LOCUS       Exported                9219 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2011
                     /label=SRF
                     /note="SRF"
     primer_bind     2012..2031
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2055..2085
                     /label=minP
                     /note="minP"
     TATA_signal     2061..2064
                     /label=TATA
                     /note="TATA"
     gene            2118..3893
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3677..3691
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3925..3941
                     /estimated_length=17
     unsure          3925..3941
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3942..3958
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3974..4571
                     /label=WPRE
                     /note="WPRE"
     promoter        4582..4890
                     /label=SV40
                     /note="SV40"
     gene            4949..5668
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5739..5792
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5740..5973
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6045..6176
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7035..7133
                     /label=bla promoter
                     /note="bla promoter"
     gene            7134..7994
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8139..8812
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcggatg tccatattag gacatctgga tgtccatatt
     1981 aggacatctg gatgtccata ttaggacatc tgatccgaga acagatctgg cctcggcggc
     2041 caagcttaga cactagaggg tatataatgg aagctcgact tccagcttgg caatccggta
     2101 ctgttggtaa agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct
     2161 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
     2221 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     2281 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     2341 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
     2401 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     2461 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     2521 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     2581 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     2641 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     2701 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     2761 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     2821 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     2881 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     2941 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     3001 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     3061 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     3121 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     3181 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     3241 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     3301 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     3361 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     3421 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     3481 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     3541 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     3601 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     3661 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     3721 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgaat tctcacggct
     3781 tccctcccga ggtggaggag caggccgccg gcaccctgcc catgagctgc gcccaggaga
     3841 gcggcatgga tagacaccct gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg
     3901 actctagaac gcgtcactgc ggctnnnnnn nnnnnnnnnn ngactacaga catcagcgac
     3961 cggttagtaa tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4381 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4501 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4561 tccccgcctg gcgatggtac cggtgtggaa agtccccagg ctccccagca ggcagaagta
     4621 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4741 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4801 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     4861 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     4921 ccattttcgg atctgatcag cacctagcat ggtgagcaag ggcgaggagc tgttcaccgg
     4981 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5041 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5101 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg
     5161 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5221 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5281 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5341 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
     5401 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
     5461 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5521 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5581 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5641 tctcggcatg gacgagctgt acaagtaagg tacctttaag accaatgact tacaaggcag
     5701 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5761 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5941 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6001 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6061 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6121 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6181 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6301 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6361 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6541 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6601 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6661 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6721 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6781 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6841 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6901 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6961 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7021 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7081 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7141 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7201 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7261 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7321 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7381 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7441 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7501 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7561 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7621 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7681 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7741 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7801 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7861 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7921 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7981 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8041 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8101 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8161 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8221 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8281 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8341 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8401 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8461 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8521 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8581 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8641 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8701 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8761 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8821 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8881 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8941 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9001 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9061 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9121 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     9181 accctcacta aagggaacaa aagctggagc tgcaagctt
//
