LOCUS 601_to_1313_of_m 4999 bp DNA circular SYN 07-Apr-2017 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers source <618..629 /db_xref="taxon:47945" /mol_type="genomic DNA" /note=" [Split]" /organism="Cloning vector pEGFP-N1" source 630..>1330 /mol_type="other DNA" /note=" [Split]" /organism="synthetic DNA construct" source join(<1645..>4999,<1..>608) /db_xref="taxon:47946" /mol_type="genomic DNA" /note="/dnas_title=""pEGFP-C1"" [Split]" /organism="Cloning vector pEGFP-C1" primer_bind 41..60 /label=CJO-0030 (=CMV 1) primer_bind complement(170..188) /label=CJO-0031 (=CMV2) primer_bind 536..556 /label=CMV promoter sequencing primer binding site primer_bind <618..628 /label=CJO-0039 Binding Site /note=" [Split]" CDS 630..>1330 /label=mApple /note=" [Split]" primer_bind 1043..1062 /note="mCherry-F primer binding site" CDS 1332..1352 /note="Linker Peptide" CDS 1352..1643 /label=Sec61b primer_bind complement(1710..1739) /label=CJO-0045 Binding Site polyA_site 1815..1885 /label=SV40 polyadenylation signal CDS 1815..1885 /label=SV40 polyadenylation signal primer_bind complement(1841..1860) /note="SV40 polyA primer binding" rep_origin complement(1932..2388) /label=f1 Origin of Replication primer_bind complement(2039..2059) /label=CJO-0036 Primer Binding Site primer_bind 2545..2564 /label=CJO-0032 (=SV40 1) oriT 2728..2863 /label=SV40 Origin of Replication primer_bind complement(2838..2858) /label=CJO-0033 (=SV40 2) CDS 2912..3706 /codon_start=1 /db_xref="GI:1377916" /product="neomycin phosphotransferase" /protein_id="AAB02577.1" /transl_table=11 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" CDS 3885..3985 /label=HSV TK PolyA rep_origin 4288..4960 /label=pUC origin /note="/ApEinfo_fwdcolor=cyan; /ApEinfo_revcolor=cyan; /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {0 1 2 0 0 -1} 0} width 5 offset 0" primer_bind complement(4488..4508) /label=CJO-0028 binding site BASE COUNT 1189 A 1336 C 1341 G 1133 T 0 OTHER ORIGIN ? 1 GTATTACCGC CATGCATTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC 61 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC 121 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG 181 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 241 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC 301 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 361 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG 421 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT 481 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA 541 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT 601 CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA GGGCGAGGAG AATAACATGG 661 CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG 721 AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGCCTTTCAG ACCGCTAAGC 781 TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA 841 TGTACGGCTC CAAGGTCTAC ATTAAGCACC CAGCCGACAT CCCCGACTAC TTCAAGCTGT 901 CCTTCCCCGA GGGCTTCAGG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCATTATTC 961 ACGTTAACCA GGACTCCTCC CTGCAGGACG GCGTGTTCAT CTACAAGGTG AAGCTGCGCG 1021 GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT 1081 CCGAGGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGAG CGAGATCAAG AAGAGGCTGA 1141 AGCTGAAGGA CGGCGGCCAC TACGCCGCCG AGGTCAAGAC CACCTACAAG GCCAAGAAGC 1201 CCGTGCAGCT GCCCGGCGCC TACATCGTCG ACATCAAGTT GGACATCGTG TCCCACAACG 1261 AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA 1321 TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGCCTGG TCCGACCCCC AGTGGCACTA 1381 ACGTGGGATC CTCAGGGCGC TCTCCCAGCA AAGCAGTGGC CGCCCGGGCG GCGGGATCCA 1441 CTGTCCGGCA GAGGAAAAAT GCCAGCTGTG GGACAAGGAG TGCAGGCCGC ACAACCTCGG 1501 CAGGCACCGG GGGGATGTGG CGATTCTACA CAGAAGATTC ACCTGGGCTC AAAGTTGGCC 1561 CTGTTCCAGT ATTGGTTATG AGTCTTCTGT TCATCGCTTC TGTATTTATG TTGCACATTT 1621 GGGGCAAGTA CACTCGTTCG TAGGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC 1681 ACCGGATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT 1741 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT 1801 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 1861 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 1921 TCTTAACGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA 1981 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA 2041 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA 2101 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA 2161 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC 2221 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA 2281 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG 2341 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT 2401 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 2461 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTCC 2521 TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 2581 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 2641 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 2701 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 2761 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 2821 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT 2881 CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 2941 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 3001 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 3061 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT 3121 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 3181 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 3241 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 3301 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 3361 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 3421 GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 3481 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 3541 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 3601 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 3661 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 3721 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC 3781 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC 3841 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT AACTGAAACA 3901 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 3961 ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT 4021 GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA 4081 CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC 4141 CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA 4201 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 4261 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT 4321 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 4381 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 4441 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 4501 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 4561 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 4621 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 4681 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 4741 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 4801 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 4861 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 4921 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 4981 CCTGATTCTG TGGATAACC //