LOCUS       Exported                9209 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2001
                     /label=BMAL1(ARNTL)/CLOCK
                     /note="BMAL1(ARNTL)/CLOCK"
     primer_bind     2002..2021
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2045..2075
                     /label=minP
                     /note="minP"
     TATA_signal     2051..2054
                     /label=TATA
                     /note="TATA"
     gene            2108..3883
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3667..3681
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3915..3931
                     /estimated_length=17
     unsure          3915..3931
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3932..3948
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3964..4561
                     /label=WPRE
                     /note="WPRE"
     promoter        4572..4880
                     /label=SV40
                     /note="SV40"
     gene            4939..5658
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5729..5782
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5730..5963
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6035..6166
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7025..7123
                     /label=bla promoter
                     /note="bla promoter"
     gene            7124..7984
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8129..8802
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatccggtc acgttttccc ggtcacgttt tcccggtcac
     1981 gttttcccgg tcacgttttc cgatccgaga acagatctgg cctcggcggc caagcttaga
     2041 cactagaggg tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa
     2101 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
     2161 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
     2221 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
     2281 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
     2341 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
     2401 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     2461 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     2521 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     2581 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     2641 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     2701 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     2761 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     2821 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     2881 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     2941 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     3001 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     3061 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     3121 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     3181 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     3241 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     3301 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     3361 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     3421 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     3481 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     3541 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     3601 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     3661 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     3721 tctcattaag gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga
     3781 ggtggaggag caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga
     3841 tagacaccct gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagaac
     3901 gcgtcactgc ggctnnnnnn nnnnnnnnnn ngactacaga catcagcgac cggttagtaa
     3961 tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4021 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4081 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4141 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4201 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4261 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4321 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4381 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4441 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4501 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4561 gcgatggtac cggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4621 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4681 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4801 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     4861 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     4921 atctgatcag cacctagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4981 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     5041 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     5101 cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcgcccgc
     5161 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     5221 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     5281 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     5341 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     5401 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     5461 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5521 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5581 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5641 gacgagctgt acaagtaagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5701 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5761 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5821 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5881 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5941 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6001 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6121 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6301 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     6361 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6421 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6481 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6961 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7021 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7081 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7141 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7201 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7261 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7321 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7381 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8281 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8881 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8941 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9001 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9061 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9121 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     9181 aagggaacaa aagctggagc tgcaagctt
//
