LOCUS       381_SapTrap_Pmyo        4793 bp ds-DNA     circular     12-JUL-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from SapTrap FP 13 to 916
COMMENT     Backbone from 084_pDONR221 2897 to 651
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     652..668
                     /label=attB1
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(268..295)
                     /label=rrnB_T2_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2918..2927
                     /label=attB2r
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(427..470)
                     /label=rrnB_T1_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    669..675
                     /label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2911..2917
                     /label=SapI(1)
                     /ApEinfo_label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    570..651
                     /label=attP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2907..2909
                     /label=AAG overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2928..3016)
                     /label=attP(1)
                     /ApEinfo_label=attP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(3035..3053)
                     /label=T7_promoter
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3054..3072)
                     /label=M13_reverse_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4127..4746
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3054..3074)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3034..3055)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3187..3993
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     536..553
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1642..1812,1864..2017,2069..2232,2284..2508)
                     /label=GFP(65C)
                     /ApEinfo_fwdcolor=green
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        680..1641
                     /label=Pmyo-2
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'_UTR          2542..2906
                     /label=myo-2 3pUTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    677..679
                     /label=ATG overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTGC TCTTCgATGA TTTTATATCT GAGTAGTATC CTTTGCTTTA AATGTCCATA
      721 AAACTAATTT TATAATCAAT AAAACAACGT TTGTAAATCA ACTGAGTTTA CAAGTAGAGA
      781 CATTGAGGGA TACTTTCACT ATGCTAAAGT GATAATCGAC CAAATAATAA CTTCACTTTG
      841 GTATTTATTC CTGTCTTATA AATGTTATGT ATGAATTAAA TTCATATGCA TATGGCTCAC
      901 TCTGACAATT TTTAATAATC TTCCAGATCA ATATTGACTA CCGATGCGGG TGGTCTTTTG
      961 CTTTGAATTC TGCTGAACTT TACACCCCGA ACAGCAATGT GTGCTTCAGC CTAAAAAAAA
     1021 GTAAGTGTGT TAATCAGTGC CCCCGATTCT TCATTTTTTG CCCCTCTCTC CCGTTTCGTC
     1081 GGCAAAAGAA GAGAAAATAA AGATAAGTCT CAAGATAGGT TGGTAATCGC TAAAGTGGTT
     1141 GTGTGGATAA GAGTAGCAAA ATGGCAGcAA GAGCACTTTG CGCGCACACA CTGTACTCAT
     1201 TGTTCTGGAT AAAATTCTCT CGTTGTTTGC CGTCGGATGT CTGCCTCTCT GCATTGAGCC
     1261 GGCTTCTTCA CTATCTTTAG TTAACCTAAA ATGCCGTTTC TTTTCTCGTA TCCCCACTAT
     1321 CCCGTTGAGG TTCTCTGCTC TCTTCGCTCC CTACCGCCAG CGAGCAACTA TCCGTGGGGG
     1381 CGCCTTGCTC GGAAGATGGG GGGGAAGAAA GAAGATTTTT GCTATTTGCA CTTGAGAAAG
     1441 AGACTTTTCC TGCGTCGATG GTTAGAGAAC AGTGTGCAGA CACTTTTCAG CTACCTAGAA
     1501 TTACAATTGG ATATCCCCGC CTCCCAATCC ACCCACCCAG GGAAAAAGAA GGGCTCGCCG
     1561 AAAATCAAAG TTATCTCCAG GCTCGCGCAT CCCACCGAGC GGTTGACTTC TCTCCACCAC
     1621 TTTTCATTTT TACCCTCGAC CATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA
     1681 ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT
     1741 GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA
     1801 CCTGTTCCAT GGGTAAGTTT AAACATATAT ATACTAACTA ACCCTGATTA TTTAAATTTT
     1861 CAGCCAACAC TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTCTCGAG ATACCCAGAT
     1921 CATATGAAAC GGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT ACAGGAAAGA
     1981 ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACGTA AGTTTAAACA GTTCGGTACT
     2041 AACTAACCAT ACATATTTAA ATTTTCAGGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT
     2101 GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC
     2161 AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT
     2221 GGAATCAAAG TTGTAAGTTT AAACATGATT TTACTAACTA ACTAATCTGA TTTAAATTTT
     2281 CAGAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT
     2341 CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC
     2401 ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG
     2461 TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC TATACAAATA GATCAACTGC
     2521 TCTAATTCCA TCTCGTTTCT CTCTTTTCTT TCAATCTCTT TACCCGTTTC TCTTCCCCTA
     2581 CGTCCCCCAA ACCTACCACT GCACAGTAAC ACATACACAT TCAACGTGTC ACAATAAAAG
     2641 CCCATCAACA CCTTCTCTGT TGTTTTTTTT CTTCAAATGC ATTTTTTTCA CTTTGTTTAA
     2701 TAATTTTTAC GCATCGGATT TGTAGGATAG TGGAAATTTG TCCTTCAATG CTTCAATTGA
     2761 TTCGACACTT CTCAATATTT TTGATCAATA AACCATTGAT TTTCTATTTA GAAGAAGATC
     2821 ACTGTAAATA ACACCAAAAA CATCACAGCA AAAAAACTCC CACTCAATTA TATTACTGTT
     2881 TTTATTGAAA CTAGAATAAA TTTGAAAAGc GAAGAGCACC CAGCTTTCTT GTACAAAGTT
     2941 GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA
     3001 AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC
     3061 ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA
     3121 GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG
     3181 GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT
     3241 GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC
     3301 TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC
     3361 GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT
     3421 CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG
     3481 ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT
     3541 GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT
     3601 TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG
     3661 GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA
     3721 GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA
     3781 CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC
     3841 GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT
     3901 CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA
     3961 TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA
     4021 CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT
     4081 AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     4141 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     4201 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     4261 TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     4321 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     4381 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     4441 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     4501 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
     4561 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     4621 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     4681 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     4741 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT
//
