LOCUS       3805_to_3760_of_        5210 bp ss-DNA     circular SYN 21-Mar-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    7..12
                     /note="BAR ThyS"
     protein_bind    85..105
                     /note="lacO"
     CDS             150..956
                     /note="E. coli WT ThyS"
     protein_bind    969..1013
                     /note="S-Tag"
     CDS             complement(1332..1991)
                     /note="CAT"
     rep_origin      2353..3265
                     /note="P15A ori"
     CDS             complement(3389..4471)
                     /note="lacI gene"
     protein_bind    4616..4636
                     /note="lacO;"
     misc_binding    4694..4711
                     /note="His-Tag"
     CDS             4724..5203
                     /note="DHFR G121V"
     misc_feature    5204..5208
                     /note="BAR DHFR"
BASE COUNT      1296 A      1332 C      1322 G      1260 T         0 OTHER
ORIGIN      ?
        1 GGCCGCATAA TGCTTAAGTC GAACAGAAAG TAATCGTATT GTACACGGCC GCATAATCGA
       61 AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCA TCTTAGTATA
      121 TTAGTTAAGT ATAAGAAGGA GATATACATA TGGCAGATCT CATGAAACAG TACCTGGAGC
      181 TGATGCAAAA AGTTCTGGAT GAGGGTACCC AGAAAAACGA CCGCACGGGT ACCGGAACGC
      241 TGAGCATTTT TGGCCATCAG ATGCGTTTTA ACCTGCAGGA CGGATTCCCG CTGGTTACGA
      301 CCAAACGCTG CCACCTGCGT AGCATTATTC ATGAGCTGCT GTGGTTTCTG CAAGGTGACA
      361 CTAACATCGC GTATCTGCAC GAAAACAATG TGACGATCTG GGATGAATGG GCCGATGAAA
      421 ACGGCGATCT GGGCCCAGTG TATGGTAAAC AGTGGCGCGC CTGGCCAACG CCAGATGGCC
      481 GCCACATCGA TCAGATCACG ACCGTGCTGA ATCAACTGAA AAACGACCCG GACAGCCGCC
      541 GCATTATTGT TTCCGCGTGG AATGTGGGTG AACTGGATAA AATGGCGCTG GCGCCGTGCC
      601 ATGCATTCTT CCAGTTTTAC GTTGCGGACG GTAAGCTGAG CTGTCAACTT TATCAGCGCA
      661 GCTGCGATGT TTTCCTCGGC CTGCCGTTCA ATATCGCCAG CTACGCGTTA CTGGTGCATA
      721 TGATGGCGCA GCAGTGCGAC CTGGAGGTGG GTGATTTTGT CTGGACCGGC GGTGATACCC
      781 ACCTGTACAG CAACCATATG GACCAAACGC ATCTGCAATT GAGCCGTGAG CCGCGTCCGC
      841 TGCCGAAACT GATTATTAAG CGCAAACCCG AGAGCATCTT TGACTATCGT TTCGAAGACT
      901 TCGAGATTGA AGGCTACGAT CCGCACCCGG GTATCAAAGC GCCGGTGGCT ATCTAACTCG
      961 AGTCTGGTAA AGAAACCGCT GCTGCGAAAT TTGAACGCCA GCACATGGAC TCGTCTACTA
     1021 GCGCAGCTTA ATTAACCTAG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
     1081 GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAACC TCAGGCATTT GAGAAGCACA
     1141 CGGTCACACT GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA
     1201 TTTAACGACC CTGCCCTGAA CCGACGACCG GGTCGAATTT GCTTTCGAAT TTCTGCCATT
     1261 CATCCGCTTA TTATCACTTA TTCAGGCGTA GCACCAGGCG TTTAAGGGCA CCAATAACTG
     1321 CCTTAAAAAA ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC
     1381 ATTCTGCCGA CATGGAAGCC ATCACAGACG GCATGATGAA CCTGAATCGC CAGCGGCATC
     1441 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATAGTGA AAACGGGGGC GAAGAAGTTG
     1501 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG
     1561 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC
     1621 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC
     1681 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT
     1741 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG
     1801 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA
     1861 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT
     1921 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT
     1981 TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC
     2041 GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAACGTCT
     2101 CATTTTCGCC AAAAGTTGGC CCAGGGCTTC CCGGTATCAA CAGGGACACC AGGATTTATT
