LOCUS pKD236-4b 4049 bp ds-DNA circular 28-SEP-2015 DEFINITION . FEATURES Location/Qualifiers misc_feature 2014..2048 /label="J23104" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1909..2013 /label="pEXT20 expression vector source" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 terminator complement(948..1053) /label="T0" /ApEinfo_revcolor=#ff0517 /ApEinfo_fwdcolor=#ff0517 misc_feature 3876..4004 /label="Terminator B0015" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 rep_origin complement(1168..1855) /label="p15A" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2922..2922 /label="Mutation" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter 1..148 /label="specR_promoter_from_pKD13" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 3195..3197 /label="Catalytic His" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 3363..3365 /label="-phosphatase activity" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 149..940 /label="SpecR" /ApEinfo_revcolor=#ff00ff /ApEinfo_fwdcolor=#ff00ff misc_feature 2049..2081 /label="sRBS (271.83)" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 2525..2526 /label="Deletion" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 2082..3866 /label="Shal_3128" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA 61 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGATCTGA 121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC 181 TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT 241 ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT 301 GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT 361 GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT 421 TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA 481 ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT 541 GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA 601 GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC 661 GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC 721 CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG 781 GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA 841 TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA 901 CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA GAGCTCGCTT GGACTCCTGT 961 TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT GTTCAGAACG CTCGGTTGCC 1021 GCCGGGCGTT TTTTATTGGT GAGAATCCAA GCACTAGTAA CAACTTATAT CGTATGGGGC 1081 TGACTTCAGG TGCTACATTT GAAGAGATAA ATTGCACTGA AATCTAGTAA TATTTTATCT 1141 GATTAATAAG ATGATCTTCT TGAGATCGTT TTGGTCTGCG CGTAATCTCT TGCTCTGAAA 1201 ACGAAAAAAC CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT CTGAGCTACC AACTCTTTGA 1261 ACCGAGGTAA CTGGCTTGGA GGAGCGCAGT CACCAAAACT TGTCCTTTCA GTTTAGCCTT 1321 AACCGGCGCA TGACTTCAAG ACTAACTCCT CTAAATCAAT TACCAGTGGC TGCTGCCAGT 1381 GGTGCTTTTG CATGTCTTTC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 1441 CGGTCGGACT GAACGGGGGG TTCGTGCATA CAGTCCAGCT TGGAGCGAAC TGCCTACCCG 1501 GAACTGAGTG TCAGGCGTGG AATGAGACAA ACGCGGCCAT AACAGCGGAA TGACACCGGT 