LOCUS       pSAM_AraC               5906 bp    DNA     circular     11-APR-2017
FEATURES             Location/Qualifiers
     misc_feature    1252..1271
                     /note="Kan Forward"
     misc_feature    complement(2570..2589)
                     /note="Transposon verification primer"
     gene            complement(5907..6767)
                     /note="beta lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1459..2253
                     /note="Kan Resistance"
     misc_feature    1078..2592
                     /note="Transposon"
     misc_feature    1080..1099
                     /note="Tn Verification 3'"
     misc_feature    1078..1106
                     /note="MmeI IR_L"
     misc_feature    2563..2591
                     /note="MmeI IR_R"
     misc_feature    1467..1486
                     /note="KanFSB"
     misc_feature    complement(1928..1947)
                     /note="KanRSB"
     promoter        2650..2684
                     /note="Plac promoter"
     -35_signal      2651..2656
                     /note="-35 plac"
     -10_signal      2675..2680
                     /note="-10 plac"
     misc_feature    complement(2704..3582)
                     /note="araC"
     protein_bind    3612..3627
                     /note="O2"
     protein_bind    3769..3790
                     /note="O1"
     protein_bind    3810..3824
                     /note="CAP"
     protein_bind    3815..3858
                     /note="I2 and I1 region"
     -35_signal      3856..3860
                     /note="-35"
     -10_signal      3880..3884
                     /note="-10"
     gene            3927..4971
                     /note="Mariner himar1C9 transposase"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDW
                     YAKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERV
                     GHIIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDET
                     WLHHYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKT
                     INSDYYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPH
                     PPYSPDLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEG
                     RYNRCIALEGNYVE"
     misc_feature    3927..3929
                     /note="Start"
     rep_origin      5123..5507
                     /note="R6K gamma Ori"
     repeat_region   5690..5799
                     /note="OriT"
     terminator      2308..2524
                     /note=""
     promoter        complement(899..931)
                     /note="Pbla"
     primer_bind     1107..1127
                     /note="P7 illumina adapter "
     primer_bind     2542..2562
                     /note="P7 adapter sequence"
     promoter        3856..3889
                     /note="Pbad"
     source          1..5906
                     /dnas_title="pSAM_AraC"
ORIGIN      
        1 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
       61 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
      121 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
      181 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
      241 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
      301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
      361 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
      421 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
      481 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
      541 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
      601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
      661 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
      721 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
      781 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
      841 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
      901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
      961 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC
     1021 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CACGCGTCCT CGGTACCTAA
     1081 CAGGTTGGAT GATAAGTCCC CGGTCTTCGT ATGCCGTCTT CTGCTTGGCG CGCCCTCGAG
     1141 CAATTGagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     1201 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     1261 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     1321 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     1381 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     1441 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     1501 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     1561 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     1621 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     1681 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     1741 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     1801 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     1861 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     1921 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     1981 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     2041 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     2101 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     2161 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     2221 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgacTC
