LOCUS       pKD237-3a-2             4807 bp ds-DNA     circular     14-MAR-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3313..3441
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2623..2657
                     /label="J23105"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3919..4638
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3442..3546
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3759..3776
                     /label="Repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3816..3821
                     /label="-35"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3590..3599
                     /label="Repeat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3836..3841
                     /label="-10"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1773..2483
                     /label="mCherry (RBS 7.923)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3848..3848
                     /label="+1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3665..3682
                     /label="Repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3190..3300
                     /label="new gBlock"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1710..1744
                     /label="J23100"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3559..3900
                     /label="Promoter (Shal_3127)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3901..3918
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             922..1581
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             1752..1765
                     /label="RBS pprotet"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    4651..4779
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3718..3739
                     /label="CRP?"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(113..795)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1754..1765
                     /label="B0034"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    2692..3303
                     /label="shal_3129"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2658..2691
                     /label="sRBS (965.94)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(3606..3615)
                     /label="Repeat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2860..2862
                     /label="Phospho-asp"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      800..905
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2484..2543
                     /label="L3S1P52"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
       61 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
      121 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
      181 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
      241 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
      301 CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
      361 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
      421 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
      481 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
      541 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA
      601 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
      661 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
      721 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
      781 GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA AAAACGCCCG GCGGCAACCG
      841 AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA CTGGATCTAT CAACAGGAGT
      901 CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA
      961 TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG GCATGATGAA CCTGAATCGC
     1021 CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC
     1081 GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT
     1141 GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC
     1201 GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC
     1261 ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC
     1321 ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGAAATTCCG GATGAGCATT
     1381 CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC
     1441 GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC
     1501 TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA
     1561 TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA
     1621 AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG
     1681 ATCAACGTCT CATTTTCGCC AGATATCGAt tgacggctag ctcagtccta ggtacagtgc
     1741 tagcgaattc attaaagagg agaaaggtac ccatggtgag caagggcgag gaggataaca
     1801 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1861 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1921 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1981 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     2041 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2101 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     2161 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2221 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2281 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2341 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2401 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2461 gcatggacga gctgtacaag taaGCTTTCT AACTAAAAAG GCCTCCCAAA TCGGGGGGCC
     2521 TTTTTTATTG ATAACAAAAC GCTCGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     2581 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCACCTCG AGtttacggc tagctcagtc
     2641 ctaggtacta tgctagcAGA AATATAAAGA ACGATCTATT TATCCGCGTA CATGCAGCAG
     2701 CAAATCAACG GCCCGGTCTA CCTGGTGGAT GATGATGAAG CCATTATCGA CTCCATCGAT
     2761 TTTTTGATGG AGGGCTACGG TTACAAACTG AACTCGTTTA ACTGCGGCGA TCGCTTTTTG
     2821 GCAGAAGTCG ATCTCACCCA GGCAGGATGT GTAATTCTGG ATGCGCGTAT GCCAGGCTTA
     2881 ACTGGTCCTC AGGTGCAACA GCTGCTGAGC GACGCGAAAA GCCCGCTTGC GGTCATCTTC
     2941 CTGACCGGCC ATGGCGATGT TCCGATGGCG GTTGATGCGT TCAAAAATGG CGCGTTCGAT
     3001 TTCTTTCAAA AACCTGTGCC GGGTAGCTTG CTCAGTCAGT CAATTGCCAA AGGCTTGACT
     3061 TATTCAATCG ATCAACATCT GAAACGTACT AACCAAGCGT TAATCGACAC GCTCTCGGAA
     3121 CGCGAAGCTC AAATTTTTCA ACTGGTGATT GCAGGCAACA CCAACAAACA GATGGCTAAC
     3181 GAGCTTTGCG TGGCTATTCG TACCATTGAG GTTCACCGTA GCAAACTGAT GACCAAACTG
     3241 GGTGTTAACA ACCTGGCTGA ACTGGTTAAA CTGGCGCCGC TGCTGGCACA TAAATCCGAA
     3301 TAATAATCTA GACCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC
     3361 GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCTACTAGAG TCACACTGGC TCACCTTCGG
     3421 GTGGGCCTTT CTGCGTTTAT AGTAAATCAC TGCATAATTC GTGTCGCTCA AGGCGCACTC
     3481 CCGTTCTGGA TAATGTTTTT TGCGCCGACA TCATAACGGT TCTGGCAAAT ATTCTGAAAT
     3541 GAGCTGGTTA GCTAGTCATT CAAGCATTAT TATGCTGTTT TTTGAAGTGA ATGTGCGGCC
     3601 ATCTAGCCGC ACATTTTGCA TCTAAAACAT GCAGTCATCA GCAAAATAAT AAACTTTTCC
     3661 CCAATATGTG GTTTACCACA ATTTACAGGA ATTCACTCCT GTGGTGGTGC AAATTTGAAC
     3721 TGTGAATTGC TTCACAAACG CCGCTATCGC AATGTCAGTA TGTGGTTTAC CACAATATCT
     3781 AATATCACTC TGCTCAATAA CAATGATGAA AACCTTAGGA AGAAGTTAAT TGTGTTAAAC
     3841 AGTTAACTAG GGGCTTTATC TAACGCTCTC CTAAGGACAA CTGTCATTGG GAGATTTAAC
     3901 AAAGAGGAGA AATACTAGAT GCGTAAAGGC GAAGAGCTGT TCACTGGTGT CGTCCCTATT
     3961 CTGGTGGAAC TGGATGGTGA TGTCAACGGT CATAAGTTTT CCGTGCGTGG CGAGGGTGAA
     4021 GGTGACGCAA CTAATGGTAA ACTGACGCTG AAGTTCATCT GTACTACTGG TAAACTGCCG
     4081 GTACCTTGGC CGACTCTGGT AACGACGCTG ACTTATGGTG TTCAGTGCTT TGCTCGTTAT
     4141 CCGGACCATA TGAAGCAGCA TGACTTCTTC AAGTCCGCCA TGCCGGAAGG CTATGTGCAG
     4201 GAACGCACGA TTTCCTTTAA GGATGACGGC ACGTACAAAA CGCGTGCGGA AGTGAAATTT
     4261 GAAGGCGATA CCCTGGTAAA CCGCATTGAG CTGAAAGGCA TTGACTTTAA AGAAGACGGC
     4321 AATATCCTGG GCCATAAGCT GGAATACAAT TTTAACAGCC ACAATGTTTA CATCACCGCC
     4381 GATAAACAAA AAAATGGCAT TAAAGCGAAT TTTAAAATTC GCCACAACGT GGAGGATGGC
     4441 AGCGTGCAGC TGGCTGATCA CTACCAGCAA AACACTCCAA TCGGTGATGG TCCTGTTCTG
     4501 CTGCCAGACA ATCACTATCT GAGCACGCAA AGCGTTCTGT CTAAAGATCC GAACGAGAAA
     4561 CGCGATCATA TGGTTCTGCT GGAGTTCGTA ACCGCAGCGG GCATCACGCA TGGTATGGAT
     4621 GAACTGTACA AATGATGATA ATAATCTAGA CCAGGCATCA AATAAAACGA AAGGCTCAGT
     4681 CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC TACTAGAGTC
     4741 ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAG GTACCCGTGG ATCCTCTAGA
     4801 GTCGACC
//