LOCUS       Exported                9235 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2027
                     /label=FOXO
                     /note="FOXO"
     primer_bind     2028..2047
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2071..2101
                     /label=minP
                     /note="minP"
     TATA_signal     2077..2080
                     /label=TATA
                     /note="TATA"
     gene            2134..3909
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3693..3707
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3941..3957
                     /estimated_length=17
     unsure          3941..3957
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3958..3974
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3990..4587
                     /label=WPRE
                     /note="WPRE"
     promoter        4598..4906
                     /label=SV40
                     /note="SV40"
     gene            4965..5684
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5755..5808
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5756..5989
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6061..6192
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7051..7149
                     /label=bla promoter
                     /note="bla promoter"
     gene            7150..8010
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8155..8828
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctcgaa agtaaacaac tatgtaaaca actataagta
     1981 aacaactatg taaacaacta taagtaaaca actatgtaaa caagatcgat ccgagaacag
     2041 atctggcctc ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca
     2101 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
     2161 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     2221 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     2281 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     2341 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     2401 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     2461 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     2521 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     2581 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     2641 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     2701 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     2761 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     2821 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     2881 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     2941 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     3001 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     3061 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     3121 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     3181 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     3241 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     3301 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     3361 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     3421 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     3481 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     3541 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     3601 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     3661 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     3721 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     3781 gtgaattctc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg
     3841 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc
     3901 aacgtctaag gccgcgactc tagaacgcgt cactgcggct nnnnnnnnnn nnnnnnngac
     3961 tacagacatc agcgaccggt tagtaatgat cgacaatcaa cctctggatt acaaaatttg
     4021 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4081 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4141 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4201 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4261 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4321 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4381 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     4441 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4501 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4561 ctccctttgg gccgcctccc cgcctggcga tggtaccggt gtggaaagtc cccaggctcc
     4621 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4681 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4741 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4801 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4861 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4921 ccgggagctt gtatatccat tttcggatct gatcagcacc tagcatggtg agcaagggcg
     4981 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     5041 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     5101 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca
     5161 cctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     5221 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     5281 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     5341 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     5401 acaacagcca caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact
     5461 tcaagacccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     5521 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     5581 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     5641 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaggtacc tttaagacca
     5701 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5761 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5821 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5881 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5941 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6001 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6061 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6121 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6181 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6241 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6301 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6361 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     6421 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6481 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6541 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6601 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6661 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6721 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6781 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6841 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6901 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6961 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     7021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8881 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8941 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9001 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9061 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9181 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agctt
//
