LOCUS       Exported                9621 bp ds-DNA     circular SYN 14-APR-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLEX_305-C-dTAG
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Justin Roberts
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 15, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        16..516
                     /note="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    537..661
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: RIGHT"
     promoter        686..716
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
                     /note="This forward directional feature has 3 segments:
                        1: 686 .. 691 / #ffffff / -35
                        2: 692 .. 709 / #ffffff
                        3: 710 .. 716 / #ffffff / -10"
     CDS             770..1429
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1771..2076
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2117..2241)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             2249..2569
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /note="FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    2354..2356
                     /note="F36V mutation"
                     /note="color: #ff0000"
     CDS             2576..2602
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             2606..2632
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     misc_feature    2654..3242
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(3125..3136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 3124"
                     /translation="IEGR"
     LTR             3314..3547
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    3625..3746
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      3786..3921
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(3942..3960)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(3970..3986)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      4128..4583
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4609..4713
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4714..5574
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4714 .. 4782 / #ccffcc / signal sequence
                        2: 4783 .. 5574 / #ccffcc
                     Cleavage site after base 4782"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5745..6333
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    6621..6642
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        6657..6687
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 6657 .. 6662 / #ffffff / -35
                        2: 6663 .. 6680 / #ffffff
                        3: 6681 .. 6687 / #ffffff / -10"
     protein_bind    6695..6711
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     6719..6735
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        6756..6774
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6802..7028
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             7029..7209
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    7256..7381
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    7874..8107
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     promoter        8589..8785
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8636..8771
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             8817..9419
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     misc_feature    9462..9579
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
       61 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      121 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      181 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      241 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      301 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      361 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      421 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      481 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatcgat tgatcaacaa
      541 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      601 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      661 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      721 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      781 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      841 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      901 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      961 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     1021 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1081 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1141 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1201 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1261 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1321 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1381 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc
     1441 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     1501 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     1561 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     1621 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     1681 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     1741 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     1801 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     1861 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     1921 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     1981 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     2041 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     2101 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     2161 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     2221 agctttcttg tacaaagtgg ttaccggtgg agtgcaggtg gaaaccatct ccccaggaga
     2281 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga
     2341 agatggaaag aaagttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg
     2401 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag
     2461 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat
     2521 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaag gcggctaccc
     2581 ctacgacgtg cccgactacg ccggctatcc gtatgatgtc ccggactatg caggctaaac
     2641 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2701 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2761 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2821 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2881 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2941 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3001 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3061 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3121 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3181 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3241 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3301 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3361 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3421 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3481 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3541 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     3601 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     3661 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3721 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     3781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3841 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3901 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     3961 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     4141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4561 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     4621 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4681 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4741 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4801 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4861 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4921 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4981 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5041 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5101 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5161 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5221 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5281 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5341 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5401 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5461 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5521 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5581 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5641 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5881 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6361 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6421 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6481 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6541 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6601 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6661 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6721 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6781 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     6841 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     6901 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     6961 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     7021 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7081 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7141 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7201 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7261 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7321 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7381 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7441 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7501 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7561 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7621 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7681 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7741 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7801 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7861 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7921 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7981 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     8041 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     8101 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     8161 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     8221 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     8281 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     8341 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     8401 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     8461 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca
     8521 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8581 gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8641 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8701 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8761 ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga
     8821 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8881 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8941 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     9001 gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg
     9061 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     9121 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     9181 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     9241 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     9301 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     9361 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac
     9421 gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     9481 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     9541 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     9601 agagatccac tttggcgccg g
//
