LOCUS       971_to_13_of_pAC        5223 bp ss-DNA     circular SYN 21-Mar-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     protein_bind    12..56
                     /note="S-Tag"
     CDS             complement(375..1034)
                     /note="CAT"
     rep_origin      1396..2308
                     /note="P15A ori"
     CDS             complement(2432..3514)
                     /note="lacI gene"
     protein_bind    3659..3679
                     /note="lacO;"
     misc_binding    3737..3754
                     /note="His-Tag"
     CDS             3767..4246
                     /note="WT E. coli DHFR"
     misc_feature    4247..4251
                     /note="BAR DHFR"
     misc_feature    4272..>4277
                     /note="BAR ThyS"
     primer_bind     complement(4306..4325)
                     /note="DuetDOWN1 Primer"
     primer_bind     4306..4325
                     /note="DuetUP2 Primer"
     protein_bind    4352..4372
                     /note="lacO"
     CDS             4428..5222
                     /note="TYMS R166Q"
BASE COUNT      1298 A      1335 C      1330 G      1260 T         0 OTHER
ORIGIN      ?
        1 TCGAGTCTGG TAAAGAAACC GCTGCTGCGA AATTTGAACG CCAGCACATG GACTCGTCTA
       61 CTAGCGCAGC TTAATTAACC TAGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC
      121 TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA ACCTCAGGCA TTTGAGAAGC
      181 ACACGGTCAC ACTGCTTCCG GTAGTCAATA AACCGGTAAA CCAGCAATAG ACATAAGCGG
      241 CTATTTAACG ACCCTGCCCT GAACCGACGA CCGGGTCGAA TTTGCTTTCG AATTTCTGCC
      301 ATTCATCCGC TTATTATCAC TTATTCAGGC GTAGCACCAG GCGTTTAAGG GCACCAATAA
      361 CTGCCTTAAA AAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA
      421 AGCATTCTGC CGACATGGAA GCCATCACAG ACGGCATGAT GAACCTGAAT CGCCAGCGGC
      481 ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATAG TGAAAACGGG GGCGAAGAAG
      541 TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG
      601 ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC
      661 GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG
      721 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC
      781 CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAACT CCGGATGAGC ATTCATCAGG
      841 CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT
      901 AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA
      961 AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG
     1021 ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG
     1081 CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG
     1141 TCTCATTTTC GCCAAAAGTT GGCCCAGGGC TTCCCGGTAT CAACAGGGAC ACCAGGATTT
     1201 ATTTATTCTG CGAAGTGATC TTCCGTCACA GGTATTTATT CGGCGCAAAG TGCGTCGGGT
     1261 GATGCTGCCA ACTTACTGAT TTAGTGTATG ATGGTGTTTT TGAGGTGCTC CAGTGGCTTC
     1321 TGTTTCTATC AGCTGTCCCT CCTGTTCAGC TACTGACGGG GTGGTGCGTA ACGGCAAAAG
     1381 CACCGCCGGA CATCAGCGCT AGCGGAGTGT ATACTGGCTT ACTATGTTGG CACTGATGAG
     1441 GGTGTCAGTG AAGTGCTTCA TGTGGCAGGA GAAAAAAGGC TGCACCGGTG CGTCAGCAGA
     1501 ATATGTGATA CAGGATATAT TCCGCTTCCT CGCTCACTGA CTCGCTACGC TCGGTCGTTC
     1561 GACTGCGGCG AGCGGAAATG GCTTACGAAC GGGGCGGAGA TTTCCTGGAA GATGCCAGGA
     1621 AGATACTTAA CAGGGAAGTG AGAGGGCCGC GGCAAAGCCG TTTTTCCATA GGCTCCGCCC
     1681 CCCTGACAAG CATCACGAAA TCTGACGCTC AAATCAGTGG TGGCGAAACC CGACAGGACT
     1741 ATAAAGATAC CAGGCGTTTC CCCTGGCGGC TCCCTCGTGC GCTCTCCTGT TCCTGCCTTT
     1801 CGGTTTACCG GTGTCATTCC GCTGTTATGG CCGCGTTTGT CTCATTCCAC GCCTGACACT
     1861 CAGTTCCGGG TAGGCAGTTC GCTCCAAGCT GGACTGTATG CACGAACCCC CCGTTCAGTC
     1921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGAAA GACATGCAAA
     1981 AGCACCACTG GCAGCAGCCA CTGGTAATTG ATTTAGAGGA GTTAGTCTTG AAGTCATGCG
     2041 CCGGTTAAGG CTAAACTGAA AGGACAAGTT TTGGTGACTG CGCTCCTCCA AGCCAGTTAC
     2101 CTCGGTTCAA AGAGTTGGTA GCTCAGAGAA CCTTCGAAAA ACCGCCCTGC AAGGCGGTTT
     2161 TTTCGTTTTC AGAGCAAGAG ATTACGCGCA GACCAAAACG ATCTCAAGAA GATCATCTTA
