LOCUS       Exported                9243 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2035
                     /label=NFAT
                     /note="NFAT"
     primer_bind     2036..2055
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2079..2109
                     /label=minP
                     /note="minP"
     TATA_signal     2085..2088
                     /label=TATA
                     /note="TATA"
     gene            2142..3917
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3701..3715
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3949..3965
                     /estimated_length=17
     unsure          3949..3965
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3966..3982
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3998..4595
                     /label=WPRE
                     /note="WPRE"
     promoter        4606..4914
                     /label=SV40
                     /note="SV40"
     gene            4973..5692
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5763..5816
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5764..5997
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6069..6200
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7059..7157
                     /label=bla promoter
                     /note="bla promoter"
     gene            7158..8018
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8163..8836
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcggagg aaaaactgtt tcatacagaa ggcgtggagg
     1981 aaaaactgtt tcatacagaa ggcgtggagg aaaaactgtt tcatacagaa ggcgtgatcc
     2041 gagaacagat ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc
     2101 gacttccagc ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca
     2161 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     2221 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
     2281 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     2341 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
     2401 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     2461 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     2521 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     2581 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     2641 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     2701 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     2761 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     2821 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     2881 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     2941 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     3001 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     3061 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     3121 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     3181 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     3241 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     3301 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     3361 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     3421 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     3481 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     3541 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     3601 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     3661 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     3721 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     3781 agatcgccgt gaattctcac ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc
     3841 tgcccatgag ctgcgcccag gagagcggca tggatagaca ccctgctgct tgcgccagcg
     3901 ccaggatcaa cgtctaaggc cgcgactcta gaacgcgtca ctgcggctnn nnnnnnnnnn
     3961 nnnnngacta cagacatcag cgaccggtta gtaatgatcg acaatcaacc tctggattac
     4021 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4081 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4141 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4201 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4261 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4321 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4381 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     4441 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4501 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4561 agtcggatct ccctttgggc cgcctccccg cctggcgatg gtaccggtgt ggaaagtccc
     4621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4861 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4921 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcaccta gcatggtgag
     4981 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5041 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5101 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5161 caccttcacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5221 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5281 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5341 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5401 gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa
     5461 ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5521 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5581 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5641 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaggtacctt
     5701 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5761 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5821 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5881 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5941 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6001 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6061 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6121 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6181 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6301 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6361 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6421 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6481 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6541 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7021 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7081 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7141 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7201 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7261 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7321 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7381 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7441 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7501 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7561 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7621 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7681 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7741 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7801 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7861 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7921 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7981 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8041 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8101 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8161 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8221 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8281 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8341 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8401 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8461 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8521 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8581 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8641 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8701 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8761 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8821 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8881 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8941 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9001 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9061 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9121 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9181 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9241 ctt
//
