LOCUS       pKD233.7-3              5437 bp ds-DNA     circular     07-OCT-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(238..920)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(3751..3755)
                     /label="Repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3733..3817
                     /label="Psbal195_3860 (185-269)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2857..3477
                     /label="Sbal195_3858 (TtrR)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1898..2608
                     /label="mCherry (RBS 7.923)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3815..3815
                     /label="+1 (S. typ)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3019..3021
                     /label="D55"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3616..3720
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3740..3753
                     /label="Fnr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             1877..1890
                     /label="RBS pprotet"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    complement(3769..3773)
                     /label="Repeat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2822..2856
                     /label="sRBS (1087.31)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2748..2801
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1835..1869
                     /label="Constitutive promoter J23114"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4777..4810
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1879..1890
                     /label="B0034"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     CDS             4811..5437
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2773..2791
                     /label="TetR2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3802..3807
                     /label="-10 (S. typ)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3781..3786
                     /label="-10"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3755..3760
                     /label="-35"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3755..3759
                     /label="Repeat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3836..4555
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3747..3758
                     /label="Repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4742..4776
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3487..3615
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2748..2766
                     /label="TetR1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      925..1030
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     promoter        2802..2821
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    3793..3793
                     /label="+1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1047..1706
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3818..3835
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3802..3808
                     /label="Repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(3814..3820)
                     /label="Repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3735..3739
                     /label="Repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      1..105
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    4568..4696
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3779..3784
                     /label="-35 (S. typ)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2609..2668
                     /label="L3S1P52"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
       61 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCC CTAGACCTAG GCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
      181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
      241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
      481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      901 TTAAGGGATT TTGGTCATGA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
      961 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     1021 GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT
     1081 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA
     1141 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG
     1201 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG
     1261 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT
     1321 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG
     1381 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG
     1441 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA
     1501 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC
