LOCUS       Exported                9213 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2005
                     /label=HNF4
                     /note="HNF4"
     primer_bind     2006..2025
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2049..2079
                     /label=minP
                     /note="minP"
     TATA_signal     2055..2058
                     /label=TATA
                     /note="TATA"
     gene            2112..3887
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3671..3685
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3919..3935
                     /estimated_length=17
     unsure          3919..3935
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3936..3952
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3968..4565
                     /label=WPRE
                     /note="WPRE"
     promoter        4576..4884
                     /label=SV40
                     /note="SV40"
     gene            4943..5662
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5733..5786
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5734..5967
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6039..6170
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7029..7127
                     /label=bla promoter
                     /note="bla promoter"
     gene            7128..7988
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8133..8806
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcggcaa aggtcatggc aaaggtcatg gcaaaggtca
     1981 tggcaaaggt catggcaaag gtcatgatcc gagaacagat ctggcctcgg cggccaagct
     2041 tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc ggtactgttg
     2101 gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
     2161 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc
     2221 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
     2281 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
     2341 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
     2401 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
     2461 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
     2521 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
     2581 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
     2641 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
     2701 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     2761 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     2821 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     2881 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     2941 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta
     3001 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     3061 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     3121 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     3181 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     3241 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     3301 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct
     3361 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     3421 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     3481 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     3541 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     3601 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     3661 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     3721 agattctcat taaggccaag aagggcggca agatcgccgt gaattctcac ggcttccctc
     3781 ccgaggtgga ggagcaggcc gccggcaccc tgcccatgag ctgcgcccag gagagcggca
     3841 tggatagaca ccctgctgct tgcgccagcg ccaggatcaa cgtctaaggc cgcgactcta
     3901 gaacgcgtca ctgcggctnn nnnnnnnnnn nnnnngacta cagacatcag cgaccggtta
     3961 gtaatgatcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4021 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4081 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4141 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4201 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4261 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4321 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4381 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4441 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4501 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4561 cctggcgatg gtaccggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4621 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4681 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4801 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4861 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4921 tcggatctga tcagcaccta gcatggtgag caagggcgag gagctgttca ccggggtggt
     4981 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5041 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5101 gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc
     5161 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5221 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5281 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5341 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
     5401 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
     5461 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5521 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5581 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5641 catggacgag ctgtacaagt aaggtacctt taagaccaat gacttacaag gcagctgtag
     5701 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5761 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5821 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5881 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5941 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6001 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6301 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6361 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6421 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6481 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6841 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6901 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6961 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7021 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8281 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8821 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8881 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8941 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9001 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9061 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9121 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     9181 actaaaggga acaaaagctg gagctgcaag ctt
//
