LOCUS       pAOJ15                  4030 bp    DNA     circular     11-APR-2017
FEATURES             Location/Qualifiers
     gene            complement(1310..1960)
                     /dnas_title="tetR"
                     /gene="tetR"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             complement(1310..1960)
                     /dnas_title="tetR"
                     /gene="tetR"
                     /note="tetracycline resistance regulation"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR"
                     /protein_id="ABO32180.1"
                     /db_xref="GI:130693910"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     promoter        1963..1997
                     /note="PtetA"
     promoter        complement(1981..2015)
                     /note="PtetR"
     gene            2052..2528
                     /note="tse2"
     terminator      complement(2613..2656)
                     /note="bla terminator"
     gene            complement(2709..3569)
                     /dnas_title="bla"
                     /gene="bla"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     CDS             complement(2709..3569)
                     /dnas_title="bla"
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAB81041.1"
                     /db_xref="GI:2522427"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_difference 2849..2854
                     /note="Was BsaI site in pWSK29"
     rep_origin      join(3717..3717,3717..4030,1..270)
                     /dnas_title="oriV; IncP"
                     /note="oriV RK2"
     misc_feature    409..465
                     /note="multiple cloning site"
     promoter        complement(523..557)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     gene            complement(273..482)
                     /dnas_title="lacZ-alpha"
                     /gene="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQL"
     CDS             complement(273..482)
                     /dnas_title="lacZ-alpha"
                     /gene="lacZ-alpha"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase alpha peptide"
                     /protein_id="ABO32178.1"
                     /db_xref="GI:130693908"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQL"
     oriT            1041..1150
                     /note="incP oriT"
     promoter        complement(3607..3639)
                     /note="Pbla"
     terminator      772..923
                     /note="Predicted Terminator Region from pSIM18"
     terminator      complement(1190..1236)
                     /note="tetR terminator"
     CDS             2052..2528
                     /note="tse2"
                     /translation="MSYDYEKTSLTLYRAVFKANYDGDVGRYLHPDKELAEAAEVAPL
                     LHPTFDSPNTPGVPARAPDIVAGRDGLYAPDTGGTSVFDRAGVLRRADGDFVIPDGTD
                     IPPDLKVKQDSYNKRLQATHYTIMPAKPMYREVLMGQLDNFVRNAIRRQWEKARGL"
     source          1..4030
                     /dnas_title="pAOJ15"
ORIGIN      
        1 tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag
       61 ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt
      121 ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc
      181 atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct
      241 gtcagtgagg gccaagtttt ccgcgaggta tccaactgtt gggaagggcg atcggtgcgg
      301 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc
      421 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc
      481 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      541 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      601 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      661 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctcg gtcttgcctt gctcgtcggt
      721 gatgtacttc accagctccg cgaagtcgct cttctTGAGT CAGACCCCGT AGAAAAGATC
      781 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
      841 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TAACCCGGCC
      901 TCAGCGCCGG GTTTTCTTTG CCTCACGATC GGCTCTTCGt tcgtctggaa ggcagtacac
      961 cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc
     1021 atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
     1081 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     1141 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa gggcatgaaa
     1201 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
     1261 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
     1321 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
     1381 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
     1441 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
     1501 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
     1561 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
     1621 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
     1681 ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt
     1741 cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg
     1801 cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca
     1861 accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca
     1921 gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga
     1981 taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcCACAGG
     2041 GAAGTCCACG CATGTCCTAC GACTACGAGA AAACCAGCCT CACCCTCTAC CGGGCGGTAT
     2101 TCAAGGCCAA CTACGACGGC GACGTCGGTC GCTACCTGCA TCCCGACAAG GAACTCGCCG
     2161 AGGCTGCGGA AGTCGCCCCG CTGCTGCATC CGACCTTCGA CAGCCCCAAC ACCCCTGGCG
     2221 TCCCCGCCCG CGCGCCGGAC ATCGTCGCCG GCCGCGACGG CCTCTACGCC CCGGACACCG
     2281 GCGGCACCTC GGTGTTCGAC CGCGCCGGCG TGCTGCGCCG CGCCGACGGC GACTTCGTGA
     2341 TACCCGACGG CACCGACATC CCGCCGGACC TTAAGGTGAA GCAGGACAGC TACAACAAGC
     2401 GCCTGCAAGC CACCCACTAC ACCATCATGC CGGCCAAGCC GATGTACCGG GAGGTCCTCA
     2461 TGGGCCAACT GGACAACTTC GTGCGCAACG CCATCCGCCG CCAATGGGAA AAAGCCCGCG
     2521 GGCTCTAGGA GTGAACCATG AACCTGAAAC CCCAGACCGA ACCATgagac cgctcagtgg
     2581 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2641 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2701 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2821 tctggcccca gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca
     2881 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3061 gcttcattca gctccggttc ccagcgatca aggcgagtta catgatcccc catgttgtgc
     3121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3361 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3541 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3601 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3661 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc ggtctcctgc
     3721 catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc
     3781 cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc
     3841 acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag
     3901 gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt
     3961 ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct
     4021 gcccctcaag
//

