LOCUS       Exported                9237 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2029
                     /label=STAT1
                     /note="STAT1"
     primer_bind     2030..2049
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2073..2103
                     /label=minP
                     /note="minP"
     TATA_signal     2079..2082
                     /label=TATA
                     /note="TATA"
     gene            2136..3911
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3695..3709
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3943..3959
                     /estimated_length=17
     unsure          3943..3959
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3960..3976
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3992..4589
                     /label=WPRE
                     /note="WPRE"
     promoter        4600..4908
                     /label=SV40
                     /note="SV40"
     gene            4967..5686
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5757..5810
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5758..5991
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6063..6194
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7053..7151
                     /label=bla promoter
                     /note="bla promoter"
     gene            7152..8012
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8157..8830
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcagttt catattactc taaatcagtt tcatattact
     1981 ctaaatcagt ttcatattac tctaaatcag tttcatatta ctctaaatcg atccgagaac
     2041 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
     2101 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
     2161 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
     2221 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
     2281 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
     2341 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
     2401 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
     2461 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
     2521 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
     2581 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
     2641 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
     2701 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
     2761 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
     2821 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
     2881 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
     2941 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
     3001 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
     3061 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
     3121 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
     3181 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
     3241 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
     3301 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
     3361 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     3421 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
     3481 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
     3541 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
     3601 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
     3661 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
     3721 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
     3781 ccgtgaattc tcacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
     3841 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
     3901 tcaacgtcta aggccgcgac tctagaacgc gtcactgcgg ctnnnnnnnn nnnnnnnnng
     3961 actacagaca tcagcgaccg gttagtaatg atcgacaatc aacctctgga ttacaaaatt
     4021 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4081 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4141 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4201 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4261 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4321 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4381 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     4441 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4501 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4561 atctcccttt gggccgcctc cccgcctggc gatggtaccg gtgtggaaag tccccaggct
     4621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4681 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4741 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4801 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4861 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4921 tcccgggagc ttgtatatcc attttcggat ctgatcagca cctagcatgg tgagcaaggg
     4981 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5041 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     5101 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     5161 cacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     5221 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     5281 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     5341 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     5401 ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa
     5461 cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     5521 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     5581 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     5641 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggta cctttaagac
     5701 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5761 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     5821 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5881 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5941 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6001 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6061 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6181 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6241 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6301 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6361 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6421 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6481 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6541 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6601 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6781 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6841 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6901 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6961 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7021 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7381 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7681 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8941 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9001 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9061 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9121 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9181 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