     2281 TAGAATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA
     2341 CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC
     2401 GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC
     2461 GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC
     2521 GTTTCTACCT GCAGGGCGCG CCAAGCAGAA GACGGCATAC GAAGACCGGG GACTTATCAT
     2581 CCAACCTGTT AGgatccagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2641 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2701 acaTTATGAC AACTTGACGG CTACATCATT CACTTTTTCT TCACAACCGG CACGGAACTC
     2761 GCTCGGGCTG GCCCCGGTGC ATTTTTTAAA TACCCGCGAG AAATAGAGTT GATCGTCAAA
     2821 ACCAACATTG CGACCGACGG TGGCGATAGG CATCCGGGTG GTGCTCAAAA GCAGCTTCGC
     2881 CTGGCTGATA CGTTGGTCCT CGCGCCAGCT TAAGACGCTA ATCCCTAACT GCTGGCGGAA
     2941 AAGATGTGAC AGACGCGACG GCGACAAGCA AACATGCTGT GCGACGCTGG CGATATCAAA
     3001 ATTGCTGTCT GCCAGGTGAT CGCTGATGTA CTGACAAGCC TCGCGTACCC GATTATCCAT
     3061 CGGTGGATGG AGCGACTCGT TAATCGCTTC CATGCGCCGC AGTAACAATT GCTCAAGCAG
     3121 ATTTATCGCC AGCAGCTCCG AATAGCGCCC TTCCCCTTGC CCGGCGTTAA TGATTTGCCC
     3181 AAACAGGTCG CTGAAATGCG GCTGGTGCGC TTCATCCGGG CGAAAGAACC CCGTATTGGC
     3241 AAATATTGAC GGCCAGTTAA GCCATTCATG CCAGTAGGCG CGCGGACGAA AGTAAACCCA
     3301 CTGGTGATAC CATTCGCGAG CCTCCGGATG ACGACCGTAG TGATGAATCT CTCCTGGCGG
     3361 GAACAGCAAA ATATCACCCG GTCGGCAAAC AAATTCTCGT CCCTGATTTT TCACCACCCC
     3421 CTGACCGCGA ATGGTGAGAT TGAGAATATA ACCTTTCATT CCCAGCGGTC GGTCGATAAA
     3481 AAAATCGAGA TAACCGTTGG CCTCAATCGG CGTTAAACCC GCCACCAGAT GGGCATTAAA
     3541 CGAGTATCCC GGCAGCAGGG GATCATTTTG CGCTTCAGCC ATACTTTTCA TACTCCCGCC
     3601 ATTCAGAGAA GAAACCAATT GTCCATATTG CATCAGACAT TGCCGTCACT GCGTCTTTTA
     3661 CTGGCTCTTC TCGCTAACCA AACCGGTAAC CCCGCTTATT AAAAGCATTC TGTAACAAAG
     3721 CGGGACCAAA GCCATGACAA AAACGCGTAA CAAAAGTGTC TATAATCACG GCAGAAAAGT
     3781 CCACATTGAT TATTTGCACG GCGTCACACT TTGCTATGCC ATAGCATTTT TATCCATAAG
     3841 ATTAGCGGAT CCTACCTGAC GCTTTTTATC GCAACTCTCT ACTGTTTCTC CATACCCGTT
     3901 TTTTGGGCTA ACAGGAGGAA TTAAcCATGG AAAAAAAGGA ATTTCGTGTT TTGATAAAAT
     3961 ACTGTTTTCT GAAGGGAAAA AATACAGTGG AAGCAAAAAC TTGGCTTGAT AATGAGTTTC
     4021 CGGACTCTGC CCCAGGGAAA TCAACAATAA TTGATTGGTA TGCAAAATTC AAGCGTGGTG
     4081 AAATGAGCAC GGAGGACGGT GAACGCAGTG GACGCCCGAA AGAGGTGGTT ACCGACGAAA
     4141 ACATCAAAAA AATCCACAAA ATGATTTTGA ATGACCGTAA AATGAAGTTG ATCGAGATAG
     4201 CAGAGGCCTT AAAGATATCA AAGGAACGTG TTGGTCATAT CATTCATCAA TATTTGGATA
     4261 TGCGGAAGCT CTGTGCGAAA TGGGTGCCGC GCGAGCTCAC ATTTGACCAA AAACAACGAC
     4321 GTGTTGATGA TTCTAAGCGG TGTTTGCAGC TGTTAACTCG TAATACACCC GAGTTTTTCC
     4381 GTCGATATGT GACAATGGAT GAAACATGGC TCCATCACTA CACTCCTGAG TCCAATCGAC
     4441 AGTCGGCTGA GTGGACAGCG ACCGGTGAAC CGTCTCCGAA GCGTGGAAAG ACTCAAAAGT
     4501 CCGCTGGCAA AGTAATGGCC TCTGTTTTTT GGGATGCGCA TGGAATAATT TTTATCGATT
     4561 ATCTTGAGAA GGGAAAAACC ATCAACAGTG ACTATTATAT GGCGTTATTG GAGCGTTTGA
     4621 AGGTCGAAAT CGCGGCAAAA CGGCCCCACA TGAAGAAGAA AAAAGTGTTG TTCCACCAAG
     4681 ACAACGCACC GTGCCACAAG TCATTGAGAA CGATGGCAAA AATTCATGAA TTGGGCTTCG
     4741 AATTGCTTCC CCACCCGCCG TATTCTCCAG ATCTGGCCCC CAGCGACTTT TTCTTGTTCT
     4801 CAGACCTCAA AAGGATGCTC GCAGGGAAAA AATTTGGCTG CAATGAAGAG GTGATCGCCG
     4861 AAACTGAGGC CTATTTTGAG GCAAAACCGA AGGAGTACTA CCAAAATGGT ATCAAAAAAT
     4921 TGGAAGGTCG TTATAATCGT TGTATCGCTC TTGAAGGGAA CTATGTTGAA TGCGGCCGCC
     4981 ACCGCGGTGG AGGGGAATTC AATTCGAAGC TTGGGCCCGA ACAAAAACTC ATCTCAGAAG
     5041 AGGATCTGAA TAGCGCCGTC GACCATCATC ATCATCATCA TTGAGTTTAA ACGGTCTCCA
     5101 GCTTGGCTGT TCGGTGGAGG GGAATTCCCA TGTCAGCCGT TAAGTGTTCC TGTGTCACTC
     5161 AAAATTGCTT TGAGAGGCTC TAAGGGCTTC TCAGTGCGTT ACATCCCTGG CTTGTTGTCC
     5221 ACAACCGTTA AACCTTAAAA GCTTTAAAAG CCTTATATAT TCTTTTTTTT CTTATAAAAC
     5281 TTAAAACCTT AGAGGCTATT TAAGTTGCTG ATTTATATTA ATTTTATTGT TCAAACATGA
     5341 GAGCTTAGTA CGTGAAACAT GAGAGCTTAG TACGTTAGCC ATGAGAGCTT AGTACGTTAG
     5401 CCATGAGGGT TTAGTTCGTT AAACATGAGA GCTTAGTACG TTAAACATGA GAGCTTAGTA
     5461 CGTGAAACAT GAGAGCTTAG TACGTACTAT CAACAGGTTG AACTGCTGAT CTTCAGATCC
     5521 TCTACGCCGG ACGCATCGTG GCCGGATCAA TTCCGTTTTC CGCTGCATAA CCCTGCTTCG
     5581 GGGTCATTAT AGCGATTTTT TCGGTATATC CATCCTTTTT CGCACGATAT ACAGGATTTT
     5641 GCCAAAGGGT TCGTGTAGAC TTTCCTTGGT GTATCCAACG GCGTCAGCCG GGCAGGATAG
     5701 GTGAAGTAGG CCCACCCGCG AGCGGGTGTT CCTTCTTCAC TGTCCCTTAT TCGCACCTGG
     5761 CGGTGCTCAA CGGGAATCCT GCTCTGCGAG GCTGGCCGGC TACCGCCGGC GTAACAGATG
     5821 AGGGCAAGCG GATGGCTGAT GAAACCAAGC CAACCAGGAA GGGCAGCCCA CCTATCACGG
     5881 AATTGATCCC CCTCGAATTG ACGCGT
//

