LOCUS       Exported                8765 bp ds-DNA     circular SYN 12-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pST39-pNL29_Addgene
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Mikhail Shapiro
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 12, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        187..205
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             453..719
                     /codon_start=1
                     /note="GvpB"
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     CDS             795..1061
                     /codon_start=1
                     /note="GvpR"
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     CDS             1090..2016
                     /codon_start=1
                     /note="GvpN"
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     CDS             2031..2798
                     /codon_start=1
                     /note="GvpF"
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     CDS             2800..3066
                     /codon_start=1
                     /note="GvpG"
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     CDS             3067..3876
                     /codon_start=1
                     /note="GvpL"
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     CDS             3894..4181
                     /codon_start=1
                     /note="GvpS"
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     CDS             4135..4419
                     /codon_start=1
                     /note="GvpK"
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     CDS             4441..4743
                     /codon_start=1
                     /note="GvpJ"
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     CDS             4780..5658
                     /codon_start=1
                     /note="GvpT"
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     CDS             5682..6098
                     /codon_start=1
                     /note="GvpU"
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     terminator      6368..6415
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6482..6573
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6574..7434
                     /codon_start=1
                     /note="Beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7508..8096
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8283..8422
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(8524..8715)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
       61 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
      121 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ggcaccatgc atcgatcccg
      181 cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga gatagcttag
      241 cgatgtgaaa gaagctggag aaaactttca agaaaaagtt tcttcttcag acgataaaga
      301 gaagtcaaaa aataagcgga aaataaaagg cgtaaatcag attaaaaaag caacggatat
      361 taaaagctct actaagatta aaagttctaa tgatattaaa tcatctacag atttaaaaac
      421 aatgggatca taagcgaaag gagaaatgag atatgtctat tcaaaaaagt actaatagtt
      481 caagtttagc agaagtcatt gaccgtattt tagataaagg aattgttatt gatgcttttg
      541 caagagtttc tgttgtagga attgaaattt taacgattga agcgcgagtg gttattgcca
      601 gtgttgatac atggttacgc tatgcagaag cagtagggct tcttcgtgac gacgtagaag
      661 aaaacggtct tcctgaacgt tcaaattcaa gtgaagggca gccgcgtttt agtatttaat
      721 atgaagtggt atggaacctt ccttttttga gggtggttcc agcttttaga tctaactatt
      781 ggaggctact aaaaatggaa attaaaaaaa ttatgcaagc cgtgaacgac tttttcggtg
      841 aacacgtagc tcctcctcat aaaattacct cggtggaagc tactgaagat gaaggttgga
      901 gagttattgt tgaagtcatt gaagaacgag aatatatgaa aaaatacgcc aaagatgaaa
      961 tgctcggaac gtacgagtgc tttgtaaata aagaaaaaga agtcatttca ttcaaacgac
     1021 tcgacgtcag atatagaagc gccattggca ttgaagcata aacgataaga tggcaggagg
     1081 aacgtaaaaa tgaccgtctt aacagacaaa aggaaaaaag gcagtggagc ttttatacaa
     1141 gatgacgaga caaaagaggt tctttcaaga gcgctgagct atttaaaatc cggctattcc
     1201 attcatttta caggtcctgc cggcggaggc aaaacctctt tagcgcgagc gcttgctaaa
     1261 aagagaaagc gtcctgtaat gctgatgcac gggaatcacg agctcaacaa caaagattta
     1321 attggcgatt ttacgggata cacgagcaaa aaagtaatcg accagtacgt tcgttctgtc
