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     3661 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     3721 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     3781 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     3841 agatcgccgt gaattctcac ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc
     3901 tgcccatgag ctgcgcccag gagagcggca tggatagaca ccctgctgct tgcgccagcg
     3961 ccaggatcaa cgtctaaggc cgcgactcta gaacgcgtca ctgcggctnn nnnnnnnnnn
     4021 nnnnngacta cagacatcag cgaccggtta gtaatgatcg acaatcaacc tctggattac
     4081 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4141 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4201 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4261 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4321 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4381 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4441 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     4501 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4561 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4621 agtcggatct ccctttgggc cgcctccccg cctggcgatg gtaccggtgt ggaaagtccc
     4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4801 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4861 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4921 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4981 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcaccta gcatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaggtacctt
     5761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5821 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6421 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6601 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7081 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9241 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9301 ctt
//
