LOCUS       Exported                9202 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..1994
                     /label=XBP1
                     /note="XBP1"
     primer_bind     1995..2014
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2038..2068
                     /label=minP
                     /note="minP"
     TATA_signal     2044..2047
                     /label=TATA
                     /note="TATA"
     gene            2101..3876
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3660..3674
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3908..3924
                     /estimated_length=17
     unsure          3908..3924
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3925..3941
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3957..4554
                     /label=WPRE
                     /note="WPRE"
     promoter        4565..4873
                     /label=SV40
                     /note="SV40"
     gene            4932..5651
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5722..5775
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5723..5956
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6028..6159
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7018..7116
                     /label=bla promoter
                     /note="bla promoter"
     gene            7117..7977
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8122..8795
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatccaaca gatgcgtcat gcgtcatgcg tcatgcgtca
     1981 tgcgtcatct caaagatccg agaacagatc tggcctcggc ggccaagctt agacactaga
     2041 gggtatataa tggaagctcg acttccagct tggcaatccg gtactgttgg taaagccacc
     2101 atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg
     2161 accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc
     2221 gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc
     2281 gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg
     2341 tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg
     2401 gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc
     2461 agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa
     2521 aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc
     2581 ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa cgagtacgac
     2641 ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggc
     2701 agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt
     2761 catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat cctcagcgtg
     2821 gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt
     2881 cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat
     2941 aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc
     3001 atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc
     3061 aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac
     3121 ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc
     3181 gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag
     3241 acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc
     3301 tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc
     3361 ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc
     3421 ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa
     3481 caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg
     3541 cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac
     3601 tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac
     3661 gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt
     3721 aaggccaaga agggcggcaa gatcgccgtg aattctcacg gcttccctcc cgaggtggag
     3781 gagcaggccg ccggcaccct gcccatgagc tgcgcccagg agagcggcat ggatagacac
     3841 cctgctgctt gcgccagcgc caggatcaac gtctaaggcc gcgactctag aacgcgtcac
     3901 tgcggctnnn nnnnnnnnnn nnnngactac agacatcagc gaccggttag taatgatcga
     3961 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4021 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4081 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4141 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4201 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4261 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4321 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     4381 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4441 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4501 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcgatgg
     4561 taccggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4621 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4681 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4741 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4801 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4861 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4921 cagcacctag catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4981 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5041 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5101 cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcgcc cgctaccccg
     5161 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5221 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5281 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5341 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca
     5401 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg
     5461 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5521 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5581 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5641 tgtacaagta aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5701 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     5761 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5821 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5881 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5941 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6001 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6061 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6121 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6181 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6241 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6301 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6361 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6421 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6481 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6541 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6601 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6661 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6721 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6781 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6841 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6901 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6961 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     7021 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7081 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7141 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7321 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7621 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7681 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7741 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7801 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7861 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7921 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7981 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8041 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8101 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8161 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8221 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8281 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8341 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8401 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8461 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8521 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8581 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8641 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8701 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8761 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8821 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8881 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8941 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     9001 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9061 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9121 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     9181 caaaagctgg agctgcaagc tt
//
