LOCUS       Untitled                7722 bp ds-DNA     circular SYN 22-MAY-2017
DEFINITION  pCFJ601 - Peft-3 Mos1 transposase.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 22, 2017 from SnapGene 3.3.4 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     VNTDATE|806457600|
COMMENT     VNTDBDATE|806457600|
COMMENT     VNTNAME|Untitled|
COMMENT     VNTAUTHORNAME|Addgene|
FEATURES             Location/Qualifiers
     CDS             join(1..1044,1096..2166,2218..3243,3295..4323)
                     /label=Cas9
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     3'UTR           4330..4661
                     /label=tbb-2 3'UTR
                     /note="ApEinfo_fwdcolor=#cc3333
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#cc3333"
     primer_bind     complement(4642..4661)
                     /label=tbb2T R #68
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_recomb     complement(4662..4682)
                     /label=attB3
                     /note="ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#dda0dd"
     misc_feature    complement(4669..4675)
                     /label=Gateway Recomb3
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0000"
     misc_feature    4687..4842
                     /label=lacZ_a
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     complement(4690..4707)
                     /label=M13-fwd
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     promoter        complement(4690..4706)
                     /label=M13_forward20_primer
                     /label=M13_forward20_primer promoter
                     /note="ApEinfo_fwdcolor=#33cc00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#33cc00"
     misc_feature    complement(4699..4721)
                     /label=M13_pUC_fwd_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     complement(4707..4726)
                     /label=Gateway Promoter L #73
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     primer_bind     4777..4796
                     /label=U6 Vector 3' #162
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#00cc99"
     CDS             4778..4846
                     /label=LacZ alpha
                     /note="ApEinfo_fwdcolor=#6495ed
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#6495ed"
     misc_feature    5034..5056
                     /label=pGEX_3_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     promoter        5215..5243
                     /label=AmpR_promoter
                     /note="ApEinfo_fwdcolor=#33cc00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#33cc00"
     CDS             5483..6145
                     /codon_start=1
                     /label=ORF frame 1
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     CDS             5483..6142
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=yellow
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=yellow"
     misc_feature    5489..6145
                     /gene="Ampicillin (variant)"
                     /label=Ampicillin (variant)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     rep_origin      6240..6922
                     /label=ColE1 origin
                     /note="ApEinfo_fwdcolor=gray50
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=gray50"
     rep_origin      6300..6919
                     /label=pBR322_origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    7031..7053
                     /label=M13_pUC_rev_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     7050..7070
                     /label=M13-rev
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     promoter        7052..7070
                     /label=M13_reverse_primer
                     /label=M13_reverse_primer promoter
                     /note="ApEinfo_fwdcolor=#33cc00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#33cc00"
     misc_recomb     7086..7106
                     /label=attB4
                     /note="ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#dda0dd"
     misc_feature    7093..7099
                     /label=Gateway Recomb4
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0000"
     promoter        7107..7714
                     /label=Peft3
