LOCUS       90387-attachment        9240 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2032
                     /label=IFNB1
                     /note="IFNB1"
     primer_bind     2033..2052
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2076..2106
                     /label=minP
                     /note="minP"
     TATA_signal     2082..2085
                     /label=TATA
                     /note="TATA"
     gene            2139..3914
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3698..3712
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3946..3962
                     /estimated_length=17
     unsure          3946..3962
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3963..3979
                     /label=cDNA &amp
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    3995..4592
                     /label=WPRE
                     /note="WPRE"
     promoter        4603..4911
                     /label=SV40
                     /note="SV40"
     gene            4970..5689
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5760..5813
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5761..5994
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6066..6197
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7056..7154
                     /label=bla promoter
                     /note="bla promoter"
     gene            7155..8015
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8160..8833
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctagaa actactaaaa tgtaaatgac ataggaaaac
     1981 tgaaactgag aacggaaact gggaaattcc cctgaataga gagaggacca tcgatccgag
     2041 aacagatctg gcctcggcgg ccaagcttag acactagagg gtatataatg gaagctcgac
     2101 ttccagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta
     2161 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
     2221 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
     2281 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
     2341 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
     2401 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
     2461 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     2521 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     2581 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     2641 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     2701 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     2761 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     2821 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     2881 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     2941 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     3001 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     3061 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     3121 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     3181 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     3241 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     3301 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     3361 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     3421 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     3481 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     3541 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     3601 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     3661 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     3721 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     3781 tcgccgtgaa ttctcacggc ttccctcccg aggtggagga gcaggccgcc ggcaccctgc
     3841 ccatgagctg cgcccaggag agcggcatgg atagacaccc tgctgcttgc gccagcgcca
     3901 ggatcaacgt ctaaggccgc gactctagaa cgcgtcactg cggctnnnnn nnnnnnnnnn
     3961 nngactacag acatcagcga ccggttagta atgatcgaca atcaacctct ggattacaaa
     4021 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4081 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4141 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4201 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4261 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4321 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4381 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4441 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4501 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4561 cggatctccc tttgggccgc ctccccgcct ggcgatggta ccggtgtgga aagtccccag
     4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4741 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4801 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4861 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4921 agctcccggg agcttgtata tccattttcg gatctgatca gcacctagca tggtgagcaa
     4981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5161 cttcacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     5221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5401 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     5461 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5581 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag gtacctttaa
     5701 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5761 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6001 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6061 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6181 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6361 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     6421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     6601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