     1561 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA
     1621 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG
     1681 GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1741 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG
     1801 TGCCGATCAA CGTCTCATTT TCGCCAGATA TCGAtttatg gctagctcag tcctaggtac
     1861 aatgctagcg aattcattaa agaggagaaa ggtacccatg gtgagcaagg gcgaggagga
     1921 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1981 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2041 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2101 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2161 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2221 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2281 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2341 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     2401 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     2461 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     2521 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     2581 cggcggcatg gacgagctgt acaagtaaGC TTTCTAACTA AAAAGGCCTC CCAAATCGGG
     2641 GGGCCTTTTT TATTGATAAC AAAACGCTCG TCTAAGAAAC CATTATTATC ATGACATTAA
     2701 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA CCTCGAGTCC CTATCAGTGA
     2761 TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CATCAGCAGG ACGCACTGAC
     2821 CACTTTAATT CATTACCACA CTTAATAGGA GCGATAATGA GCCTGGCGCT GCCGGTGTAC
     2881 CTGATTGACG ACGATGACTC CGTTCGCCGT TCCCTGCGTT TCATGCTGGA AAGCTACGGC
     2941 CTGAAAATCA CTGACTTCGA TTCTGCTGAA GCGTTCTTCA CCGCGGTAGA CCTGACCCTG
     3001 CCGGGTTGCG CACTGGTGGA CGTACGTATG CCGGGCCTGA GCGGCCCACA GCTGCACCTG
     3061 GAACTGGTGT CTAAGAACAG CCCGCTGGCC GTGATCTATC TGACCGGTCA CGGCGACGTT
     3121 CCGATGGCGG TTGAAGCGCT GAAACTGGGT GCGGTAGATT TCTTTCAGAA ACCTGCAGAC
     3181 GGCGCTAAAC TGGCTGAAGC TGTGGTCAAA GCCCTGGAAC ACGCGAAAAC CCACTACCAA
     3241 GACAACCAGT ACCTGGAAAC CTATCAGGCT CTGACCCCAC GTGAGCGCGA AATCCTGAAC
     3301 CTGATTGCGC AAGGTCTGAA AAACCAGGAA ATCGCGGACA ACCTGTGCAT TGCGATGCGC
     3361 ACCGTAGAAA TCCACCGTGC GAACCTGATG AAAGGTATGC AGGTGGGCAG CCTGGCAGAG
     3421 ATGATGCTGA TTTACTCTCG TATCGCGGAG CGTCTGCGCC TGGAAGATAA CCGTTAATAA
     3481 TCTAGACCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA
     3541 TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC TGGCTCACCT TCGGGTGGGC
     3601 CTTTCTGCGT TTATAGTAAA TCACTGCATA ATTCGTGTCG CTCAAGGCGC ACTCCCGTTC
     3661 TGGATAATGT TTTTTGCGCC GACATCATAA CGGTTCTGGC AAATATTCTG AAATGAGCTG
     3721 GTTAGCTAGT CAATATTTGT TGCGCTAGAT CAAATCCACG CCTGATATGT GGAAAACCAC
     3781 TATAGTTATG CCGCCTCGCC TTTTACAGAA TGCCTGTAAA GAGGAGAAAT ACTAGATGCG
     3841 TAAAGGCGAA GAGCTGTTCA CTGGTGTCGT CCCTATTCTG GTGGAACTGG ATGGTGATGT
     3901 CAACGGTCAT AAGTTTTCCG TGCGTGGCGA GGGTGAAGGT GACGCAACTA ATGGTAAACT
     3961 GACGCTGAAG TTCATCTGTA CTACTGGTAA ACTGCCGGTA CCTTGGCCGA CTCTGGTAAC
     4021 GACGCTGACT TATGGTGTTC AGTGCTTTGC TCGTTATCCG GACCATATGA AGCAGCATGA
     4081 CTTCTTCAAG TCCGCCATGC CGGAAGGCTA TGTGCAGGAA CGCACGATTT CCTTTAAGGA
     4141 TGACGGCACG TACAAAACGC GTGCGGAAGT GAAATTTGAA GGCGATACCC TGGTAAACCG
     4201 CATTGAGCTG AAAGGCATTG ACTTTAAAGA AGACGGCAAT ATCCTGGGCC ATAAGCTGGA
     4261 ATACAATTTT AACAGCCACA ATGTTTACAT CACCGCCGAT AAACAAAAAA ATGGCATTAA
     4321 AGCGAATTTT AAAATTCGCC ACAACGTGGA GGATGGCAGC GTGCAGCTGG CTGATCACTA
     4381 CCAGCAAAAC ACTCCAATCG GTGATGGTCC TGTTCTGCTG CCAGACAATC ACTATCTGAG
     4441 CACGCAAAGC GTTCTGTCTA AAGATCCGAA CGAGAAACGC GATCATATGG TTCTGCTGGA
     4501 GTTCGTAACC GCAGCGGGCA TCACGCATGG TATGGATGAA CTGTACAAAT GATGATAATA
     4561 ATCTAGACCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT
     4621 ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG
     4681 CCTTTCTGCG TTTATAGGTA CCCGTGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT
     4741 TTTTACGGCT AGCTCAGTCC TAGGTATAGT GCTAGCCCAG CCAGAGAAAC ACTCTTTAAC
     4801 AGGGGGTAGT ATGATGTCTC GTTTAGATAA AAGTAAAGTG ATTAACAGCG CATTAGAGCT
     4861 GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA CTCGCCCAGA AGCTAGGTGT
     4921 AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG GCTTTGCTCG ACGCCTTAGC
     4981 CATTGAGATG TTAGATAGGC ACCATACTCA CTTTTGCCCT TTAGAAGGGG AAAGCTGGCA
     5041 AGATTTTTTA CGTAATAACG CTAAAAGTTT TAGATGTGCT TTACTAAGTC ATCGCGATGG
     5101 AGCAAAAGTA CATTTAGGTA CACGGCCTAC AGAAAAACAG TATGAAACTC TCGAAAATCA
     5161 ATTAGCCTTT TTATGCCAAC AAGGTTTTTC ACTAGAGAAT GCATTATATG CACTCAGCGC
     5221 AGTGGGGCAT TTTACTTTAG GTTGCGTATT GGAAGATCAA GAGCATCAAG TCGCTAAAGA
     5281 AGAAAGGGAA ACACCTACTA CTGATAGTAT GCCGCCATTA TTACGACAAG CTATCGAATT
     5341 ATTTGATCAC CAAGGTGCAG AGCCAGCCTT CTTATTCGGC CTTGAATTGA TCATATGCGG
     5401 ATTAGAAAAA CAACTTAAAT GTGAAAGTGG GTCTTAA
//