LOCUS       Exported                9221 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2013
                     /label=SP1
                     /note="SP1"
     primer_bind     2014..2033
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2057..2087
                     /label=minP
                     /note="minP"
     TATA_signal     2063..2066
                     /label=TATA
                     /note="TATA"
     gene            2120..3895
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3679..3693
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3927..3943
                     /estimated_length=17
     unsure          3927..3943
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3944..3960
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3976..4573
                     /label=WPRE
                     /note="WPRE"
     promoter        4584..4892
                     /label=SV40
                     /note="SV40"
     gene            4951..5670
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5741..5794
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5742..5975
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6047..6178
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7037..7135
                     /label=bla promoter
                     /note="bla promoter"
     gene            7136..7996
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8141..8814
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcattcg atcggggcgg ggcgagatta gattcgatcg
     1981 gggcggggcg agattcgatc ggggcggggc gaggatccga gaacagatct ggcctcggcg
     2041 gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg
     2101 tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt
     2161 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
     2221 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
     2281 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
     2341 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
     2401 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
     2461 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
     2521 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
     2581 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
     2641 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
     2701 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
     2761 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
     2821 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
     2881 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
     2941 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
     3001 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
     3061 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
     3121 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
     3181 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
     3241 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
     3301 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
     3361 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
     3421 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
     3481 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
     3541 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
     3601 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
     3661 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
     3721 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtga attctcacgg
     3781 cttccctccc gaggtggagg agcaggccgc cggcaccctg cccatgagct gcgcccagga
     3841 gagcggcatg gatagacacc ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg
     3901 cgactctaga acgcgtcact gcggctnnnn nnnnnnnnnn nnngactaca gacatcagcg
     3961 accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4021 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4081 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4141 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4201 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4261 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4321 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4381 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4441 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4501 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4561 cctccccgcc tggcgatggt accggtgtgg aaagtcccca ggctccccag caggcagaag
     4621 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4681 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4741 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4801 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4861 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4921 atccattttc ggatctgatc agcacctagc atggtgagca agggcgagga gctgttcacc
     4981 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5041 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5101 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag
     5161 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5221 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5281 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5341 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     5401 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     5461 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5521 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     5581 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5641 actctcggca tggacgagct gtacaagtaa ggtaccttta agaccaatga cttacaaggc
     5701 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5761 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5821 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5881 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5941 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6001 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6061 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6121 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6181 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6241 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6361 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6421 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6481 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6541 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6841 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6901 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6961 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     7021 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7081 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7141 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7201 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7261 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7321 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7381 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7441 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7501 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7561 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7621 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7681 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7741 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7801 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7861 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7921 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7981 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8041 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8281 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8761 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8821 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8881 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8941 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9001 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9061 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9121 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9181 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
