LOCUS       pSam_Cam4               4531 bp    DNA     circular     11-APR-2017
FEATURES             Location/Qualifiers
     primer_bind     1224..1243
                     /note="Kan Forward"
     primer_bind     complement(2542..2561)
                     /note="Transposon verification primer"
     gene            1431..2225
                     /note="Kan Resistance"
     misc_feature    1050..2564
                     /note="Transposon"
     primer_bind     1052..1071
                     /note="Tn Verification 3'"
     misc_feature    1050..1078
                     /note="MmeI IR_L"
     misc_feature    2535..2563
                     /note="MmeI IR_R"
     primer_bind     1439..1458
                     /note="KanFSB"
     misc_feature    complement(1900..1919)
                     /note="KanRSB"
     misc_feature    complement(27..60)
                     /note="distal 34-nt of of natural FRT site"
     CDS             complement(110..748)
                     /dnas_title="chloramphenicol acetyl transferase"
                     /note="Tn9"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyl transferase"
                     /protein_id="AAL02033.1"
                     /db_xref="GI:15554330"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR"
     misc_feature    complement(957..998)
                     /note="distal 42-nt of natural FRT site"
     primer_bind     2514..2534
                     /note="P7 illumina adapter"
     primer_bind     1079..1099
                     /note="P7 adapter sequence"
     repeat_region   4315..4424
                     /note="oriT"
     rep_origin      3748..4132
                     /note="R6K gamma ori"
     gene            2677..3721
                     /note="Mariner himar1C9 transposase"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDW
                     YAKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERV
                     GHIIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDET
                     WLHHYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKT
                     INSDYYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPH
                     PPYSPDLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEG
                     RYNRCIALEGNYVE"
     promoter        2622..2656
                     /note="Plac"
     promoter        complement(811..843)
                     /note="Pcat"
     terminator      2280..2496
                     /note=""
     source          1..4531
                     /dnas_title="pSam_Cam4"
ORIGIN      
        1 ACGCGTgtgt aggctggagc tgcttcgaag ttcctatact ttctagagaa taggaacttc
       61 ggaataggaa cttcatttaa atggcgcgcc ttacgccccg ccctgccact catcgcagta
      121 ctgttgtatt cattaagcat ctgccgacat ggaagccatc acaaacggca tgatgaacct
      181 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
      241 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
      301 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
      361 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
      421 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
      481 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgt aattccggat
      541 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
      601 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
      661 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
      721 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgaca
      781 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
      841 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
      901 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtagg cgcgccgaag
      961 ttcctatact ttctagagaa taggaacttc ggaataggaa ctaaggagga tattcatatg
     1021 gaccatggct aattcccatC CTCGGTACCT AACAGGTTGG ATGATAAGTC CCCGGTCTTC
     1081 GTATGCCGTC TTCTGCTTGG CGCGCCCTCG AGCAATTGag gaagcggaac acgtagaaag
