LOCUS       mKate2_C1_donor         3410 bp ds-DNA     circular     23-AUG-2016
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(1158..1175)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     163..179
                     /label=attB1
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     20..40
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(1755..2441)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     1048..1057
                     /label=attB2r
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1129..1149)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2511..3317)
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    188..190
                     /label=ATG Overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    180..186
                     /label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     58..77
                     /label=T3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1077..1123,86..150)
                     /label=MCS-inverted in SK+
                     /ApEinfo_fwdcolor=#bc27cf
                     /ApEinfo_revcolor=#bc27cf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1041..1047
                     /label=SapI(1)
                     /ApEinfo_label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    130..135
                     /label=smaI
                     /ApEinfo_fwdcolor=#ff5375
                     /ApEinfo_revcolor=#ff5375
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(191..391,443..601,653..817,869..1036)
                     /label=mKate2
                     /ApEinfo_fwdcolor=#993333
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1077..1079,148..150)
                     /label=EcoRV
                     /ApEinfo_fwdcolor=#ff5375
                     /ApEinfo_revcolor=#ff5375
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    94..103
                     /label=MslI
                     /ApEinfo_fwdcolor=#ff5375
                     /ApEinfo_revcolor=#ff5375
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1037..1039
                     /label=AAG overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 AGACACGGGC CAGAGCTGCc aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
       61 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact
      121 agtggatccc ccgggctgca ggaattcgat GGGGACAAGT TTGTACAAAA AAGCAGGCTG
      181 CTCTTCgATG GTCTCCGAGC TCATTAAAGA AAACATGCAT ATGAAGCTCT ACATGGAGGG
      241 AACCGTCAAC AACCACCACT TCAAGTGCAC CTCCGAGGGA GAGGGAAAGC CATACGAGGG
      301 AACCCAAACC ATGCGTATCA AGGCCGTCGA GGGAGGACCA CTCCCATTCG CCTTCGACAT
      361 CCTCGCCACC TCCTTCATGT ACGGATCCAA Ggtaagttta aacatatata tactaactaa
      421 ccctgattat ttaaattttc agACCTTCAT CAACCACACC CAAGGAATCC CAGACTTCTT
      481 CAAGCAATCC TTCCCAGAGG GATTCACCTG GGAGCGTGTC ACCACCTACG AGGACGGAGG
      541 AGTCCTCACC GCCACCCAAG ACACCTCCCT CCAAGACGGA TGCCTCATCT ACAACGTCAA
      601 Ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agATCCGTGG
      661 AGTCAACTTC CCATCCAACG GACCAGTCAT GCAAAAGAAG ACCCTCGGAT GGGAGGCCTC
      721 CACCGAGACC CTCTACCCAG CCGACGGAGG ACTTGAGGGA CGTGCCGACA TGGCCCTCAA
      781 GCTCGTCGGA GGAGGACACC TCATCTGCAA CCTCAAGgta agtttaaaca tgattttact
      841 aactaactaa tctgatttaa attttcagAC CACCTACCGT TCCAAGAAGC CAGCCAAGAA
      901 CCTCAAGATG CCAGGAGTCT ACTACGTCGA CCGTCGTCTT GAGCGTATCA AGGAGGCCGA
      961 CAAGGAGACC TACGTCGAGC AACACGAGGT CGCCGTCGCC CGTTACTGCG ACCTCCCATC
     1021 CAAGCTCGGA CACCGTAAGc GAAGAGCACC CAGCTTTCTT GTACAAAGTG GTCCCCatca
     1081 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     1141 tcgtattacg cgcgctcact ggccgtcgtt ttacaACGTC GTGACTGGGA AAACATCCAT
     1201 GCTAGCGTTA ACGCGAGAGT AGGGAACTGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC
     1261 GGAAGACTGG GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC
     1321 AAATCCGCCG GGAGCGGATT TGAACGTTGT GAAGCAACGG CCCGGAGGGT GGCGGGCAGG
     1381 ACGCCCGCCA TAAACTGCCA GGCATCAAAC TAAGCAGAAG GCCATCCTGA CGGATGGCCT
     1441 TTTTGCGTTT CTACAAACTC TTCCTGGCTA GCGGTACGCG TATTAATTGC GTTGCGCTCA
     1501 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC
     1561 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
     1621 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
     1681 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
     1741 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG
     1801 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
     1861 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
     1921 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
     1981 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
     2041 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
     2101 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
     2161 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA
     2221 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
     2281 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
     2341 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2401 TGGAACGACG CGTAACTCAC GTTAAGGGAT TTTGGTCATG AGCTTGCGCC GTCCCGTCAA
     2461 GTCAGCGTAA TGCTCTGCCA GTGTTACAAC CAATTAACCA ATTCTGATTA GAAAAACTCA
     2521 TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA
     2581 AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA
     2641 TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC
     2701 TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG
     2761 AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG
     2821 TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA
     2881 CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC
     2941 AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC
     3001 TGGAATGCTG TTTTTCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG
     3061 ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG TCTGACCATC
     3121 TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA
     3181 TCGGGCTTCC CATACAAGCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC
     3241 CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT CGACGTTTCC
     3301 CGTTGAATAT GGCTCATAAC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT
     3361 GTTCATGATG ATATATTTTT ATCTTGTGCA ATGTAACATC AGAGATTTTG 
//
