LOCUS Exported 5775 bp ds-DNA circular SYN 02-JUN-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS 91719CaMKIIP-mEGFP-P2A-paAIP2(R5AR6A) _pAAV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5775) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 2, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5775 /organism="synthetic DNA construct" /note="dnas_title=HM18-34 CaMKIIP-mG-P2A-LOV2AIP2(R5AR6A) _pAAV" misc_feature 1..130 /label=L-ITR /note="dnas_title=L-ITR vntifkey=21" promoter 170..1463 /label=CaMKII 1.3 promoter /note="dnas_title=CaMKII 1.3 promoter vntifkey=29" exon 1492..2208 /label=mGFP(A206K) /note="dnas_title=mGFP(A206K) vntifkey=61" misc_feature 2218..2274 /label=P2A /note="dnas_title=P2A vntifkey=21" misc_feature 2284..2691 /label=LOV2(404-539) /note="dnas_title=LOV2(404-539) vntifkey=21" misc_feature 2692..2730 /label=AIP2 /note="dnas_title=AIP2 vntifkey=21" polyA_signal 2740..2998 /label=hGH polyA /note="dnas_title=hGH polyA vntifkey=25" misc_feature 3038..3178 /label=R-ITR /note="dnas_title=R-ITR vntifkey=21" promoter 3270..3570 /label=f1 origin /note="dnas_title=f1 origin vntifkey=29" promoter 4095..4952 /label=Amp /note="dnas_title=Amp vntifkey=29" promoter 5103..5770 /label=pUC origin /note="dnas_title=pUC origin vntifkey=29" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtattacc gccatgcatt agttattaat taacattatg 181 gccttaggtc acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg 241 ggtgcagaga gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa 301 cttgaactca gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac 361 agaaggagtg aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc 421 agggtacatc tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat 481 agttgcctct ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc 541 ccaggagacc aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag 601 gcctcaggag gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt 661 cattccgtca gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga 721 tatgcacaat ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc 781 aagtcaacca gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg 841 aagggaagga gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg 901 caacggggca atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt 961 ctttctcaag gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc 1021 tttgccccac ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag 1081 gtaggaagag cgatgatcac ttgtggacta agtttgttcg catccccttc tccaaccccc 1141 tcagtacatc accctggggg aacagggtcc acttgctcct gggcccacac agtcctgcag 1201 tattgtgtat ataaggccag ggcaaaggag gagcaggttt taaagtgaaa ggcaggcagg 1261 tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg 1321 ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa 1381 gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag 1441 tggcccctag ttctgggggc agcgggggat ccccgggtac cggtcgccac catggtgagc 1501 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 1561 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 1621 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 1681 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 1741 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 1801 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 1861 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 1921 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 1981 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 2041 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 2101 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 2161 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aagcggagcc 2221 actaacttct ccctgttgaa acaagcaggg gatgtcgaag agaatcccgg gccaggatcc 2281 atgctggcga ccaccctgga acgcattgaa aaaaactttg tgattaccga tccgcgcctg 2341 ccggataacc cgattatttt tgcgagcgat agctttctgc agctgaccga atatagccgc 2401 gaagaaattc tgggccgcaa ctgccgcttt ctgcagggcc cggaaaccga tcgcgcgacc 2461 gtgcgcaaaa ttcgcgatgc gattgataac cagaccgaag tgaccgtgca gctgattaac 2521 tataccaaaa gcggcaaaaa attttggaac ctgtttcatc tgcagccgat gcgcgatcag 2581 aaaggcgatg tgcagtattt tattggcgtg cagctggatg gcaccgaaca tgtgcgcgat 2641 gcggcggaac gcgaaggcgt gatgctgatt aaaaaaaccg cggaaaacat taagaagaaa 2701 ctggctgcac aggaagcgtt tgatgcgctg taaaagcttg tggcatccct gtgacccctc 2761 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat 2821 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag 2881 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta 2941 ttgggaaccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct 3001 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg 3061 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 3121 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg 3181 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa 3241 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 3301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 3361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 3421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt 3481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 3541 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt 3601 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3661 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc 3721 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 3781 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 3841 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 3901 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 3961 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 4021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 4081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 4141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 4201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 4261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 4321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 4381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 4441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 4501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 4561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 4621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 4681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 4741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 4801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 4861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 4921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 4981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 5041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 5101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 5161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 5221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 5281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 5401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 5461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 5521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 5581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 5641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 5701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 5761 cttttgctca catgt //