                     /label=Peft3 promoter
                     /note="ApEinfo_fwdcolor=#33cc00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#33cc00"
ORIGIN
        1 atggacaaaa aatacagcat cggccttgac atcggaacga actccgtcgg atgggccgtc
       61 atcaccgacg agtacaaggt cccatccaag aagttcaagg tcctcggaaa caccgaccgt
      121 cactccatca agaagaacct catcggagcc ctcctcttcg actccggaga gaccgccgag
      181 gccacccgtc tcaagcgtac cgcccgtcgt cgttacaccc gtcgtaagaa ccgtatctgc
      241 tacctccaag agatcttctc caacgagatg gccaaggtcg acgactcctt cttccaccgt
      301 ctcgaggagt ccttcctcgt cgaggaggac aagaagcacg agcgtcaccc aatcttcgga
      361 aacatcgtcg acgaggtcgc ctaccacgag aagtacccaa ccatctacca cctccgtaag
      421 aagctcgtcg actccaccga caaggccgac ctccgtctca tctacctcgc cctcgcccac
      481 atgatcaagt tccgtggaca cttcctcatc gagggagacc tcaacccaga caactccgac
      541 gtcgacaagc tcttcatcca actcgtccaa acctacaacc aactcttcga ggagaaccca
      601 atcaacgcct ccggagtcga cgccaaggcc atcctctccg cccgtctctc caagtcccgt
      661 cgtctcgaga acctcatcgc ccaactccca ggagagaaga agaacggact cttcggaaac
      721 ctcatcgccc tctccctcgg actcacccca aacttcaagt ccaacttcga cctcgccgag
      781 gacgccaagc tccaactctc caaggacacc tacgacgacg acctcgacaa cctcctcgcc
      841 caaatcggag accaatacgc cgacctcttc ctcgccgcca agaacctctc cgacgccatc
      901 ctcctctccg acatcctccg tgtcaacacc gagatcacca aggccccact ctccgcctcc
      961 atgatcaagc gttacgacga gcaccaccaa gacctcaccc tcctcaaggc cctcgtccgt
     1021 caacaactcc cagagaagta caaggtaagt ttaaacatat atatactaac taaccctgat
     1081 tatttaaatt ttcaggagat cttcttcgac caatccaaga acggatacgc cggatacatc
     1141 gacggaggag cctcccaaga ggagttctac aagttcatca agccaatcct cgagaagatg
     1201 gacggaaccg aggagctcct cgtcaagctc aaccgtgagg acctcctccg taagcaacgt
     1261 accttcgaca acggatccat cccacaccaa atccacctcg gagagctcca cgccatcctc
     1321 cgtcgtcaag aggacttcta cccattcctc aaggacaacc gtgagaagat cgagaagatc
     1381 ctcaccttcc gtatcccata ctacgtcgga ccactcgccc gtggaaactc ccgtttcgcc
     1441 tggatgaccc gtaagtccga ggagaccatc accccatgga acttcgagga ggtcgtcgac
     1501 aagggagcct ccgcccaatc cttcatcgag cgtatgacca acttcgacaa gaacctccca
     1561 aacgagaagg tcctcccaaa gcactccctc ctctacgagt acttcaccgt ctacaacgag
     1621 ctcaccaagg tcaagtacgt caccgaggga atgcgtaagc cagccttcct ctccggagag
     1681 caaaagaagg ccatcgtcga cctcctcttc aagaccaacc gtaaggtcac cgtcaagcaa
     1741 ctcaaggagg actacttcaa gaagatcgag tgcttcgact ccgtcgagat ctccggagtc
     1801 gaggaccgtt tcaacgcctc cctcggaacc taccacgacc tcctcaagat catcaaggac
     1861 aaggacttcc tcgacaacga ggagaacgag gacatcctcg aggacatcgt cctcaccctc
     1921 accctcttcg aggaccgtga gatgatcgag gagcgtctca agacctacgc ccacctcttc
     1981 gacgacaagg tcatgaagca gcttaagcgt cgtcgttaca ccggatgggg acgtctctcc
     2041 cgtaagctca tcaacggaat ccgtgacaag caatccggaa agaccatcct cgacttcctc
     2101 aagtccgacg gattcgccaa ccgtaacttc atgcaactca tccacgacga ctccctcacc
     2161 ttcaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggag
     2221 gacatccaaa aggcccaagt ctccggacaa ggagactccc tccacgagca catcgccaac
     2281 ctcgccggat ccccagccat caagaaggga atcctccaaa ccgtcaaggt cgtcgacgag
     2341 ctcgtcaagg tcatgggacg tcacaagcca gagaacatcg tcatcgagat ggcccgtgag
     2401 aaccaaacca cccaaaaggg acaaaagaac tcccgtgagc gtatgaagcg tatcgaggag
     2461 ggaatcaagg agctcggatc ccaaatcctc aaggagcacc cagtcgagaa cacccaactc
     2521 caaaacgaga agctctacct ctactacctc caaaacggac gtgacatgta cgtcgaccaa
     2581 gagctcgaca tcaaccgtct ctccgactac gacgtcgacc acatcgtccc acaatccttc
     2641 ctcaaggacg actccatcga caacaaggtc ctcacccgtt ccgacaagaa ccgtggaaag
     2701 tccgacaacg tcccatccga ggaggtcgtc aagaagatga agaactactg gcgtcaactc
     2761 ctcaacgcca agctcatcac ccaacgtaag ttcgacaacc tcaccaaggc tgagcgtgga
     2821 ggactctccg agctcgacaa ggccggattc atcaagcgtc aactcgtcga gacccgtcaa
     2881 atcaccaagc acgtcgccca aatcctcgac tcccgtatga acaccaagta cgacgagaac
     2941 gacaagctca tccgtgaggt caaggtcatc accctcaagt ccaagctcgt ctccgacttc
     3001 cgtaaggact tccaattcta caaggtccgt gagatcaaca actaccacca cgcccacgac
     3061 gcctacctca acgccgtagt aggaaccgcc ctcatcaaga agtacccaaa gctcgagtcc
     3121 gagttcgtct acggagacta caaggtctac gacgtccgta agatgatcgc caagtccgag
     3181 caagagatcg gaaaggccac cgccaagtac ttcttctact ccaacatcat gaacttcttc