     1141 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     1201 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     1261 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     1321 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     1381 ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     1441 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     1501 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     1561 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     1621 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     1681 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     1741 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     1801 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     1861 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     1921 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     1981 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     2041 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     2101 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2161 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     2221 tctgagcggg actctggggt tcgaaatgac TCTAGAATGC GAGAGTAGGG AACTGCCAGG
     2281 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG
     2341 TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG
     2401 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG
     2461 CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC CTGCAGGGCG CGCCAAGCAG
     2521 AAGACGGCAT ACGAAGACCG GGGACTTATC ATCCAACCTG TTAGgatcca gtgagcgcaa
     2581 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2641 ggctcgtatg ttgtgtggaa ttgtgagcgg atacaTATGG AAAAAAAGGA ATTTCGTGTT
     2701 TTGATAAAAT ACTGTTTTCT GAAGGGAAAA AATACAGTGG AAGCAAAAAC TTGGCTTGAT
     2761 AATGAGTTTC CGGACTCTGC CCCAGGGAAA TCAACAATAA TTGATTGGTA TGCAAAATTC
     2821 AAGCGTGGTG AAATGAGCAC GGAGGACGGT GAACGCAGTG GACGCCCGAA AGAGGTGGTT
     2881 ACCGACGAAA ACATCAAAAA AATCCACAAA ATGATTTTGA ATGACCGTAA AATGAAGTTG
     2941 ATCGAGATAG CAGAGGCCTT AAAGATATCA AAGGAACGTG TTGGTCATAT CATTCATCAA
     3001 TATTTGGATA TGCGGAAGCT CTGTGCGAAA TGGGTGCCGC GCGAGCTCAC ATTTGACCAA
     3061 AAACAACGAC GTGTTGATGA TTCTAAGCGG TGTTTGCAGC TGTTAACTCG TAATACACCC
     3121 GAGTTTTTCC GTCGATATGT GACAATGGAT GAAACATGGC TCCATCACTA CACTCCTGAG
     3181 TCCAATCGAC AGTCGGCTGA GTGGACAGCG ACCGGTGAAC CGTCTCCGAA GCGTGGAAAG
     3241 ACTCAAAAGT CCGCTGGCAA AGTAATGGCC TCTGTTTTTT GGGATGCGCA TGGAATAATT
     3301 TTTATCGATT ATCTTGAGAA GGGAAAAACC ATCAACAGTG ACTATTATAT GGCGTTATTG
     3361 GAGCGTTTGA AGGTCGAAAT CGCGGCAAAA CGGCCCCACA TGAAGAAGAA AAAAGTGTTG
     3421 TTCCACCAAG ACAACGCACC GTGCCACAAG TCATTGAGAA CGATGGCAAA AATTCATGAA
     3481 TTGGGCTTCG AATTGCTTCC CCACCCGCCG TATTCTCCAG ATCTGGCCCC CAGCGACTTT
     3541 TTCTTGTTCT CAGACCTCAA AAGGATGCTC GCAGGGAAAA AATTTGGCTG CAATGAAGAG
     3601 GTGATCGCCG AAACTGAGGC CTATTTTGAG GCAAAACCGA AGGAGTACTA CCAAAATGGT
     3661 ATCAAAAAAT TGGAAGGTCG TTATAATCGT TGTATCGCTC TTGAAGGGAA CTATGTTGAA
     3721 TAAGCGGCCG CCACCGCGGT GGAGGGGAAT TCCCATGTCA GCCGTTAAGT GTTCCTGTGT
     3781 CACTCAAAAT TGCTTTGAGA GGCTCTAAGG GCTTCTCAGT GCGTTACATC CCTGGCTTGT
     3841 TGTCCACAAC CGTTAAACCT TAAAAGCTTT AAAAGCCTTA TATATTCTTT TTTTTCTTAT
     3901 AAAACTTAAA ACCTTAGAGG CTATTTAAGT TGCTGATTTA TATTAATTTT ATTGTTCAAA
     3961 CATGAGAGCT TAGTACGTGA AACATGAGAG CTTAGTACGT TAGCCATGAG AGCTTAGTAC
     4021 GTTAGCCATG AGGGTTTAGT TCGTTAAACA TGAGAGCTTA GTACGTTAAA CATGAGAGCT
     4081 TAGTACGTGA AACATGAGAG CTTAGTACGT ACTATCAACA GGTTGAACTG CTGATCTTCA
     4141 GATCCTCTAC GCCGGACGCA TCGTGGCCGG ATCAATTCCG TTTTCCGCTG CATAACCCTG
     4201 CTTCGGGGTC ATTATAGCGA TTTTTTCGGT ATATCCATCC TTTTTCGCAC GATATACAGG
     4261 ATTTTGCCAA AGGGTTCGTG TAGACTTTCC TTGGTGTATC CAACGGCGTC AGCCGGGCAG
     4321 GATAGGTGAA GTAGGCCCAC CCGCGAGCGG GTGTTCCTTC TTCACTGTCC CTTATTCGCA
     4381 CCTGGCGGTG CTCAACGGGA ATCCTGCTCT GCGAGGCTGG CCGGCTACCG CCGGCGTAAC
     4441 AGATGAGGGC AAGCGGATGG CTGATGAAAC CAAGCCAACC AGGAAGGGCA GCCCACCTAT
     4501 CACGGAATTG ATCCCCCTCG AATTGACGCG T
//

