LOCUS       Exported                9225 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2017
                     /label=TFEB
                     /note="TFEB"
     primer_bind     2018..2037
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2061..2091
                     /label=minP
                     /note="minP"
     TATA_signal     2067..2070
                     /label=TATA
                     /note="TATA"
     gene            2124..3899
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3683..3697
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3931..3947
                     /estimated_length=17
     unsure          3931..3947
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3948..3964
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3980..4577
                     /label=WPRE
                     /note="WPRE"
     promoter        4588..4896
                     /label=SV40
                     /note="SV40"
     gene            4955..5674
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5745..5798
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5746..5979
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6051..6182
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7041..7139
                     /label=bla promoter
                     /note="bla promoter"
     gene            7140..8000
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8145..8818
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgtagg ccacgtgacc ggggtaggcc acgtgaccgg
     1981 ggtaggccac gtgaccgggg taggccacgt gaccggggat ccgagaacag atctggcctc
     2041 ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca gcttggcaat
     2101 ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag ggcccagcgc
     2161 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
     2221 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
     2281 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
     2341 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
     2401 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
     2461 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
     2521 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
     2581 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
     2641 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
     2701 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
     2761 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
     2821 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
     2881 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
     2941 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
     3001 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
     3061 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
     3121 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
     3181 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
     3241 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
     3301 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
     3361 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
     3421 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
     3481 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
     3541 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
     3601 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
     3661 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
     3721 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgaattctc
     3781 acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg agctgcgccc
     3841 aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc aacgtctaag
     3901 gccgcgactc tagaacgcgt cactgcggct nnnnnnnnnn nnnnnnngac tacagacatc
     3961 agcgaccggt tagtaatgat cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     4381 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4561 gccgcctccc cgcctggcga tggtaccggt gtggaaagtc cccaggctcc ccagcaggca
     4621 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4681 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4741 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4861 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4921 gtatatccat tttcggatct gatcagcacc tagcatggtg agcaagggcg aggagctgtt
     4981 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     5041 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     5101 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt
     5161 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5221 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5281 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5341 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     5401 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
     5461 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5521 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5581 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5641 gatcactctc ggcatggacg agctgtacaa gtaaggtacc tttaagacca atgacttaca
     5701 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5761 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     5821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6001 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6061 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6121 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6181 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6301 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     6361 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     6421 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6481 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6541 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6601 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6661 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6721 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6781 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6841 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6901 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6961 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     7021 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7081 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7141 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7201 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7261 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7321 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7381 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7441 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7501 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7561 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7621 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7681 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7741 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7801 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7861 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7921 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7981 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8941 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9001 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9061 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9121 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     9181 caattaaccc tcactaaagg gaacaaaagc tggagctgca agctt
//