     2161 TATTCTGCGA AGTGATCTTC CGTCACAGGT ATTTATTCGG CGCAAAGTGC GTCGGGTGAT
     2221 GCTGCCAACT TACTGATTTA GTGTATGATG GTGTTTTTGA GGTGCTCCAG TGGCTTCTGT
     2281 TTCTATCAGC TGTCCCTCCT GTTCAGCTAC TGACGGGGTG GTGCGTAACG GCAAAAGCAC
     2341 CGCCGGACAT CAGCGCTAGC GGAGTGTATA CTGGCTTACT ATGTTGGCAC TGATGAGGGT
     2401 GTCAGTGAAG TGCTTCATGT GGCAGGAGAA AAAAGGCTGC ACCGGTGCGT CAGCAGAATA
     2461 TGTGATACAG GATATATTCC GCTTCCTCGC TCACTGACTC GCTACGCTCG GTCGTTCGAC
     2521 TGCGGCGAGC GGAAATGGCT TACGAACGGG GCGGAGATTT CCTGGAAGAT GCCAGGAAGA
     2581 TACTTAACAG GGAAGTGAGA GGGCCGCGGC AAAGCCGTTT TTCCATAGGC TCCGCCCCCC
     2641 TGACAAGCAT CACGAAATCT GACGCTCAAA TCAGTGGTGG CGAAACCCGA CAGGACTATA
     2701 AAGATACCAG GCGTTTCCCC TGGCGGCTCC CTCGTGCGCT CTCCTGTTCC TGCCTTTCGG
     2761 TTTACCGGTG TCATTCCGCT GTTATGGCCG CGTTTGTCTC ATTCCACGCC TGACACTCAG
     2821 TTCCGGGTAG GCAGTTCGCT CCAAGCTGGA CTGTATGCAC GAACCCCCCG TTCAGTCCGA
     2881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGAAAGAC ATGCAAAAGC
     2941 ACCACTGGCA GCAGCCACTG GTAATTGATT TAGAGGAGTT AGTCTTGAAG TCATGCGCCG
     3001 GTTAAGGCTA AACTGAAAGG ACAAGTTTTG GTGACTGCGC TCCTCCAAGC CAGTTACCTC
     3061 GGTTCAAAGA GTTGGTAGCT CAGAGAACCT TCGAAAAACC GCCCTGCAAG GCGGTTTTTT
     3121 CGTTTTCAGA GCAAGAGATT ACGCGCAGAC CAAAACGATC TCAAGAAGAT CATCTTATTA
     3181 ATCAGATAAA ATATTTCTAG ATTTCAGTGC AATTTATCTC TTCAAATGTA GCACCTGAAG
     3241 TCAGCCCCAT ACGATATAAG TTGTAATTCT CATGTTAGTC ATGCCCCGCG CCCACCGGAA
     3301 GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT
     3361 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
     3421 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
     3481 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
     3541 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
     3601 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
     3661 CTACCGAGAT GTCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
     3721 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
     3781 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
     3841 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
     3901 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
     3961 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
     4021 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
     4081 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA
     4141 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC
     4201 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA
     4261 GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC
     4321 GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG
     4381 AAACGTGGCT GGCCTGGTTC ACCACGCGGG AAACGGTCTG ATAAGAGACA CCGGCATACT
     4441 CTGCGACATC GTATAACGTT ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG
     4501 GGCGCTATCA TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA
     4561 CGCTCTCCCT TATGCGACTC CTGCATTAGG AAATTAATAC GACTCACTAT AGGGGAATTG
     4621 TGAGCGGATA ACAATTCCCC TGTAGAAATA ATTTTGTTTA ACTTTAATAA GGAGATATAC
     4681 CATGGGCAGC AGCCATCACC ATCATCACCA CAGCCAGGAT CCGATGATCA GTCTGATTGC
     4741 GGCGTTAGCG GTAGATCGCG TTATCGGCAT GGAAAACGCC ATGCCGTGGA ACCTGCCTGC
     4801 CGATCTCGCC TGGTTTAAAC GCAACACCTT AAATAAACCC GTGATTATGG GCCGCCATAC
     4861 CTGGGAATCA ATCGGTCGTC CGTTGCCAGG ACGCAAAAAT ATTATCCTCA GCAGTCAACC
     4921 GGGTACGGAC GATCGCGTAA CGTGGGTGAA GTCGGTGGAT GAAGCCATCG CGGCGTGTGG
     4981 TGACGTACCA GAAATCATGG TGATTGGCGG CGGTCGCGTT TATGAACAGT TCTTGCCAAA
     5041 AGCGCAAAAA CTGTATCTGA CGCATATCGA CGCAGAAGTG GAAGTCGACA CCCATTTCCC
     5101 GGATTACGAG CCGGATGACT GGGAATCGGT ATTCAGCGAA TTCCACGATG CTGATGCGCA
     5161 GAACTCTCAC AGCTATTGCT TTGAGATTCT GGAGCGGCGG TAACATAAGC
//
