LOCUS       pKD236-4b               4049 bp ds-DNA     circular     28-SEP-2015
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2014..2048
                     /label="J23104"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1909..2013
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      complement(948..1053)
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3876..4004
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     rep_origin      complement(1168..1855)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2922..2922
                     /label="Mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1..148
                     /label="specR_promoter_from_pKD13"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3195..3197
                     /label="Catalytic His"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3363..3365
                     /label="-phosphatase activity"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    149..940
                     /label="SpecR"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    2049..2081
                     /label="sRBS (271.83)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2525..2526
                     /label="Deletion"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2082..3866
                     /label="Shal_3128"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA
       61 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGATCTGA
      121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC
      181 TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT
      241 ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT
      301 GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT
      361 GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT
      421 TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA
      481 ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT
      541 GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA
      601 GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC
      661 GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC
      721 CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG
      781 GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA
      841 TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA
      901 CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA GAGCTCGCTT GGACTCCTGT
      961 TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT GTTCAGAACG CTCGGTTGCC
     1021 GCCGGGCGTT TTTTATTGGT GAGAATCCAA GCACTAGTAA CAACTTATAT CGTATGGGGC
     1081 TGACTTCAGG TGCTACATTT GAAGAGATAA ATTGCACTGA AATCTAGTAA TATTTTATCT
     1141 GATTAATAAG ATGATCTTCT TGAGATCGTT TTGGTCTGCG CGTAATCTCT TGCTCTGAAA
     1201 ACGAAAAAAC CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT CTGAGCTACC AACTCTTTGA
     1261 ACCGAGGTAA CTGGCTTGGA GGAGCGCAGT CACCAAAACT TGTCCTTTCA GTTTAGCCTT
     1321 AACCGGCGCA TGACTTCAAG ACTAACTCCT CTAAATCAAT TACCAGTGGC TGCTGCCAGT
     1381 GGTGCTTTTG CATGTCTTTC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     1441 CGGTCGGACT GAACGGGGGG TTCGTGCATA CAGTCCAGCT TGGAGCGAAC TGCCTACCCG
     1501 GAACTGAGTG TCAGGCGTGG AATGAGACAA ACGCGGCCAT AACAGCGGAA TGACACCGGT
