LOCUS       601_to_1313_of_m        4999 bp    DNA     circular SYN 07-Apr-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     source          <618..629
                     /db_xref="taxon:47945"
                     /mol_type="genomic DNA"
                     /note=" [Split]"
                     /organism="Cloning vector pEGFP-N1"
     source          630..>1330
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     source          join(<1645..>4999,<1..>608)
                     /db_xref="taxon:47946"
                     /mol_type="genomic DNA"
                     /note="/dnas_title=""pEGFP-C1"" [Split]"
                     /organism="Cloning vector pEGFP-C1"
     primer_bind     41..60
                     /label=CJO-0030 (=CMV 1)
     primer_bind     complement(170..188)
                     /label=CJO-0031 (=CMV2)
     primer_bind     536..556
                     /label=CMV promoter sequencing primer binding site
     primer_bind     <618..628
                     /label=CJO-0039 Binding Site
                     /note=" [Split]"
     CDS             630..>1330
                     /label=mApple
                     /note=" [Split]"
     primer_bind     1043..1062
                     /note="mCherry-F primer binding site"
     CDS             1332..1352
                     /note="Linker Peptide"
     CDS             1352..1643
                     /label=Sec61b
     primer_bind     complement(1710..1739)
                     /label=CJO-0045 Binding Site
     polyA_site      1815..1885
                     /label=SV40 polyadenylation signal
     CDS             1815..1885
                     /label=SV40 polyadenylation signal
     primer_bind     complement(1841..1860)
                     /note="SV40 polyA primer binding"
     rep_origin      complement(1932..2388)
                     /label=f1 Origin of Replication
     primer_bind     complement(2039..2059)
                     /label=CJO-0036 Primer Binding Site
     primer_bind     2545..2564
                     /label=CJO-0032 (=SV40 1)
     oriT            2728..2863
                     /label=SV40 Origin of Replication
     primer_bind     complement(2838..2858)
                     /label=CJO-0033 (=SV40 2)
     CDS             2912..3706
                     /codon_start=1
                     /db_xref="GI:1377916"
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02577.1"
                     /transl_table=11
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3885..3985
                     /label=HSV TK PolyA
     rep_origin      4288..4960
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=cyan; /ApEinfo_revcolor=cyan;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {0 1 2 0
                     0 -1} 0} width 5 offset 0"
     primer_bind     complement(4488..4508)
                     /label=CJO-0028 binding site
BASE COUNT      1189 A      1336 C      1341 G      1133 T         0 OTHER
ORIGIN      ?
        1 GTATTACCGC CATGCATTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
       61 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
      121 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
      181 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
      241 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
      301 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
      361 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
      421 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
      481 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
      541 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT
      601 CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA GGGCGAGGAG AATAACATGG
      661 CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG
      721 AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGCCTTTCAG ACCGCTAAGC
      781 TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA
      841 TGTACGGCTC CAAGGTCTAC ATTAAGCACC CAGCCGACAT CCCCGACTAC TTCAAGCTGT
      901 CCTTCCCCGA GGGCTTCAGG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCATTATTC
      961 ACGTTAACCA GGACTCCTCC CTGCAGGACG GCGTGTTCAT CTACAAGGTG AAGCTGCGCG
     1021 GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT
     1081 CCGAGGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGAG CGAGATCAAG AAGAGGCTGA
     1141 AGCTGAAGGA CGGCGGCCAC TACGCCGCCG AGGTCAAGAC CACCTACAAG GCCAAGAAGC
     1201 CCGTGCAGCT GCCCGGCGCC TACATCGTCG ACATCAAGTT GGACATCGTG TCCCACAACG
     1261 AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA
     1321 TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGCCTGG TCCGACCCCC AGTGGCACTA
     1381 ACGTGGGATC CTCAGGGCGC TCTCCCAGCA AAGCAGTGGC CGCCCGGGCG GCGGGATCCA
     1441 CTGTCCGGCA GAGGAAAAAT GCCAGCTGTG GGACAAGGAG TGCAGGCCGC ACAACCTCGG
     1501 CAGGCACCGG GGGGATGTGG CGATTCTACA CAGAAGATTC ACCTGGGCTC AAAGTTGGCC
     1561 CTGTTCCAGT ATTGGTTATG AGTCTTCTGT TCATCGCTTC TGTATTTATG TTGCACATTT
     1621 GGGGCAAGTA CACTCGTTCG TAGGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC
     1681 ACCGGATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT
     1741 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
     1801 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     1861 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     1921 TCTTAACGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA
     1981 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA
     2041 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA
     2101 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA
     2161 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC
     2221 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA
     2281 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG
     2341 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT
     2401 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
     2461 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTCC
     2521 TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC
     2581 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA
     2641 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
     2701 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
     2761 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC
     2821 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT
     2881 CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
     2941 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
     3001 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
     3061 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT
     3121 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
     3181 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
     3241 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
     3301 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
     3361 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
     3421 GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
     3481 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
     3541 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
     3601 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA
     3661 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG
     3721 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC
     3781 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
     3841 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT AACTGAAACA
     3901 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA
     3961 ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT
     4021 GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA
     4081 CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC
     4141 CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     4201 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     4261 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     4321 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     4381 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     4441 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     4501 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     4561 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     4621 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     4681 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     4741 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     4801 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     4861 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     4921 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     4981 CCTGATTCTG TGGATAACC
//
