LOCUS       Exported                9229 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2021
                     /label=HIF1a
                     /note="HIF1a"
     primer_bind     2022..2041
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2065..2095
                     /label=minP
                     /note="minP"
     TATA_signal     2071..2074
                     /label=TATA
                     /note="TATA"
     gene            2128..3903
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3687..3701
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3935..3951
                     /estimated_length=17
     unsure          3935..3951
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3952..3968
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3984..4581
                     /label=WPRE
                     /note="WPRE"
     promoter        4592..4900
                     /label=SV40
                     /note="SV40"
     gene            4959..5678
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5749..5802
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5750..5983
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6055..6186
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7045..7143
                     /label=bla promoter
                     /note="bla promoter"
     gene            7144..8004
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8149..8822
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgtgac tacgtgctgc ctaggtgact acgtgctgcc
     1981 taggtgacta cgtgctgcct aggtgactac gtgctgccta ggatccgaga acagatctgg
     2041 cctcggcggc caagcttaga cactagaggg tatataatgg aagctcgact tccagcttgg
     2101 caatccggta ctgttggtaa agccaccatg gaagatgcca aaaacattaa gaagggccca
     2161 gcgccattct acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag
     2221 cgctacgccc tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt
     2281 acctacgccg agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg
     2341 ctgaatacaa accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc
     2401 gtgttgggtg ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag
     2461 cgcgagctgc tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa
     2521 gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc
     2581 atggatagca agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat
     2641 ttgccacccg gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc
     2701 atcgccctga tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg
     2761 caccgcaccg cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc
     2821 atccccgaca ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc
     2881 acgctgggct acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag
     2941 ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta
     3001 tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag
     3061 atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc
     3121 cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc
     3181 acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct
     3241 aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc
     3301 gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc
     3361 atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac
     3421 ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca
     3481 gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc
     3541 ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa
     3601 accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag
     3661 ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac
     3721 gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgaat
     3781 tctcacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc catgagctgc
     3841 gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag gatcaacgtc
     3901 taaggccgcg actctagaac gcgtcactgc ggctnnnnnn nnnnnnnnnn ngactacaga
     3961 catcagcgac cggttagtaa tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag
     4021 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4081 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4141 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4201 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4261 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4321 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4381 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4441 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4501 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4561 ttgggccgcc tccccgcctg gcgatggtac cggtgtggaa agtccccagg ctccccagca
     4621 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4681 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4741 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4801 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4861 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4921 gcttgtatat ccattttcgg atctgatcag cacctagcat ggtgagcaag ggcgaggagc
     4981 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5041 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5101 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg
     5161 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5221 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     5281 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5341 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     5401 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     5461 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     5521 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     5581 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     5641 ccgggatcac tctcggcatg gacgagctgt acaagtaagg tacctttaag accaatgact
     5701 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5761 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5821 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5881 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5941 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6001 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6061 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6121 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6181 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6241 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6301 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6361 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6421 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6481 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6541 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6601 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6781 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6901 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7021 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7081 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7141 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7201 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7261 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7321 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7381 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7441 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7501 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7561 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7621 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7681 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7741 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7801 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7861 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7921 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7981 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8041 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8281 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8881 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8941 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9001 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9061 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9121 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9181 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
