LOCUS       90388-attachment        9265 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
     LTR             230..410
                     /label=HIV-1 5' LTR
                     /note="HIV-1 5' LTR"
     misc_feature    520
                     /label=5' splice donor
                     /note="5' splice donor"
     misc_feature    521..565
                     /label=HIV-1 psi packaging signal
                     /note="HIV-1 psi packaging signal"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
                     /note="HIV-1 Rev response element (RRE)"
     misc_feature    1656
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1684
                     /label=3' splice acceptor
                     /note="3' splice acceptor"
     misc_feature    1801..1923
                     /label=cPPT
                     /note="cPPT"
     primer_bind     1929..1945
                     /label=pL_RE_IF_F
                     /note="pL_RE_IF_F"
     enhancer        1946..2057
                     /label=IRF3/7
                     /note="IRF3/7"
     primer_bind     2058..2077
                     /label=pL_RE_IF_R
                     /note="pL_RE_IF_R"
     promoter        2101..2131
                     /label=minP
                     /note="minP"
     TATA_signal     2107..2110
                     /label=TATA
                     /note="TATA"
     gene            2164..3939
                     /label=Luc2P
                     /note="Luc2P"
     primer_bind     3723..3737
                     /label=TAG_seq_P2_X
                     /note="TAG_seq_P2_X"
     gap             3971..3987
                     /estimated_length=17
     unsure          3971..3987
                     /label=UMI RE-TAG
                     /note="UMI RE-TAG"
     primer_bind     3988..4004
                     /label=cDNA &amp
                     /label=cDNA &amp; TAG_seq_P1
                     /note="cDNA & TAG_seq_P1"
     misc_feature    4020..4617
                     /label=WPRE
                     /note="WPRE"
     promoter        4628..4936
                     /label=SV40
                     /note="SV40"
     gene            4995..5714
                     /label=EmGFP
                     /note="EmGFP"
     misc_feature    5785..5838
                     /label=Delta U3
                     /note="Delta U3"
     LTR             5786..6019
                     /label=3' LTR
                     /note="3' LTR"
     polyA_signal    6091..6222
                     /label=SV40 Poly A signal
                     /note="SV40 Poly A signal"
     promoter        7081..7179
                     /label=bla promoter
                     /note="bla promoter"
     gene            7180..8040
                     /label=Amp R
                     /note="Amp R"
     rep_origin      8185..8858
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgagaa gtgaaagtgg agaagtgaaa gtggagaagt
     1981 gaaagtggag aagtgaaagt ggagaagtga aagtggagaa gtgaaagtgg agaagtgaaa
     2041 gtggagaagt gaaagtggat ccgagaacag atctggcctc ggcggccaag cttagacact
     2101 agagggtata taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc
     2161 accatggaag atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac
     2221 gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc
     2281 atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg
     2341 agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg
     2401 gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt
     2461 gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc
     2521 atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg
     2581 caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag
     2641 ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac
     2701 gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt
     2761 ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc
     2821 agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc
     2881 gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc
     2941 tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac
     3001 tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact
     3061 ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc
     3121 agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc
     3181 tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct
     3241 ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt
     3301 aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc
     3361 ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac
     3421 agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag
     3481 agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg
     3541 caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag
     3601 ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg
     3661 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
     3721 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
     3781 attaaggcca agaagggcgg caagatcgcc gtgaattctc acggcttccc tcccgaggtg
     3841 gaggagcagg ccgccggcac cctgcccatg agctgcgccc aggagagcgg catggataga
     3901 caccctgctg cttgcgccag cgccaggatc aacgtctaag gccgcgactc tagaacgcgt
     3961 cactgcggct nnnnnnnnnn nnnnnnngac tacagacatc agcgaccggt tagtaatgat
     4021 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4081 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4141 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4201 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4261 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4321 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4381 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4441 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4501 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4561 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggcga
     4621 tggtaccggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4681 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4741 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4801 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4861 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     4921 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     4981 gatcagcacc tagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5041 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5101 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     5161 tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc gcccgctacc
     5221 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5281 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5341 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5401 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     5461 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     5521 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5581 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     5641 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5701 agctgtacaa gtaaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5761 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5821 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5881 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5941 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6001 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6061 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     6241 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6301 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6361 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     6421 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6481 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6541 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     6601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6781 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6841 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6901 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6961 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7021 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     7081 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7141 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7201 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7261 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7321 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7381 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     7441 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7501 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7561 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7621 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7681 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7741 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7801 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7861 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7921 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7981 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8041 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8341 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9001 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9061 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9181 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     9241 gaacaaaagc tggagctgca agctt
//