1561 AAACCGAAAG GCAGGAACAG GAGAGCGCAC GAGGGAGCCG CCAGGGGGAA ACGCCTGGTA 1621 TCTTTATAGT CCTGTCGGGT TTCGCCACCA CTGATTTGAG CGTCAGATTT CGTGATGCTT 1681 GTCAGGGGGG CGGAGCCTAT GGAAAAACGG CTTTGCCGCG GCCCTCTCAC TTCCCTGTTA 1741 AGTATCTTCC TGGCATCTTC CAGGAAATCT CCGCCCCGTT CGTAAGCCAT TTCCGCTCGC 1801 CGCAGTCGAA CGACCGAGCG TAGCGAGTCA GTGAGCGAGG AAGCGGAATA TATCCCCTAG 1861 GCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGT AAATCACTGC 1921 ATAATTCGTG TCGCTCAAGG CGCACTCCCG TTCTGGATAA TGTTTTTTGC GCCGACATCA 1981 TAACGGTTCT GGCAAATATT CTGAAATGAG CTGttgacag ctagctcagt cctaggtatt 2041 gtgctagcCT AGTATCGATC TCCATAACTA TCCTATAGAT CATGTCCCGC CTGCTGCTGT 2101 GTATCTGTGT TCTGCTGTTC TCTTCTGTGG CGTGGTCTAA ACCGCAGCAG TTTTATGTGG 2161 GCGTACTGGC TAACTGGGGT CATCAGCAAG CCGTTGAACG TTGGACCCCG ATGATGGAGT 2221 ATCTGAACGA ACATGTGCCG GACGCGGAAT TTCACGTCTA CCCGGGCAAC TTCAAAGCAC 2281 TGAACCTGGC AATGGAACTG GGCCAGATTC AGTTCATTAT CACTAACCCG GGCCAATATC 2341 TGTACCTGAG CAATCAGTAC CCGCTGTCTT GGCTGGCGAC CATGCGTTCT AAGCGTCACG 2401 ATGGTACCAC TTCTGCGATC GGTTCCGCCA TTATTGTCCG CGCGGACAGC GACTACCGCA 2461 CCCTGTACGA CCTGAAAGGT AAAGTGGTGG CTGCGTCCGA CCCGCATGCT CTGGGTGGCT 2521 ACCAAGCGAC CGTCGGTCTG ATGCATTCCC TGGGCATGGA TCCGGACACC TTCTTCGGTG 2581 AAACCAAGTT TCTGGGCTTT CCACTGGATC CGCTGCTGTA CCAAGTTCGT GATGGCAACG 2641 TTGACGCGGC CATTACCCCA CTGTGCACTC TGGAGGACAT GGTTGCACGC GGCGTACTGA 2701 AATCTTCCGA TTTTCGTGTG CTGAACCCTA GCCGCCCGGA TGGTGTAGAA TGCCAGTGCT 2761 CTACCACCCT GTACCCGAAC TGGTCTTTCG CTGCGACTGA GTCTGTATCC ACCGAACTGT 2821 CTAAAGAAAT CACGCAGGCA CTGCTGGAAC TGCCATCCGA CAGCCCGGCA GCTATCAAAG 2881 CGCAACTGAC CGGCTGGACC AGCCCGATCT CCCAACTGGC GGTAATCAAA CTGTTCAAAG 2941 AGCTGCACGT AAAAACCCCG GACTCTAGCC GTTGGGAAGC CGTTAAGAAG TGGCTGGAAG 3001 AAAACCGTCA CTGGGGTATC CTGTCTGTTC TGGTGTTCAT CATTGCAACG CTGTATCACC 3061 TGTGGATTGA ATACCGCTTC CACCAAAAAA GCTCTTCTCT GATCGAATCT GAACGTCAGC 3121 TGAAACAGCA AGCTGTTGCC CTGGAACGTC TGCAATCTGC TAGCATCGTT GGTGAAATTG 3181 GTGCGGGTCT GGCCCACGAG ATTAATCAGC CGATCGCTGC AATTACCTCT TATTCTGAAG 3241 GTGGCATCAT GCGCCTGCAA GGTAAAGAAC AGGCGGATAC GGATAGCTGC ATCGAACTGC 3301 TGGAAAAAAT CCACAAACAG AGCACTCGCG CAGGCGAAGT GGTGCACCGC ATCCGTGGTC 3361 TGCTGAAACG TCGTGAAGCG GTGATGGTAG ATGTTAACAT CCTGACCCTG GTGGAAGAAT 3421 CCATCAGCCT GCTGCGTCTG GAGCTGGCAC GTCGCGAAAT CCAGATCAAC ACTCAGATCA 3481 AAGGTGAACC GTTCTTCATT ACTGCCGACC GCGTTGGCCT GCTGCAAGTT CTGATTAACC 3541 TGATCAAAAA CTCCCTGGAC GCGATCGCTG AATCTGATAA TGCCCGTTCT GGTAAAATCA 3601 ACATCGAACT GGACTTTAAA GAGTACCAGG TAAACGTCTC CATCATCGAT AACGGTCCGG 3661 GCCTGGCGAT GGATTCTGAC ACTCTGATGG CTACGTTTTA CACTACCAAA ATGGATGGCC 3721 TGGGCCTGGG TCTGGCAATC TGCCGCGAAG TTATCAGCAA CCACGACGGC CACTTCCTGC 3781 TGTCCAACCG TGACGACGGC GTTCTGGGCT GTGTGGCAAC CCTGAATCTG AAAAAACGCG 3841 GTTCTGAAGT GCCGATCGAA GTCTAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC 3901 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA 3961 GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATAGGTACC CGGGGATCCT 4021 CTAGAGTCGA CCTGCAGGCA TGCAAGCTT //