     1561 AAACCGAAAG GCAGGAACAG GAGAGCGCAC GAGGGAGCCG CCAGGGGGAA ACGCCTGGTA
     1621 TCTTTATAGT CCTGTCGGGT TTCGCCACCA CTGATTTGAG CGTCAGATTT CGTGATGCTT
     1681 GTCAGGGGGG CGGAGCCTAT GGAAAAACGG CTTTGCCGCG GCCCTCTCAC TTCCCTGTTA
     1741 AGTATCTTCC TGGCATCTTC CAGGAAATCT CCGCCCCGTT CGTAAGCCAT TTCCGCTCGC
     1801 CGCAGTCGAA CGACCGAGCG TAGCGAGTCA GTGAGCGAGG AAGCGGAATA TATCCCCTAG
     1861 GCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGT AAATCACTGC
     1921 ATAATTCGTG TCGCTCAAGG CGCACTCCCG TTCTGGATAA TGTTTTTTGC GCCGACATCA
     1981 TAACGGTTCT GGCAAATATT CTGAAATGAG CTGttgacag ctagctcagt cctaggtatt
     2041 gtgctagcCT AGTATCGATC TCCATAACTA TCCTATAGAT CATGTCCCGC CTGCTGCTGT
     2101 GTATCTGTGT TCTGCTGTTC TCTTCTGTGG CGTGGTCTAA ACCGCAGCAG TTTTATGTGG
     2161 GCGTACTGGC TAACTGGGGT CATCAGCAAG CCGTTGAACG TTGGACCCCG ATGATGGAGT
     2221 ATCTGAACGA ACATGTGCCG GACGCGGAAT TTCACGTCTA CCCGGGCAAC TTCAAAGCAC
     2281 TGAACCTGGC AATGGAACTG GGCCAGATTC AGTTCATTAT CACTAACCCG GGCCAATATC
     2341 TGTACCTGAG CAATCAGTAC CCGCTGTCTT GGCTGGCGAC CATGCGTTCT AAGCGTCACG
     2401 ATGGTACCAC TTCTGCGATC GGTTCCGCCA TTATTGTCCG CGCGGACAGC GACTACCGCA
     2461 CCCTGTACGA CCTGAAAGGT AAAGTGGTGG CTGCGTCCGA CCCGCATGCT CTGGGTGGCT
     2521 ACCAAGCGAC CGTCGGTCTG ATGCATTCCC TGGGCATGGA TCCGGACACC TTCTTCGGTG
     2581 AAACCAAGTT TCTGGGCTTT CCACTGGATC CGCTGCTGTA CCAAGTTCGT GATGGCAACG
     2641 TTGACGCGGC CATTACCCCA CTGTGCACTC TGGAGGACAT GGTTGCACGC GGCGTACTGA
     2701 AATCTTCCGA TTTTCGTGTG CTGAACCCTA GCCGCCCGGA TGGTGTAGAA TGCCAGTGCT
     2761 CTACCACCCT GTACCCGAAC TGGTCTTTCG CTGCGACTGA GTCTGTATCC ACCGAACTGT
     2821 CTAAAGAAAT CACGCAGGCA CTGCTGGAAC TGCCATCCGA CAGCCCGGCA GCTATCAAAG
     2881 CGCAACTGAC CGGCTGGACC AGCCCGATCT CCCAACTGGC GGTAATCAAA CTGTTCAAAG
     2941 AGCTGCACGT AAAAACCCCG GACTCTAGCC GTTGGGAAGC CGTTAAGAAG TGGCTGGAAG
     3001 AAAACCGTCA CTGGGGTATC CTGTCTGTTC TGGTGTTCAT CATTGCAACG CTGTATCACC
     3061 TGTGGATTGA ATACCGCTTC CACCAAAAAA GCTCTTCTCT GATCGAATCT GAACGTCAGC
     3121 TGAAACAGCA AGCTGTTGCC CTGGAACGTC TGCAATCTGC TAGCATCGTT GGTGAAATTG
     3181 GTGCGGGTCT GGCCCACGAG ATTAATCAGC CGATCGCTGC AATTACCTCT TATTCTGAAG
     3241 GTGGCATCAT GCGCCTGCAA GGTAAAGAAC AGGCGGATAC GGATAGCTGC ATCGAACTGC
     3301 TGGAAAAAAT CCACAAACAG AGCACTCGCG CAGGCGAAGT GGTGCACCGC ATCCGTGGTC
     3361 TGCTGAAACG TCGTGAAGCG GTGATGGTAG ATGTTAACAT CCTGACCCTG GTGGAAGAAT
     3421 CCATCAGCCT GCTGCGTCTG GAGCTGGCAC GTCGCGAAAT CCAGATCAAC ACTCAGATCA
     3481 AAGGTGAACC GTTCTTCATT ACTGCCGACC GCGTTGGCCT GCTGCAAGTT CTGATTAACC
     3541 TGATCAAAAA CTCCCTGGAC GCGATCGCTG AATCTGATAA TGCCCGTTCT GGTAAAATCA
     3601 ACATCGAACT GGACTTTAAA GAGTACCAGG TAAACGTCTC CATCATCGAT AACGGTCCGG
     3661 GCCTGGCGAT GGATTCTGAC ACTCTGATGG CTACGTTTTA CACTACCAAA ATGGATGGCC
     3721 TGGGCCTGGG TCTGGCAATC TGCCGCGAAG TTATCAGCAA CCACGACGGC CACTTCCTGC
     3781 TGTCCAACCG TGACGACGGC GTTCTGGGCT GTGTGGCAAC CCTGAATCTG AAAAAACGCG
     3841 GTTCTGAAGT GCCGATCGAA GTCTAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC
     3901 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA
     3961 GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATAGGTACC CGGGGATCCT
     4021 CTAGAGTCGA CCTGCAGGCA TGCAAGCTT
//