     1381 tataaaaaag atgaacaggt gagtgaaaac tggcaggatg gccgattgct tgaagctgta
     1441 aaaaatggct atacgctgat ttacgacgaa tttactcgtt ctaagcctgc gacgaataat
     1501 atctttctat cgatattaga agaaggcgtg ctgccgctgt atggagtaaa aatgaccgat
     1561 ccttttgtgc gcgtgcatcc cgatttccgc gtcatcttca caagcaatcc agctgagtat
     1621 gccggcgtat atgatacgca agatgcgctt ctcgacaggt taattaccat gtttattgat
     1681 tataaagaca tcgacagaga gacagcgatt ttaacggaga aaacggacgt agaagaagat
     1741 gaagcgcgca caattgtaac gctcgtagca aacgtgcgaa accgctctgg agacgaaaac
     1801 agcagcggac ttagcctgcg ggcttcgctt atgatcgcta cccttgccac gcagcaagac
     1861 attcctatcg atggaagtga cgaagatttt caaacgttat gtatcgatat tttgcatcat
     1921 ccgcttacca aatgtttgga tgaagaaaat gcaaaaagca aagccgaaaa aatcatttta
     1981 gaagaatgta agaatataga cactgaagaa aagtaaagga gcttgaaaac atgagtgaaa
     2041 caaacgaaac aggtatttat atttttagcg ccattcaaac ggataaagac gaagaatttg
     2101 gcgccgtgga agtagaagga acaaaagctg aaacattttt gattcgctac aaagacgcgg
     2161 ctatggtagc agctgaagta ccgatgaaaa tttatcatcc taatcgccaa aatttattaa
     2221 tgcatcaaaa cgcagtagca gcgattatgg acaagaacga tacggttatt ccaatcagct
     2281 ttgggaatgt attcaaatca aaagaagacg taaaagttct tttggaaaac ctttatccgc
     2341 agtttgaaaa gctgtttcca gcgatcaaag gaaaaattga agtcggttta aaagtaattg
     2401 ggaaaaaaga atggcttgag aaaaaagtaa acgaaaatcc tgaacttgag aaagtatcag
     2461 catccgtaaa aggaaaatca gaagcagccg gttattatga gcgtattcaa cttggaggaa
     2521 tggctcaaaa gatgtttact tccctgcaaa aagaagtcaa gacagatgtg ttttctccgc
     2581 ttgaagaagc agcggaagca gcaaaagcaa atgagccaac gggcgaaacg atgcttttaa
     2641 acgcgtcttt cttaattaac cgagaagatg aagcgaagtt tgatgaaaaa gtaaatgaag
     2701 cgcatgaaaa ctggaaagac aaagccgatt ttcattacag cggtccttgg cctgcttata
     2761 attttgtgaa cattcgccta aaagtagaag agaaataacg tgcttcacaa attagtaacc
     2821 gcacccatta accttgtagt gaaaatcggc gaaaaagtac aggaagaagc tgataaacag
     2881 ctatatgacc ttccgacgat tcagcaaaag ctcattcagc ttcaaatgat gtttgagctt
     2941 ggtgaaattc cagaagaagc gtttcaagaa aaagaagatg aattgttaat gaggtacgaa
     3001 attgcgaaac gcagagaaat tgaacaatgg gaagagctaa cacaaaaaag aaatgaggaa
     3061 tcctagatgg gagaattact gtatttatac ggtttaattc caacaaaaga agcagcagcc
     3121 atagagccgt ttccatctta taaggggttt gacggagaac attcactgta cccaattgcg
     3181 tttgatcagg tgacggctgt agtttctaag ctggatgctg acacctattc agaaaaagtg
     3241 attcaagaaa aaatggagca ggatatgagc tggctgcagg aaaaagcatt tcatcatcac
     3301 gaaacggtag ccgctttgta cgaagaattt acgatcattc cattaaaatt ttgcaccatt
     3361 tataaaggtg aagaaagtct gcaggcagct attgagatta acaaagaaaa gatagagaat
     3421 tcactgacgc tgcttcaagg aaatgaagag tggaatgtga aaatttactg tgatgataca
     3481 gagcttaaaa aaggaatcag cgaaacgaat gaaagcgtga aagcgaaaaa acaagaaatt
     3541 agtcacttat caccaggaag acagtttttt gaaaagaaaa aaatagatca gctgattgaa
     3601 aaagaattag agcttcacaa aaacaaagtg tgtgaagaga tacatgacaa gctaaaagaa
     3661 ttatcgcttt atgactctgt taaaaagaat tggagcaaag acgtaactgg cgcagctgaa
     3721 cagatggcgt ggaacagcgt gtttcttctc ccgtctctgc agattactaa gttcgtaaac
     3781 gaaatagaag agcttcagca aaggcttgaa aataaaggct ggaagtttga agtgacggga
     3841 ccatggccgc cctatcattt ctcgagcttt gcgtaaagtg aggaattaac attatgtctc
     3901 ttaaacaatc catggagaat aaagatattg ctcttattga tattttagat gtcattttag
     3961 ataaaggagt cgccattaaa ggagacttaa tcatttccat agctggcgtc gatttagtgt
     4021 atttggattt gcgggtgctt atttcttcgg ttgaaacgct tgtgcaagca aaagaaggaa
     4081 atcacaaacc aatcacttct gaacaatttg ataaacaaaa ggaggaatta atggatgcaa
     4141 ccggtcagcc aagcaaatgg acgaatccac ttggatcctg atcaagctga acaaggctta
     4201 gcgcagcttg tgatgacagt tattgagcta ttgaggcaaa tcgttgaacg tcatgccatg
     4261 aggcgggtgg agggtggaac gttgacggac gaacaaattg aaaacttagg aattgcacta
     4321 atgaacttag aagaaaaaat ggacgagttg aaagaggtgt tcggtctgga tgcagaagat
     4381 ttaaatattg atcttggacc gctaggcagc ctgctttaag cggtcagtag gaggaacagt
     4441 atggcagtcg aacataatat gcagtcaagt acgattgtag atgtgctcga aaagattttg
     4501 gataaaggag tcgttatagc gggggacatc accgtaggaa ttgcagatgt cgagctatta
     4561 acgataaaga tccgcttgat tgtggcttcg gttgataagg caaaagaaat cggcatggac
     4621 tggtgggaaa atgatccgta tctcagttca aaaggagcca ataacaaagc gctcgaagaa
