LOCUS       Exported                5775 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    91719CaMKIIP-mEGFP-P2A-paAIP2(R5AR6A) _pAAV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="synthetic DNA construct"
                     /note="dnas_title=HM18-34 CaMKIIP-mG-P2A-LOV2AIP2(R5AR6A) 
                     _pAAV"
     misc_feature    1..130
                     /label=L-ITR
                     /note="dnas_title=L-ITR
                     vntifkey=21"
     promoter        170..1463
                     /label=CaMKII 1.3 promoter
                     /note="dnas_title=CaMKII 1.3 promoter
                     vntifkey=29"
     exon            1492..2208
                     /label=mGFP(A206K)
                     /note="dnas_title=mGFP(A206K)
                     vntifkey=61"
     misc_feature    2218..2274
                     /label=P2A
                     /note="dnas_title=P2A
                     vntifkey=21"
     misc_feature    2284..2691
                     /label=LOV2(404-539)
                     /note="dnas_title=LOV2(404-539)
                     vntifkey=21"
     misc_feature    2692..2730
                     /label=AIP2
                     /note="dnas_title=AIP2
                     vntifkey=21"
     polyA_signal    2740..2998
                     /label=hGH polyA
                     /note="dnas_title=hGH polyA
                     vntifkey=25"
     misc_feature    3038..3178
                     /label=R-ITR
                     /note="dnas_title=R-ITR
                     vntifkey=21"
     promoter        3270..3570
                     /label=f1 origin
                     /note="dnas_title=f1 origin
                     vntifkey=29"
     promoter        4095..4952
                     /label=Amp
                     /note="dnas_title=Amp
                     vntifkey=29"
     promoter        5103..5770
                     /label=pUC origin
                     /note="dnas_title=pUC origin
                     vntifkey=29"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattacc gccatgcatt agttattaat taacattatg
      181 gccttaggtc acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg
      241 ggtgcagaga gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa
      301 cttgaactca gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac
      361 agaaggagtg aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc
      421 agggtacatc tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat
      481 agttgcctct ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc
      541 ccaggagacc aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag
      601 gcctcaggag gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt
      661 cattccgtca gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga
      721 tatgcacaat ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc
      781 aagtcaacca gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg
      841 aagggaagga gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg
      901 caacggggca atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt
      961 ctttctcaag gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc
     1021 tttgccccac ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag
     1081 gtaggaagag cgatgatcac ttgtggacta agtttgttcg catccccttc tccaaccccc
     1141 tcagtacatc accctggggg aacagggtcc acttgctcct gggcccacac agtcctgcag
     1201 tattgtgtat ataaggccag ggcaaaggag gagcaggttt taaagtgaaa ggcaggcagg
     1261 tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg
     1321 ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa
     1381 gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag
     1441 tggcccctag ttctgggggc agcgggggat ccccgggtac cggtcgccac catggtgagc
     1501 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1561 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1621 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1681 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1741 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1801 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1861 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1921 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1981 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2041 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2101 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2161 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aagcggagcc
     2221 actaacttct ccctgttgaa acaagcaggg gatgtcgaag agaatcccgg gccaggatcc
     2281 atgctggcga ccaccctgga acgcattgaa aaaaactttg tgattaccga tccgcgcctg
     2341 ccggataacc cgattatttt tgcgagcgat agctttctgc agctgaccga atatagccgc
     2401 gaagaaattc tgggccgcaa ctgccgcttt ctgcagggcc cggaaaccga tcgcgcgacc
     2461 gtgcgcaaaa ttcgcgatgc gattgataac cagaccgaag tgaccgtgca gctgattaac
     2521 tataccaaaa gcggcaaaaa attttggaac ctgtttcatc tgcagccgat gcgcgatcag
     2581 aaaggcgatg tgcagtattt tattggcgtg cagctggatg gcaccgaaca tgtgcgcgat
     2641 gcggcggaac gcgaaggcgt gatgctgatt aaaaaaaccg cggaaaacat taagaagaaa
     2701 ctggctgcac aggaagcgtt tgatgcgctg taaaagcttg tggcatccct gtgacccctc
     2761 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     2821 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     2881 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     2941 ttgggaaccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     3001 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     3061 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     3121 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     3181 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     3241 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     3481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3541 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     3601 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3661 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     3721 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     3781 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     3841 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3901 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3961 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5761 cttttgctca catgt
//
