LOCUS       IGI_P0616_pGL4_2        5319 bp ds-DNA     circular     13-APR-2017
DEFINITION  Firefly luciferase reporter vector pGL4.23[luc2/minP], complete
            sequence.
ACCESSION   DQ904455
VERSION     DQ904455.1  GI:115342874
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.23[luc2/minP]
  ORGANISM  Firefly luciferase reporter vector pGL4.23[luc2/minP] other
            sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     pGL4 Luciferase reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(1..19,1056..1106)
                     /note="multiple cloning region"
                     /label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1050..1055)
                     /note="multiple cloning region"
                     /label=multiple cloning region(1)
                     /ApEinfo_label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      1114..1144
                     /regulatory_class="promoter"
                     /note="minimal promoter"
                     /label=minimal promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1177..2829
                     /gene="luc2"
                     /label=luc2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1177..2829
                     /gene="luc2"
                     /note="reporter gene"
                     /codon_start=1
                     /transl_table=11
                     /product="firefly luciferase"
                     /protein_id="ABI94439.1"
                     /db_xref="GI:115342875"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label=firefly luciferase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      2864..3085
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
                     /label=SV40 late poly(A) signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     3153..3172
                     /note="reporter vector primer 4 binding region"
                     /label=reporter vector primer 4 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3410..3410
                     /note="ColE1-derived plasmid replication origin"
                     /label=ColE1-derived plasmid replication origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(4201..5061)
                     /gene="Amp(r)"
                     /label=Amp(r)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(4201..5061)
                     /gene="Amp(r)"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="ABI94440.1"
                     /db_xref="GI:115342876"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=synthetic beta-lactamase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      5166..5319
                     /regulatory_class="polyA_signal_sequence"
                     /note="poly(A) signal/transcriptional pause site"
                     /label=poly(A) signal/transcriptional pause site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5268..5287
                     /note="RVprimer3 binding region"
                     /label=RVprimer3 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(20..322,324..766,768..1049)
                     /label=IL2RA CaRE6 plus strand
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    323..323
                     /label=rs7893467
                     /ApEinfo_fwdcolor=#9e28ff
                     /ApEinfo_revcolor=#9e28ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    767..767
                     /label=rs11256448
                     /ApEinfo_fwdcolor=#9e28ff
                     /ApEinfo_revcolor=#9e28ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggcctaactg gccggtaccA AAGAGATGTG GGGGACAGTC ATTTTAGAGA GCGGACACCA
       61 CTGCTGAAGA AGAAGGATAT CCCGGTTGGC TGCTCTAGAG CCGGCAGGAC GGATGCTGAG
      121 GTTGTTCGCA GCAGCGTGTG TGCAGAGCCC CCGGGATCTT GCAGACTGGG ATTTGTCAGG
      181 GCAGGTGTGG ACATTGAGGC TATTTCTAAC AAGCTCCTAC ATGATGCTGG GGCTGCAGAG
      241 CCCCAGGCCG CACTTGGAGT ATGAGAGTTT CCAGGGAGTG AAGCCCCGGC TGCTCTCTCT
      301 GCCCCCTCTC CATCTGCCCC CTtGGTGAGG ACAGGACTCC CATCCCTCCA GTGCACAGTG
      361 AGTGCTGCTC AATCTGCATC CACAGCGGTT TGTGCCCAGA TGCTTCCTCA CTCAGCGTCT
      421 AACAGGGTGC AGTGGAAACG TCATTGACTG GAGGAAAGGT GTGTGGGTGA GAAACGTTTG
      481 TCGGTTGAGT AACTTTTTGT CGTTATTTTT TTTCTCTGAT GTTCAAAGTA AGAGAAAACT
      541 CATCTGGCCC CTGGAGCCCA TGTGAGCAAC ATGAAAGATA AAGGGAAAAG GGGGCTGATA
      601 AAGATGTGGA CCGAGTCTGA GATAAGATTC TTTGCCCCTT GGGTGAATCC AGGTGAAGTT
      661 GTTTTACTCT TAGCCCAGCC AGCCCTGGAT AAAGGCTTCA TCCCTCTTCT CTTTCCTGTC
      721 CATATATCAG AACTTTAAGC TCTACTTGTA CCCAGTGAAG ATTAGAgTAA CGCACGGAGC
      781 ACAGGAAATG TCTAGGTGAT GATTTCACTG AGCTTTACAA GGGACACAGG TACGCATCAA
      841 GAGCTTGGGC CACTGGGTAA AGAGGGATCT GGTTCTTGCT CTAACGCGGT TCATCTTCTG
      901 TGTCAGAATC AGATAGATTC TGGGTGTCAG AGACCCGAAT CTTCCTCTTG TTCCGCTCAC
      961 TGCCCCCCTG TGGCCCCTTC TCATTTCAAA TGCTCTGTCT GTAATATGAA ATGAAAAGAA
     1021 TTCCTCCCAG ACCCCTAGCC AAATTTCACg gtacctgagc tcgctagcct cgaggatatc
     1081 aagatctggc ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt
     1141 ccagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag
     1201 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
     1261 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
     1321 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
     1381 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
     1441 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
     1501 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
     1561 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
     1621 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
     1681 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
     1741 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
     1801 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
     1861 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
     1921 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     1981 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     2041 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     2101 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     2161 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     2221 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     2281 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
     2341 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
     2401 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
     2461 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
     2521 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
     2581 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
     2641 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
     2701 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
     2761 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
     2821 gccgtgtaat aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
     2881 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2941 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3001 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     3061 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag
     3121 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3181 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     3241 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3301 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3361 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3421 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3481 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3541 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3601 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3661 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3721 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3781 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3841 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3901 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3961 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4021 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4081 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4141 aaagtatata tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg
     4201 ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat
     4261 agtggcctga ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc
     4321 cagcgcagca atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa
     4381 ccagccagca gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca
     4441 gtctatgagc tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag
     4501 agttgtggcc attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt
     4561 caactctggt tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc
     4621 agtcagctcc ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct
     4681 catggtaatg gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc
     4741 cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg
     4801 ctcttgcccg gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct
     4861 catcatcggg aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc
     4921 cagttcgata tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag
     4981 cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac
     5041 acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg
     5101 ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag
     5161 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     5221 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     5281 ctgtccccag tgcaagtgca ggtgccagaa catttctct  
//
