LOCUS       Exported                9211 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2003
                     /label=p53
                     /note="p53"
     primer_bind     2004..2023
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2047..2077
                     /label=minP
                     /note="minP"
     TATA_signal     2053..2056
                     /label=TATA
                     /note="TATA"
     gene            2110..3885
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3669..3683
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3917..3933
                     /estimated_length=17
     unsure          3917..3933
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3934..3950
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3966..4563
                     /label=WPRE
                     /note="WPRE"
     promoter        4574..4882
                     /label=SV40
                     /note="SV40"
     gene            4941..5660
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5731..5784
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5732..5965
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6037..6168
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7027..7125
                     /label=bla promoter
                     /note="bla promoter"
     gene            7126..7986
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8131..8804
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctacag aacatgtcta agcatgctgt gccttgcctg
     1981 gacttgcctg gccttgcctt ggggatccga gaacagatct ggcctcggcg gccaagctta
     2041 gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt
     2101 aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc
     2161 gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc
     2221 ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc
     2281 gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg
     2341 atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc
     2401 atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc
     2461 atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc
     2521 aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac
     2581 taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac
     2641 gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac
     2701 agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc
     2761 cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc
     2821 ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc
     2881 tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg
     2941 caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag
     3001 agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg
     3061 ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc
     3121 cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac
     3181 aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac
     3241 accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc
     3301 atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg
     3361 ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg
     3421 ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc
     3481 ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc
     3541 ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag
     3601 atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg
     3661 ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag
     3721 attctcatta aggccaagaa gggcggcaag atcgccgtga attctcacgg cttccctccc
     3781 gaggtggagg agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg
     3841 gatagacacc ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg cgactctaga
     3901 acgcgtcact gcggctnnnn nnnnnnnnnn nnngactaca gacatcagcg accggttagt
     3961 aatgatcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4021 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4081 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4141 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4201 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4261 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4321 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4381 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4441 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4501 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4561 tggcgatggt accggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4621 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4681 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4801 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     4861 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     4921 ggatctgatc agcacctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4981 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5041 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5101 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc
     5161 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5221 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5281 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5341 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     5401 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     5461 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5521 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5581 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5641 tggacgagct gtacaagtaa ggtaccttta agaccaatga cttacaaggc agctgtagat
     5701 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5761 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5821 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5881 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5941 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6001 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     6061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     6181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6301 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     6361 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     6541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6901 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6961 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     7021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     9181 taaagggaac aaaagctgga gctgcaagct t
//