     2221 TTAATCAGAT AAAATATTTC TAGATTTCAG TGCAATTTAT CTCTTCAAAT GTAGCACCTG
     2281 AAGTCAGCCC CATACGATAT AAGTTGTAAT TCTCATGTTA GTCATGCCCC GCGCCCACCG
     2341 GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGAGATCCCG GTGCCTAATG
     2401 AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
     2461 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
     2521 GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG
     2581 CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC AGGCGAAAAT
     2641 CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA TCGTCGTATC
     2701 CCACTACCGA GATGTCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG CGCATTGCGC
     2761 CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC TCATTCAGCA
     2821 TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT TCCGCTATCG
     2881 GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA CGCGCCGAGA
     2941 CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG ACCAGATGCT
     3001 CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG GGTGTCTGGT
     3061 CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT
     3121 CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG AGAAGATTGT
     3181 GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC ACCACGCTGG
     3241 CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC GCGTGCAGGG
     3301 CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT GCCCGCCAGT TGTTGTGCCA
     3361 CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC CACTTTTTCC CGCGTTTTCG
     3421 CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT CTGATAAGAG ACACCGGCAT
     3481 ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC CACCCTGAAT TGACTCTCTT
     3541 CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA TTCGATGGTG TCCGGGATCT
     3601 CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAATTAA TACGACTCAC TATAGGGGAA
     3661 TTGTGAGCGG ATAACAATTC CCCTGTAGAA ATAATTTTGT TTAACTTTAA TAAGGAGATA
     3721 TACCATGGGC AGCAGCCATC ACCATCATCA CCACAGCCAG GATCCGATGA TCAGTCTGAT
     3781 TGCGGCGTTA GCGGTAGATC GCGTTATCGG CATGGAAAAC GCCATGCCGT GGAACCTGCC
     3841 TGCCGATCTC GCCTGGTTTA AACGCAACAC CTTAAATAAA CCCGTGATTA TGGGCCGCCA
     3901 TACCTGGGAA TCAATCGGTC GTCCGTTGCC AGGACGCAAA AATATTATCC TCAGCAGTCA
     3961 ACCGGGTACG GACGATCGCG TAACGTGGGT GAAGTCGGTG GATGAAGCCA TCGCGGCGTG
     4021 TGGTGACGTA CCAGAAATCA TGGTGATTGG CGGCGGTCGC GTTTATGAAC AGTTCTTGCC
     4081 AAAAGCGCAA AAACTGTATC TGACGCATAT CGACGCAGAA GTGGAAGGCG ACACCCATTT
     4141 CCCGGATTAC GAGCCGGATG ACTGGGAATC GGTATTCAGC GAATTCCACG ATGCTGATGC
     4201 GCAGAACTCT CACAGCTATT GCTTTGAGAT TCTGGAGCGG CGGTAACAGG CGTCGACAAG
     4261 CTTGCGGCCG CGACAGATGC TTAAGTCGAA CAGAAAGTAA TCGTATTGTA CACGGCCGCA
     4321 TAATCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCATCT
     4381 TAGTATATTA GTTAAGTATA AGAAGGAGAT ATACATATGG CAGATCTATG AAACAGTACC
     4441 TGGAGCTGAT GCAAAAAGTT CTGGATGAGG GTACCCAGAA AAACGACCGC ACGGGTACCG
     4501 GAACGCTGAG CATTTTTGGC CATCAGATGC GTTTTAACCT GCAGGACGGA TTCCCGCTGG
     4561 TTACGACCAA ACGCTGCCAC CTGCGTAGCA TTATTCATGA GCTGCTGTGG TTTCTGCAAG
     4621 GTGACACTAA CATCGCGTAT CTGCACGAAA ACAATGTGAC GATCTGGGAT GAATGGGCCG
     4681 ATGAAAACGG CGATCTGGGC CCAGTGTATG GTAAACAGTG GCGCGCCTGG CCAACGCCAG
     4741 ATGGCCGCCA CATCGATCAG ATCACGACCG TGCTGAATCA ACTGAAAAAC GACCCGGACA
     4801 GCCGCCGCAT TATTGTTTCC GCGTGGAATG TGGGTGAACT GGATAAAATG GCGCTGGCGC
     4861 CGTGCCATGC ATTCTTCCAG TTTTACGTTG CGGACGGTAA GCTGAGCTGT CAACTTTATC
     4921 AGCAGAGCTG CGATGTTTTC CTCGGCCTGC CGTTCAATAT CGCCAGCTAC GCGTTACTGG
     4981 TGCATATGAT GGCGCAGCAG TGCGACCTGG AGGTGGGTGA TTTTGTCTGG ACCGGCGGTG
     5041 ATACCCACCT GTACAGCAAC CATATGGACC AAACGCATCT GCAATTGAGC CGTGAGCCGC
     5101 GTCCGCTGCC GAAACTGATT ATTAAGCGCA AACCCGAGAG CATCTTTGAC TATCGTTTCG
     5161 AAGACTTCGA GATTGAAGGC TACGATCCGC ACCCGGGTAT CAAAGCGCCG GTGGCTATCT
     5221 AAC
//
