LOCUS mKate2_C1_donor 3410 bp ds-DNA circular 23-AUG-2016 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(1158..1175) /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 163..179 /label=attB1 /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 20..40 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(1755..2441) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 1048..1057 /label=attB2r /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1129..1149) /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(2511..3317) /label=KanR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 188..190 /label=ATG Overlap /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 180..186 /label=SapI /ApEinfo_fwdcolor=#ea17ff /ApEinfo_revcolor=#ea17ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 58..77 /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1077..1123,86..150) /label=MCS-inverted in SK+ /ApEinfo_fwdcolor=#bc27cf /ApEinfo_revcolor=#bc27cf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1041..1047 /label=SapI(1) /ApEinfo_label=SapI /ApEinfo_fwdcolor=#ea17ff /ApEinfo_revcolor=#ea17ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 130..135 /label=smaI /ApEinfo_fwdcolor=#ff5375 /ApEinfo_revcolor=#ff5375 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(191..391,443..601,653..817,869..1036) /label=mKate2 /ApEinfo_fwdcolor=#993333 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1077..1079,148..150) /label=EcoRV /ApEinfo_fwdcolor=#ff5375 /ApEinfo_revcolor=#ff5375 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 94..103 /label=MslI /ApEinfo_fwdcolor=#ff5375 /ApEinfo_revcolor=#ff5375 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1037..1039 /label=AAG overlap /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 AGACACGGGC CAGAGCTGCc aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 61 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact 121 agtggatccc ccgggctgca ggaattcgat GGGGACAAGT TTGTACAAAA AAGCAGGCTG 181 CTCTTCgATG GTCTCCGAGC TCATTAAAGA AAACATGCAT ATGAAGCTCT ACATGGAGGG 241 AACCGTCAAC AACCACCACT TCAAGTGCAC CTCCGAGGGA GAGGGAAAGC CATACGAGGG 301 AACCCAAACC ATGCGTATCA AGGCCGTCGA GGGAGGACCA CTCCCATTCG CCTTCGACAT 361 CCTCGCCACC TCCTTCATGT ACGGATCCAA Ggtaagttta aacatatata tactaactaa 421 ccctgattat ttaaattttc agACCTTCAT CAACCACACC CAAGGAATCC CAGACTTCTT 481 CAAGCAATCC TTCCCAGAGG GATTCACCTG GGAGCGTGTC ACCACCTACG AGGACGGAGG 541 AGTCCTCACC GCCACCCAAG ACACCTCCCT CCAAGACGGA TGCCTCATCT ACAACGTCAA 601 Ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agATCCGTGG 661 AGTCAACTTC CCATCCAACG GACCAGTCAT GCAAAAGAAG ACCCTCGGAT GGGAGGCCTC 721 CACCGAGACC CTCTACCCAG CCGACGGAGG ACTTGAGGGA CGTGCCGACA TGGCCCTCAA 781 GCTCGTCGGA GGAGGACACC TCATCTGCAA CCTCAAGgta agtttaaaca tgattttact 841 aactaactaa tctgatttaa attttcagAC CACCTACCGT TCCAAGAAGC CAGCCAAGAA 901 CCTCAAGATG CCAGGAGTCT ACTACGTCGA CCGTCGTCTT GAGCGTATCA AGGAGGCCGA 961 CAAGGAGACC TACGTCGAGC AACACGAGGT CGCCGTCGCC CGTTACTGCG ACCTCCCATC 1021 CAAGCTCGGA CACCGTAAGc GAAGAGCACC CAGCTTTCTT GTACAAAGTG GTCCCCatca 1081 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag 1141 tcgtattacg cgcgctcact ggccgtcgtt ttacaACGTC GTGACTGGGA AAACATCCAT 1201 GCTAGCGTTA ACGCGAGAGT AGGGAACTGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC 1261 GGAAGACTGG GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC 1321 AAATCCGCCG GGAGCGGATT TGAACGTTGT GAAGCAACGG CCCGGAGGGT GGCGGGCAGG 1381 ACGCCCGCCA TAAACTGCCA GGCATCAAAC TAAGCAGAAG GCCATCCTGA CGGATGGCCT 1441 TTTTGCGTTT CTACAAACTC TTCCTGGCTA GCGGTACGCG TATTAATTGC GTTGCGCTCA 1501 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 1561 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 1621 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 1681 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 1741 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 1801 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 1861 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 1921 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 1981 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 2041 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 2101 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 2161 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 2221 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 2281 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 2341 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 2401 TGGAACGACG CGTAACTCAC GTTAAGGGAT TTTGGTCATG AGCTTGCGCC GTCCCGTCAA 2461 GTCAGCGTAA TGCTCTGCCA GTGTTACAAC CAATTAACCA ATTCTGATTA GAAAAACTCA 2521 TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA 2581 AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA 2641 TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC 2701 TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG 2761 AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG 2821 TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA 2881 CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC 2941 AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC 3001 TGGAATGCTG TTTTTCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG 3061 ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG TCTGACCATC 3121 TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA 3181 TCGGGCTTCC CATACAAGCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC 3241 CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT CGACGTTTCC 3301 CGTTGAATAT GGCTCATAAC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT 3361 GTTCATGATG ATATATTTTT ATCTTGTGCA ATGTAACATC AGAGATTTTG //