LOCUS       IGI_P0613_pGL4_2        4739 bp ds-DNA     circular     13-APR-2017
DEFINITION  Firefly luciferase reporter vector pGL4.23[luc2/minP], complete
            sequence.
ACCESSION   DQ904455
VERSION     DQ904455.1  GI:115342874
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.23[luc2/minP]
  ORGANISM  Firefly luciferase reporter vector pGL4.23[luc2/minP] other
            sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     pGL4 Luciferase reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(1..19,476..526)
                     /note="multiple cloning region"
                     /label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      534..564
                     /regulatory_class="promoter"
                     /note="minimal promoter"
                     /label=minimal promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            597..2249
                     /gene="luc2"
                     /label=luc2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             597..2249
                     /gene="luc2"
                     /note="reporter gene"
                     /codon_start=1
                     /transl_table=11
                     /product="firefly luciferase"
                     /protein_id="ABI94439.1"
                     /db_xref="GI:115342875"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label=firefly luciferase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      2284..2505
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
                     /label=SV40 late poly(A) signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2573..2592
                     /note="reporter vector primer 4 binding region"
                     /label=reporter vector primer 4 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2830..2830
                     /note="ColE1-derived plasmid replication origin"
                     /label=ColE1-derived plasmid replication origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(3621..4481)
                     /gene="Amp(r)"
                     /label=Amp(r)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3621..4481)
                     /gene="Amp(r)"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="ABI94440.1"
                     /db_xref="GI:115342876"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=synthetic beta-lactamase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      4586..4739
                     /regulatory_class="polyA_signal_sequence"
                     /note="poly(A) signal/transcriptional pause site"
                     /label=poly(A) signal/transcriptional pause site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     4688..4707
                     /note="RVprimer3 binding region"
                     /label=RVprimer3 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    20..469
                     /label=IL2RA CaRE5 minus strand
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggcctaactg gccggtaccA ATATGACTCC ATCCCAAGAA ATTGGTTTTA AAGATTTGAG
       61 GAAAAACTGC TTTTAGATTT CAGAGAAAGA AAAAGAGTGT GTTTCTACTT CCCCCTTTTA
      121 AATCTGATAA CTTTTATTTT AATCAGGTTG ACATTCTGTG GTGAGCTTGT GGTTAACTCT
      181 TTCATGCCCG GAAGCCTGTT GTCGACCGAG AAGACTCCCA GGATTAGAAC ATAACCAGCG
      241 CCCAACATTT CATTTGCAGA AGCATCTGAG GTCCCAAAAT GTGATGGTTT AGCGGCAGCT
      301 GCTTGTAGGG GTATTTTTGG TTTCCTGCTT CTTTTTTCCC TTCTAAGTAA AGACAAAATA
      361 ACTAGATCCC TCCACCCCAC CACCCCATCA TTTCGTTGTT GTTTAACAAG TGTTGAGTAA
      421 GGCACAGTTG TTCTCCAAAA GGGTCTTACC CTGTGAGTTA CTCATTTTAg gtacctgagc
      481 tcgctagcct cgaggatatc aagatctggc ctcggcggcc aagcttagac actagagggt
      541 atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccatgg
      601 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
      661 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
      721 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
      781 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
      841 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
      901 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
      961 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     1021 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     1081 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     1141 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     1201 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     1261 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     1321 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     1381 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     1441 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     1501 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     1561 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     1621 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     1681 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     1741 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     1801 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
     1861 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     1921 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     1981 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     2041 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     2101 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     2161 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     2221 ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc
     2281 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2341 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2401 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2461 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2521 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2581 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2641 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3241 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa
     3601 atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc
     3661 tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt
     3721 gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc
     3781 cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct
     3841 actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg
     3901 ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg
     3961 tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca
     4021 cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag
     4081 ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg
     4141 ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag
     4201 tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat
     4261 agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg
     4321 atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca
     4381 gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca
     4441 aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat
     4501 tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag
     4561 gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt
     4621 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     4681 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctct
//
