LOCUS       4010_to_4731_of_        5008 bp    DNA     circular SYN 07-Apr-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     source          <618..629
                     /db_xref="taxon:47945"
                     /mol_type="genomic DNA"
                     /note=" [Split]"
                     /organism="Cloning vector pEGFP-N1"
     source          630..>1339
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     source          join(<1654..>5008,<1..>608)
                     /db_xref="taxon:47946"
                     /mol_type="genomic DNA"
                     /note="/dnas_title=""pEGFP-C1"" [Split]"
                     /organism="Cloning vector pEGFP-C1"
     primer_bind     41..60
                     /label=CJO-0030 (=CMV 1)
     primer_bind     complement(170..188)
                     /label=CJO-0031 (=CMV2)
     primer_bind     536..556
                     /label=CMV promoter sequencing primer binding site
     gene            593..1545
                     /note="CMVfor Sequencing Read 2015.09.15"
     primer_bind     <618..628
                     /label=CJO-0039 Binding Site
                     /note=" [Split]"
     CDS             630..1340
                     /note="mEmerald"
     gene            complement(732..1698)
                     /note="CJO-0045 Sequencing Read 2015.09.15"
     CDS             1341..1361
                     /note="Linker Peptide"
     CDS             1361..1652
                     /label=Sec61b
     gene            <1654..1704
                     /gene="egfp"
                     /note=" [Split]"
     primer_bind     complement(1719..1748)
                     /label=CJO-0045 Binding Site
     polyA_site      1824..1894
                     /label=SV40 polyadenylation signal
     CDS             1824..1894
                     /label=SV40 polyadenylation signal
     primer_bind     complement(1850..1869)
                     /note="SV40 polyA primer binding"
     rep_origin      complement(1941..2397)
                     /label=f1 Origin of Replication
     primer_bind     complement(2048..2068)
                     /label=CJO-0036 Primer Binding Site
     primer_bind     2554..2573
                     /label=CJO-0032 (=SV40 1)
     oriT            2737..2872
                     /label=SV40 Origin of Replication
     primer_bind     complement(2847..2867)
                     /label=CJO-0033 (=SV40 2)
     CDS             2921..3715
                     /codon_start=1
                     /db_xref="GI:1377916"
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02577.1"
                     /transl_table=11
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3894..3994
                     /label=HSV TK PolyA
     rep_origin      4297..4969
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=cyan; /ApEinfo_revcolor=cyan;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {0 1 2 0
                     0 -1} 0} width 5 offset 0"
     primer_bind     complement(4497..4517)
                     /label=CJO-0028 binding site
BASE COUNT      1197 A      1358 C      1329 G      1124 T         0 OTHER
ORIGIN      ?
        1 GTATTACCGC CATGCATTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
       61 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
      121 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
      181 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
      241 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
      301 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
      361 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
      421 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
      481 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
      541 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT
      601 CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
      661 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
      721 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
      781 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTGACCTAC GGCGTGCAGT
      841 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
      901 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
      961 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
     1021 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAAGG
     1081 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ACCCGCCACA
     1141 ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
     1201 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCAAG CTGAGCAAAG
     1261 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
     1321 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGCCTGGT CCGACCCCCA
     1381 GTGGCACTAA CGTGGGATCC TCAGGGCGCT CTCCCAGCAA AGCAGTGGCC GCCCGGGCGG
     1441 CGGGATCCAC TGTCCGGCAG AGGAAAAATG CCAGCTGTGG GACAAGGAGT GCAGGCCGCA
     1501 CAACCTCGGC AGGCACCGGG GGGATGTGGC GATTCTACAC AGAAGATTCA CCTGGGCTCA
     1561 AAGTTGGCCC TGTTCCAGTA TTGGTTATGA GTCTTCTGTT CATCGCTTCT GTATTTATGT
     1621 TGCACATTTG GGGCAAGTAC ACTCGTTCGT AGGAATTCTG CAGTCGACGG TACCGCGGGC
     1681 CCGGGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
     1741 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA
     1801 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
     1861 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
     1921 ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA
     1981 TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA
     2041 ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT
     2101 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC
     2161 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA
     2221 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC
     2281 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT
     2341 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCAGG
     2401 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC
     2461 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
     2521 GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG
     2581 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
     2641 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
     2701 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
     2761 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
     2821 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
     2881 TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
     2941 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
     3001 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
     3061 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT
     3121 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
     3181 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
     3241 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
     3301 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
     3361 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
     3421 AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
     3481 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
     3541 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
     3601 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
     3661 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
     3721 ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT
     3781 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG
     3841 ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCTAG GGGGAGGCTA
     3901 ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA
     3961 CAGAATAAAA CGCACGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC
     4021 TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC
     4081 TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG
     4141 GCGGCAGGCC CTGCCATAGC CTCAGGTTAC TCATATATAC TTTAGATTGA TTTAAAACTT
     4201 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC
     4261 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     4321 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     4381 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     4441 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     4501 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     4561 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     4621 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     4681 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
     4741 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     4801 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     4861 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     4921 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT
     4981 GCGTTATCCC CTGATTCTGT GGATAACC
//
