LOCUS       Exported                9215 bp DNA     circular SYN 24-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2007
                     /label=AP1 (c-Fos, c-Jun, ATF and JDP families)
                     /note="AP1 (c-Fos, c-Jun, ATF and JDP families)"
     primer_bind     2008..2027
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2051..2081
                     /label=minP
                     /note="minP"
     TATA_signal     2057..2060
                     /label=TATA
                     /note="TATA"
     gene            2114..3889
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3673..3687
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3921..3937
                     /estimated_length=17
     unsure          3921..3937
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3938..3954
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3970..4567
                     /label=WPRE
                     /note="WPRE"
     promoter        4578..4886
                     /label=SV40
                     /note="SV40"
     gene            4945..5664
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5735..5788
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5736..5969
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6041..6172
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7031..7129
                     /label=bla promoter
                     /note="bla promoter"
     gene            7130..7990
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8135..8808
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctgagt cagtgagtca ctgactcact gactcatgag
     1981 tcagctgact ctgagtcagt gagtcaggat ccgagaacag atctggcctc ggcggccaag
     2041 cttagacact agagggtata taatggaagc tcgacttcca gcttggcaat ccggtactgt
     2101 tggtaaagcc accatggaag atgccaaaaa cattaagaag ggcccagcgc cattctaccc
     2161 actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt
     2221 gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta
     2281 cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca
     2341 tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct
     2401 gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa
     2461 cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat
     2521 cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac
     2581 cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt
     2641 caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat
     2701 gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg
     2761 tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc
     2821 tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt
     2881 gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag
     2941 cttgcaagac tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc
     3001 taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg
     3061 ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat
     3121 ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga
     3181 cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt
     3241 ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat
     3301 gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg
     3361 ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga
     3421 ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag
     3481 catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga
     3541 tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa
     3601 ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt
     3661 tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg
     3721 cgagattctc attaaggcca agaagggcgg caagatcgcc gtgaattctc acggcttccc
     3781 tcccgaggtg gaggagcagg ccgccggcac cctgcccatg agctgcgccc aggagagcgg
     3841 catggataga caccctgctg cttgcgccag cgccaggatc aacgtctaag gccgcgactc
     3901 tagaacgcgt cactgcggct nnnnnnnnnn nnnnnnngac tacagacatc agcgaccggt
     3961 tagtaatgat cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4381 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4561 cgcctggcga tggtaccggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4801 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4861 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4921 tttcggatct gatcagcacc tagcatggtg agcaagggcg aggagctgtt caccggggtg
     4981 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5041 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     5101 aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc
     5161 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5221 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5281 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5341 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
     5401 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
     5461 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5521 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5581 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5641 ggcatggacg agctgtacaa gtaaggtacc tttaagacca atgacttaca aggcagctgt
     5701 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5761 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5821 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5881 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5941 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6001 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6181 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6301 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6361 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6541 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6601 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6661 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6721 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6781 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6841 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6901 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6961 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7261 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7321 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7381 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7441 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7501 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7561 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7621 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7681 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7741 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7801 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7861 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7921 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7981 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8041 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8821 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8941 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9001 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9061 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     9181 tcactaaagg gaacaaaagc tggagctgca agctt
//