     3241 aaggtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaccgag
     3301 atcaccctcg ccaacggaga gatccgtaag cgtccactca tcgagaccaa cggagagacc
     3361 ggagagatcg tctgggacaa gggacgtgac ttcgccacag tccgtaaggt cctctccatg
     3421 ccacaagtca acatcgtcaa gaagaccgag gtccaaaccg gaggattctc caaggagtcc
     3481 atcctcccaa agcgtaactc cgacaagctc atcgcccgta agaaggactg ggacccaaag
     3541 aagtacggag gattcgactc cccaaccgtc gcctactccg tcctcgtcgt cgccaaggtc
     3601 gagaagggaa agtccaagaa gctcaagtcc gtcaaggagc tcctcggaat caccatcatg
     3661 gagcgttcct ccttcgagaa gaacccaatc gacttcctcg aggccaaggg atacaaggag
     3721 gtcaagaagg acctcatcat caagctccca aagtactccc tcttcgagct cgagaacgga
     3781 cgtaagcgta tgctcgcctc cgccggagag ctccaaaagg gaaacgagct cgccctccca
     3841 tccaagtacg tcaacttcct ctacctcgcc tcccactacg agaagctcaa gggatcccca
     3901 gaggacaacg agcaaaagca actcttcgtc gagcaacaca agcactacct cgacgagatc
     3961 atcgagcaaa tctccgagtt ctccaagcgt gtcatcctcg ccgacgccaa cctcgacaag
     4021 gtcctctccg cctacaacaa gcaccgtgac aagccaatcc gtgagcaagc cgagaacatc
     4081 atccacctct tcaccctcac caacctcgga gccccagccg ccttcaagta cttcgacacc
     4141 accatcgacc gtaagcgtta cacctccacc aaggaggtcc tcgacgccac cctcatccac
     4201 caatccatca ccggactcta cgagacccgt atcgacctct cccaactcgg aggagacgga
     4261 ggatccggac caccaaagaa gaagcgtaag gtctacccat acgacgtccc agactacgcc
     4321 taaactagtg ataaatgcaa aatcctttca agcattccct tcttctctat cactcttctt
     4381 tctttttgtc aaaaaattct ctcgctaatt tatttgcttt tttaatgtta ttattttatg
     4441 actttttata gtcactgaaa agtttgcatc tgagtgaagt gaatgctatc aaaatgtgat
     4501 tctgtctgat gtactttcac aatctctctt caattccatt ttgaagtgct ttaaacccga
     4561 aaggttgaga aaaatgcgag cgctcaaata tttgtattgt gttcgttgag tgacccaaca
     4621 aaaagaggaa actttattgt gccgccaaga aaaaagtctc acaactttat tatacatagt
     4681 tgataattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     4861 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4921 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4981 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5041 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5101 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5281 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5341 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5401 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5461 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5521 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5581 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5641 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5701 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5761 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5821 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5881 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5941 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6001 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6061 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6121 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6181 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6241 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6421 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6721 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggtc ttttgctggc cttttgctca
     6961 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7021 agctgatacc agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7081 gctatcaact ttgtatagaa aagttggcac ctttggtctt ttattgtcaa cttccattgg
     7141 ttcttccatt gtttctgtta aattaatgaa tttttcataa aataaagaca ttatacaata
     7201 taaaaatgaa gaatttattg aaaataaact gccagagaga aaaagtatgc aacactcccg
     7261 ccgagagtgt ttgaaatggt gtacggtaca ttttcgtgct aggagttaga tgtgcaggca
     7321 gcaacgagag ggggagagat ttttttgggc cttgtgaaat taacgtgagt tttctggtca
     7381 tctgactaat catgttggtt ttttgttggt ttattttgtt tttatctttg tttttatcca
     7441 gattaggaaa tttaaatttt atgaatttat aatgaggtca aacattcagt cccagcgttt
     7501 ttcctgttct cactgtttag tcgaattttt attttaggct ttcaacaaat gttctaactg
     7561 tcttatttgt gacctcactt tttatatttt tttaattttt aaaaatatta gaagtttcta
     7621 ggataatttt ttcgactttt attctctcta ccgtccgcac tcttcttact tttaaattaa
     7681 attgtttttt tttcagttgg gaaacacttt gctctagaaa aa
//