     4681 gaaaataaaa tgctgcatga gcggttaaaa acgcttgaag aaaaaataga aacgaaacgt
     4741 taaaaactgt acgctactta aaaaatggag ggatttacaa tggcaactga aacaaaatta
     4801 gataacacac aggcagaaaa caaggaaaat aaaaatgcgg aaaacggttc aaaagaaaag
     4861 aacggttcaa aagcaagcaa aacaacaagc agcgggccaa tcaaacgagc ggtagcagga
     4921 ggcatcatcg gtgcaacgat tggatatgta tcgactcctg aaaatcgaaa aagtctcctt
     4981 gaccgcattg atacagacga attaaaaagc aaagcatctg atttaggaac aaaggtaaaa
     5041 gaaaaatcaa aaagcagcgt ggccagcctg aaaacatctg cgggaagctt gtttaaaaaa
     5101 gataaagata aatcaaaaga tgatgaagaa aacgtaaatt cttctagtag cgaaacagaa
     5161 gacgataacg ttcaagagta cgacgagtta aaagaagaaa atcaaactct tcaagatcgc
     5221 ttatcacagc ttgaagaaaa aatgaacatg cttgttgagc ttagcctcaa taaaaatcaa
     5281 gacgaagaag cggaagatac agattccgac gaagaagaga acgatgagaa cgatgaaaac
     5341 gatgaaaacg agcaggacga tgaaaacgaa gaagaaacat ctaagccacg taaaaaggat
     5401 aaaaaagaag ctgaggaaga agaaagtgaa agtgacgaag acagcgagga agaagaggaa
     5461 gattctcgct caaacaaaaa aaataaaaaa gtaaaaacag aagaagaaga cgaagatgaa
     5521 agcgaagaag aaaaaaagga agcgaaacca aaaaagtcaa cagctaaaaa atcaaaaaat
     5581 acaaaagcaa agaaaaacac ggacgaagaa gatgatgaag caacatctct ttctagtgaa
     5641 gacgatacaa cagcctaaga cgtaaaggag gaaagaaaga catgagtaca ggcccttctt
     5701 tttcaactaa agacaatacg cttgaatact ttgtgaaagc ttctaataaa cacggctttt
     5761 cacttgatat ttcattaaat gtaaacggcg ctgtgatttc cggtaccatg atttcagcaa
     5821 aagaatactt tgattactta agcgaaacgt ttgaagaagg cagtgaagtg gctcaggcgc
     5881 taagcgaaca attctcttta gcaagcgaag cgagcgaatc aaacggagaa gcagaagccc
     5941 attttattca tttgaaaaat acaaagattt actgtggaga cagtaaatct actccttcta
     6001 aaggcaaaat cttttggaga gggaaaatag cagaagtaga cgggtttttc ttaggaaaga
     6061 tttctgatgc aaaatcaacg agtaaaaaga gttcataaaa aacggcgggg tgattgcccc
     6121 gccgtttttt agtgatgtga tgagatgtgc agcttctttt ttcgatacat gagcggagtc
     6181 atcttcattt cttttcgaaa ctgattaata aaatagcttg tactattaaa ccctacttga
     6241 taggcgactt ccgtcacatt tgcttctgct tgctgcagtc cggattcgcg aacgcgtctc
     6301 gagagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     6361 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     6421 aggaggaact atatccgact agtagcccgc ctaatgagcg ggcttttttt taattcccct
     6481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctcgtg
     6661 aaagtaaaag acgcagagga ccaattgggg gcacgagtgg gatacataga actggacttg
     6721 aatagcggta aaatccttga gagttttcgc cctgaagagc gttttccaat gatgagcact
     6781 ttcaaagttc tgctatgtgg agcagtatta tcccgtgtag atgcggggca agagcaactc
     6841 ggacgacgaa tacactattc gcagaatgac ttggttgaat actccccagt gacagaaaag
     6901 caccttacgg acggaatgac ggtaagagaa ttatgtagtg ccgccataac gatgagtgat
     6961 aacactgcgg cgaacttact tctgacaacc atcggtggac cgaaggaatt aaccgctttt
     7021 ttgcacaata tgggagacca tgtaactcgc cttgaccgtt gggaaccaga actgaatgaa
     7081 gccataccaa acgacgagcg agacaccaca atgcctgcgg caatggcaac aacattacgc
     7141 aaactattaa ctggcgaact acttactctg gcttcacggc aacaattaat agactggctt
     7201 gaagcggata aagttgcagg accactactg cgttcggcac ttcctgctgg ctggtttatt
     7261 gctgataaat ctggggcagg agagcgtggt tcacggggta tcattgccgc acttggacca
     7321 gatggtaagc cttcccgtat cgtagttatc tacacgacgg gtagtcaggc aactatggac
     7381 gaacgaaata gacagattgc tgaaataggg gcttcactga ttaagcattg gtaaaccgat
     7441 acaattaaag gctccttttg gagccttttt ttttggacgg accggtagaa aagatcaaag
     7501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7621 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8101 ccagcaacgc ggcccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8161 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8221 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8281 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
     8341 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     8401 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     8461 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     8521 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     8581 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     8641 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     8701 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg ggtaatgata
     8761 ccgat
//
