LOCUS 381_SapTrap_Pmyo 4793 bp ds-DNA circular 12-JUL-2016 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from SapTrap FP 13 to 916 COMMENT Backbone from 084_pDONR221 2897 to 651 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 652..668 /label=attB1 /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator complement(268..295) /label=rrnB_T2_terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 2918..2927 /label=attB2r /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator complement(427..470) /label=rrnB_T1_terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 669..675 /label=SapI /ApEinfo_fwdcolor=#ea17ff /ApEinfo_revcolor=#ea17ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2911..2917 /label=SapI(1) /ApEinfo_label=SapI /ApEinfo_fwdcolor=#ea17ff /ApEinfo_revcolor=#ea17ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 570..651 /label=attP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2907..2909 /label=AAG overlap /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2928..3016) /label=attP(1) /ApEinfo_label=attP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter complement(3035..3053) /label=T7_promoter /ApEinfo_fwdcolor=#33cc00 /ApEinfo_revcolor=#33cc00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3054..3072) /label=M13_reverse_primer /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 4127..4746 /label=pBR322_origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(3054..3074) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(3034..3055) /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3187..3993 /label=KanR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 536..553 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(1642..1812,1864..2017,2069..2232,2284..2508) /label=GFP(65C) /ApEinfo_fwdcolor=green /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 680..1641 /label=Pmyo-2 /ApEinfo_fwdcolor=#33cc00 /ApEinfo_revcolor=#33cc00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'_UTR 2542..2906 /label=myo-2 3pUTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 677..679 /label=ATG overlap /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTGC TCTTCgATGA TTTTATATCT GAGTAGTATC CTTTGCTTTA AATGTCCATA 721 AAACTAATTT TATAATCAAT AAAACAACGT TTGTAAATCA ACTGAGTTTA CAAGTAGAGA 781 CATTGAGGGA TACTTTCACT ATGCTAAAGT GATAATCGAC CAAATAATAA CTTCACTTTG 841 GTATTTATTC CTGTCTTATA AATGTTATGT ATGAATTAAA TTCATATGCA TATGGCTCAC 901 TCTGACAATT TTTAATAATC TTCCAGATCA ATATTGACTA CCGATGCGGG TGGTCTTTTG 961 CTTTGAATTC TGCTGAACTT TACACCCCGA ACAGCAATGT GTGCTTCAGC CTAAAAAAAA 1021 GTAAGTGTGT TAATCAGTGC CCCCGATTCT TCATTTTTTG CCCCTCTCTC CCGTTTCGTC 1081 GGCAAAAGAA GAGAAAATAA AGATAAGTCT CAAGATAGGT TGGTAATCGC TAAAGTGGTT 1141 GTGTGGATAA GAGTAGCAAA ATGGCAGcAA GAGCACTTTG CGCGCACACA CTGTACTCAT 1201 TGTTCTGGAT AAAATTCTCT CGTTGTTTGC CGTCGGATGT CTGCCTCTCT GCATTGAGCC 1261 GGCTTCTTCA CTATCTTTAG TTAACCTAAA ATGCCGTTTC TTTTCTCGTA TCCCCACTAT 1321 CCCGTTGAGG TTCTCTGCTC TCTTCGCTCC CTACCGCCAG CGAGCAACTA TCCGTGGGGG 1381 CGCCTTGCTC GGAAGATGGG GGGGAAGAAA GAAGATTTTT GCTATTTGCA CTTGAGAAAG 1441 AGACTTTTCC TGCGTCGATG GTTAGAGAAC AGTGTGCAGA CACTTTTCAG CTACCTAGAA 1501 TTACAATTGG ATATCCCCGC CTCCCAATCC ACCCACCCAG GGAAAAAGAA GGGCTCGCCG 1561 AAAATCAAAG TTATCTCCAG GCTCGCGCAT CCCACCGAGC GGTTGACTTC TCTCCACCAC 1621 TTTTCATTTT TACCCTCGAC CATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA 1681 ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT 1741 GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA 1801 CCTGTTCCAT GGGTAAGTTT AAACATATAT ATACTAACTA ACCCTGATTA TTTAAATTTT 1861 CAGCCAACAC TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTCTCGAG ATACCCAGAT 1921 CATATGAAAC GGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT ACAGGAAAGA 1981 ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACGTA AGTTTAAACA GTTCGGTACT 2041 AACTAACCAT ACATATTTAA ATTTTCAGGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT 2101 GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC 2161 AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT 2221 GGAATCAAAG TTGTAAGTTT AAACATGATT TTACTAACTA ACTAATCTGA TTTAAATTTT 2281 CAGAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT 2341 CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC 2401 ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG 2461 TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC TATACAAATA GATCAACTGC 2521 TCTAATTCCA TCTCGTTTCT CTCTTTTCTT TCAATCTCTT TACCCGTTTC TCTTCCCCTA 2581 CGTCCCCCAA ACCTACCACT GCACAGTAAC ACATACACAT TCAACGTGTC ACAATAAAAG 2641 CCCATCAACA CCTTCTCTGT TGTTTTTTTT CTTCAAATGC ATTTTTTTCA CTTTGTTTAA 2701 TAATTTTTAC GCATCGGATT TGTAGGATAG TGGAAATTTG TCCTTCAATG CTTCAATTGA 2761 TTCGACACTT CTCAATATTT TTGATCAATA AACCATTGAT TTTCTATTTA GAAGAAGATC 2821 ACTGTAAATA ACACCAAAAA CATCACAGCA AAAAAACTCC CACTCAATTA TATTACTGTT 2881 TTTATTGAAA CTAGAATAAA TTTGAAAAGc GAAGAGCACC CAGCTTTCTT GTACAAAGTT 2941 GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA 3001 AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC 3061 ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA 3121 GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG 3181 GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT 3241 GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC 3301 TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC 3361 GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT 3421 CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG 3481 ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT 3541 GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT 3601 TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG 3661 GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA 3721 GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA 3781 CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC 3841 GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT 3901 CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA 3961 TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA 4021 CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT 4081 AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 4141 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 4201 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 4261 TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 4321 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 4381 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 4441 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 4501 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA 4561 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 4621 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 4681 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 4741 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //